MMP-12 Antibody

Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MMP-12 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary.
• Localization: Secreted , extracellular space , extracellular matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MMP-12 Antibody

• EC 3.4.24
• hME
• HMEEC 3.4.24.65
• Macrophage elastase
• macrophage metalloelastase
• matrix metallopeptidase 12 (macrophage elastase)
• matrix metalloproteinase 12 (macrophage elastase)
• Matrix metalloproteinase-12
• ME
• MGC138506
• MME
• MMP12
• MMP-12

Background

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-12 Antibody (NBP1-31225)(5)

Publications using NBP1-31225

• Beres B, Yusenko M, Peterfi L et al. Matrix metalloproteinase 12 is an independent prognostic factor predicting postoperative relapse of conventional renal cell carcinoma - a short report Cellular oncology (Dordrecht) 2021-12-11 [PMID: 34894337] (IF/IHC, Human)
• Garrido-Martin EM, Mellows TWP, Clarke J et al. M1hot tumor-associated macrophages boost tissue-resident memory T cells infiltration and survival in human lung cancer J Immunother Cancer 2020-07-01 [PMID: 32699181]
• Sarlos DP, Banyai D, Peterfi L et al. Embryonal Origin of Metanephric Adenoma and its Differential Diagnosis Anticancer Res. 2018-12-01 [PMID: 30504374] (IF/IHC, Human)
• Damasdi M, Kovacs K, Farkas N et al. Down-regulation of Toll-like Receptor TLR4 Is Associated with HPV DNA Integration in Penile Carcinoma. Anticancer Res. 2017-10-01 [PMID: 28982864] (IF/IHC, Human)
• Janos D VEgstAdiumU vese: modell a progresszIv gyulladAsos mikrokOrnyezet Es tumorgenezis kapcsolatAra Thesis 2022-01-01

Reviews for MMP-12 Antibody (NBP1-31225) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-P Human 02/22/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Human

reviewed by: Verified Customer WB Mouse 06/21/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse adipose tissue homogenate, Sample Amount: 20ug
• Species: Mouse

FAQs for MMP-12 Antibody (NBP1-31225). (Showing 1 - 1 of 1 FAQs).

1. Does this antibody detect the catalytic domain alone of this protein?
   • The immunogen for this protein is between amino acids 213 and 307 of MMP12 (UniProt ID#P39900). This corresponds to a portion of the Hemopexin-like 1 domain on UniProt and does not cover the whole catalytic domain (ZnMc_MMP) which is between amino acids 109 and 263. Please check NCBI Gene ID: 4321 (https://www.ncbi.nlm.nih.gov/gene/?term=4321) for more information.

Bioinformatics

• Gene Symbol: MMP12