MED12 Antibody


Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 more
Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, more
Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 more
Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody more
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). more
Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 more
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:1,000 (1ug/ml). more

Product Details

Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, IHC, IP, ChIP
1.0 mg/ml

MED12 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).
Predicted Species
Chimpanzee (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31089260).
Theoretical MW
243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-2357 in the following applications:

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for MED12 Antibody

  • ARC240
  • CAG repeat protein 45
  • CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit)
  • KIAA0192FG syndrome 1
  • mediator complex subunit 12OPA-containing protein
  • OKSsubunit 12 homolog
  • thyroid hormone receptor-associated protein, 230 kDa subunit
  • Trap230
  • TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)
  • trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
  • Trinucleotide repeat-containing gene 11 protein


The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Fi, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, RNAi, WB
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IP, KD, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Pm
Applications: IP, WB
Species: Hu, Mu, Rt
Applications: IP, KO, WB
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: ICC, ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, WB
Species: Hu
Applications: ICC, WB

Publications for MED12 Antibody (NB100-2357)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 3 applications: Chemotaxis, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-2357 Applications Species
Huang TW, Iyer AA, Manalo JM et al. Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-07-12 [PMID: 34253626]
Dabral S, Muecke C, Valasarajan C et al. H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas Oncogene 2019-05-15 [PMID: 31089260] (Chemotaxis, Human) Chemotaxis Human
Yokouchi H, Nishihara H, Harada T et al. Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-BRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker Oncotarget 2017-06-13 [PMID: 28055980] (IHC-P, Human) IHC-P Human
Wagner LM, Deluca NA Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription PLoS One 2013-10-11 [PMID: 24147125] (WB, Primate)

Chlorocebus pygerythrus (Vervet monkey)
WB Primate
Vogl MR, Reiprich S, Kuspert M et al. Sox10 Cooperates with the Mediator Subunit 12 during Terminal Differentiation of Myelinating Glia J Neurosci 2013-04-10 [PMID: 23575864] (WB, Human) WB Human
Valdez BC, Nieto Y, Murray D et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol 2012-10-01 [PMID: 22687754]
Miyanari Y, Torres-Padilla ME. Control of ground-state pluripotency by allelic regulation of Nanog. Nature 2012-02-01 [PMID: 22327294]
D'Alessio JA, Ng R, Willenbring H, Tjian R. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A. 2011-02-22 [PMID: 21368148]
Langlais D, Couture C, Sylvain-Drolet G et al. A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells. Mol Endocrinol 2011-02-01 [PMID: 21193556]
Rocha PP, Scholze M, Bleiss W, Schrewe H. Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling Development 2010-08-01 [PMID: 20630950] (WB, Mouse) WB Mouse
Show All 13 Publications.

Reviews for MED12 Antibody (NB100-2357) (0)

There are no reviews for MED12 Antibody (NB100-2357). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MED12 Antibody (NB100-2357). (Showing 1 - 1 of 1 FAQs).

  1. Could you tell me the differences between the two med12 antibodies for the IP? NB100-2357 in this image (image B, IP) you had written "Detection of Human and Mouse MED12 NB100-2357. MED12 was also immunoprecipitated by another rabbit anti-MED12 antibody Which is the other rabbit anti-med12 antibody? Maybe it's more specific for med12 (there is only one band, and no three bands like for NB100-2357).
    • Unfortunately, the other MED12 antibody referred to in the image caption is not available for commercial sale. NB100-2357 displayed a higher affinity for MED12, which can be seen by the stronger signal strength, although it does detect degradation products of MED12, which are the two smaller bands on the blot.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our MED12 Antibody and receive a gift card or discount.


Gene Symbol MED12