MMP-12 Antibody

Images

 
Western Blot: MMP-12 Antibody [NBP1-31225] - Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). This image was submitted via customer ...read more
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Paraffin-embedded DLD1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Paraffin-embedded human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MMP-12 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary.
Localization
Secreted, extracellular space, extracellular matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-31225 in the following applications:

Publications
Read Publications using
NBP1-31225 in the following applications:

  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MMP-12 Antibody

  • EC 3.4.24
  • hME
  • HMEEC 3.4.24.65
  • Macrophage elastase
  • macrophage metalloelastase
  • matrix metallopeptidase 12 (macrophage elastase)
  • matrix metalloproteinase 12 (macrophage elastase)
  • Matrix metalloproteinase-12
  • ME
  • MGC138506
  • MME
  • MMP12
  • MMP-12

Background

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85010
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-32398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP1-89559
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85871
Species: Hu
Applications: WB, IHC, IHC-P
MAB9167
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ICC
AF1445
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
MMP200
Species: Hu, Mu, Rt, Po, Ca
DMP300
Species: Hu
NBP2-12553
Species: Hu
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC, IHC-Fr, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB
NB100-2076
Species: Hu, Po, Ca, Fe, Gt, Gp, Rb, Sh
Applications: WB, IHC, IHC-Fr, IHC-P
DMP700
Species: Hu
NBP1-31225
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-12 Antibody (NBP1-31225)(3)

Reviews for MMP-12 Antibody (NBP1-31225) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-31225:
Filter by Applications
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMP-12 NBP1-31225
Enlarge
reviewed by:
Ilya Ulasov
IHC-P Human 02/22/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested breast cancer tissue
SpeciesHuman
Western Blot MMP-12 NBP1-31225
Enlarge
reviewed by:
Kelsey Stockbridge
WB Mouse 06/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse adipose tissue homogenate, Sample Amount: 20ug
SpeciesMouse
CommentsRecognizes 55 kDa proform, 43 kDa activated form and 22 kDa small activated form.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA, 5% fish gelatin,1mM sodium azide in TBST (Ph7.0)

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Dilution Buffer: TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti rabbit from GE healthcare limited, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: pico west, Exposure Time: 1-6 minutes, Specific Bands: 55, 43 and 22 kDa

Comments

CommentsRecognizes 55 kDa proform, 43 kDa activated form and 22 kDa small activated form.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-12 Antibody (NBP1-31225). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody detect the catalytic domain alone of this protein?
    • The immunogen for this protein is between amino acids 213 and 307 of MMP12 (UniProt ID#P39900). This corresponds to a portion of the Hemopexin-like 1 domain on UniProt and does not cover the whole catalytic domain (ZnMc_MMP) which is between amino acids 109 and 263. Please check NCBI 4321 for more information.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional MMP-12 Products

Bioinformatics Tool for MMP-12 Antibody (NBP1-31225)

Discover related pathways, diseases and genes to MMP-12 Antibody (NBP1-31225). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MMP-12 Antibody (NBP1-31225)

Discover more about diseases related to MMP-12 Antibody (NBP1-31225).
 

Pathways for MMP-12 Antibody (NBP1-31225)

View related products by pathway.

PTMs for MMP-12 Antibody (NBP1-31225)

Learn more about PTMs related to MMP-12 Antibody (NBP1-31225).

Blogs on MMP-12.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Ilya Ulasov
02/22/2017
Application: IHC-P
Species: Human

Kelsey Stockbridge
06/21/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MMP12