MED17 Antibody - Azide and BSA Free

Western Blot: MED17 Antibody [NBP3-04651] - Western blot analysis of extracts of various cell lines, using MED17 antibody (NBP3-04651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: MED17 Antibody [NBP3-04651] - Western blot analysis of extracts of various cell lines, using MED17 antibody (NBP3-04651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED17 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MED17 Antibody - Azide and BSA Free (NBP3-04651) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-460 of human MED17 (NP_004259.3). DLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED17
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MED17 Antibody - Azide and BSA Free

• Activator-recruited cofactor 77 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 6
• cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa
• CRSP6FLJ10812
• CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD)
• DRIP77
• DRIP80ARC77
• mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component
• mediator of RNA polymerase II transcription subunit 17
• Thyroid hormone receptor-associated protein complex 80 kDa component
• Transcriptional coactivator CRSP77
• Trap80
• TRAP80CRSP complex subunit 6

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MED17