MED12 Antibody

Images

 
Independent Antibodies: Western Blot: MED12 Antibody [NB100-2357] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. ...read more
Biological Strategies: Western Blot: MED12 Antibody [NB100-2357] - Detection of Human and Mouse Med12 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis ...read more
Immunohistochemistry: MED12 Antibody [NB100-2357] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MED12 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-2357 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125). Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MED12 Antibody

  • ARC240
  • CAG repeat protein 45
  • CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit)
  • HOPAFGS1
  • KIAA0192FG syndrome 1
  • mediator complex subunit 12OPA-containing protein
  • OKSsubunit 12 homolog
  • thyroid hormone receptor-associated protein, 230 kDa subunit
  • Trap230
  • TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)
  • trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
  • Trinucleotide repeat-containing gene 11 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MED12 Antibody (NB100-2357)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-2357 Applications Species
Yokouchi H, Nishihara H, Harada T et al. Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-BRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker Oncotarget 2017 Jun 13 [PMID: 28055980] (IHC-P, Human) IHC-P Human
Wagner LM, Deluca NA. et al. Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription. PLoS One. 2013 Oct 11 [PMID: 24147125] (WB, Primate) WB Primate
Vogl MR, Reiprich S, Kuspert M et al. Sox10 Cooperates with the Mediator Subunit 12 during Terminal Differentiation of Myelinating Glia J Neurosci 2013 Apr 10 [PMID: 23575864] (WB, Human) WB Human
Valdez BC, Nieto Y, Murray D et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol 2012 Oct [PMID: 22687754]
Miyanari Y, Torres-Padilla ME. Control of ground-state pluripotency by allelic regulation of Nanog. Nature 2012 Feb [PMID: 22327294]
D'Alessio JA, Ng R, Willenbring H, Tjian R. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A. 2011 Feb 22. [PMID: 21368148]
Langlais D, Couture C, Sylvain-Drolet G et al. A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells. Mol Endocrinol 2011 Feb [PMID: 21193556]
Rocha PP, Scholze M, Bleiss W, Schrewe H. Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling Development 2010 Aug [PMID: 20630950] (WB, Mouse) WB Mouse
Rocha PP, Bleiss W, Schrewe H. Mosaic expression of Med12 in female mice leads to exencephaly, spina bifida, and craniorachischisis. Birth Defects Res A Clin Mol Teratol 2010 Aug [PMID: 20589884] (Mouse) Mouse
Ding N, Tomomori-Sato C, Sato S et al. MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression. J Biol Chem 2009 Jan [PMID: 19049968]
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MED12 Antibody (NB100-2357). (Showing 1 - 1 of 1 FAQs).

  1. Could you tell me the differences between the two med12 antibodies for the IP? NB100-2357 in this image (image B, IP) you had written "Detection of Human and Mouse MED12 NB100-2357. MED12 was also immunoprecipitated by another rabbit anti-MED12 antibody Which is the other rabbit anti-med12 antibody? Maybe it's more specific for med12 (there is only one band, and no three bands like for NB100-2357).
    • Unfortunately, the other MED12 antibody referred to in the image caption is not available for commercial sale. NB100-2357 displayed a higher affinity for MED12, which can be seen by the stronger signal strength, although it does detect degradation products of MED12, which are the two smaller bands on the blot.

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Bioinformatics

Gene Symbol MED12
Entrez
Uniprot