MED7 Antibody (3E7) - Azide and BSA Free

Western Blot: MED7 Antibody (3E7) [H00009443-M01] - CRSP9 monoclonal antibody (M01), clone 3E7 Analysis of CRSP9 expression in Jurkat.

Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi ( Cat # H00009443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRSP9 monoclonal antibody (M01), clone 3E7 (Cat # H00009443-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 expression in transfected 293T cell line by CRSP9 monoclonal antibody (M01), clone 3E7.Lane 1: CRSP9 transfected lysate(27.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: MED7 Antibody (3E7) [H00009443-M01] - Detection limit for recombinant GST tagged CRSP9 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED7 Antibody (3E7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MED7 Antibody (3E7) - Azide and BSA Free (H00009443-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CRSP9 (AAH05250, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEKDAALCVLIDEMNERP
• Specificity: CRSP9 - cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED7 Antibody (3E7) - Azide and BSA Free

• Activator-recruited cofactor 34 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 9
• cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)
• cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
• CRSP33CRSP complex subunit 9
• CRSP9hMED7
• mediator complex subunit 7ARC34
• mediator of RNA polymerase II transcription subunit 7
• MGC12284
• RNA polymerase transcriptional regulation mediator subunit 7 homolog
• Transcriptional coactivator CRSP33

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED7
• Entrez:
  • 9443(Human)
• OMIM:
  • 605045(Human)