Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer ...read more
MMP-12 antibody validated for IHC-P, WB from verified customer reviews.
54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4 using NBP1-31225 in the following applications:
Mouse reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Immunogen affinity purified
Alternate Names for MMP-12 Antibody
matrix metallopeptidase 12 (macrophage elastase)
matrix metalloproteinase 12 (macrophage elastase)
Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for MMP-12 Antibody (NBP1-31225)(5)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: IHC.
FAQs for MMP-12 Antibody (NBP1-31225). (Showing 1 - 1 of 1 FAQs).
Does this antibody detect the catalytic domain alone of this protein?
The immunogen for this protein is between amino acids 213 and 307 of MMP12 (UniProt ID#P39900). This corresponds to a portion of the Hemopexin-like 1 domain on UniProt and does not cover the whole catalytic domain (ZnMc_MMP) which is between amino acids 109 and 263. Please check NCBI 4321 for more information.
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