Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 ...read more
Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, ...read more
Biological Strategies: Western Blot: MED12 Antibody [NB100-2357] - Detection of Human and Mouse Med12 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis ...read more
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MED12 Antibody [NB100-2357] - Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using ...read more
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31089260).
243 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-2357 in the following applications:
FAQs for MED12 Antibody (NB100-2357). (Showing 1 - 1 of 1 FAQs).
Could you tell me the differences between the two med12 antibodies for the IP? NB100-2357 in this image (image B, IP) you had written "Detection of Human and Mouse MED12 NB100-2357. MED12 was also immunoprecipitated by another rabbit anti-MED12 antibody Which is the other rabbit anti-med12 antibody? Maybe it's more specific for med12 (there is only one band, and no three bands like for NB100-2357).
Unfortunately, the other MED12 antibody referred to in the image caption is not available for commercial sale. NB100-2357 displayed a higher affinity for MED12, which can be seen by the stronger signal strength, although it does detect degradation products of MED12, which are the two smaller bands on the blot.
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