MED12 Antibody

Western Blot: MED12 Antibody [NB100-2357] - Components from all modules of Mediator are found in TAP-wtICP4 samples. Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses at 6 hpi. Results of the Western blot analysis for components of the head (Med6), middle (Med4 and Med1), tail (Med23), kinase (cdk8, Med12, and Med13), and the Med26 protein are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0078242), licensed under a CC-BY licence.

Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, respectively. A. TS = 5, B. TS = 6, C. TS = 6 (x20 original magnification [A, B and C]. Scale bars represent 100 um. TS, total score = intensity score (0-3) plus proportion score (0-3). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/14410), licensed under a CC-BY licence.

Western Blot: MED12 Antibody [NB100-2357] - Detection of Human and Mouse Med12 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody NB100-2357 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: MED12 Antibody [NB100-2357] - Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 antibody NB100-2357 used for IP at 20 ul/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (NBP2-80138). For blotting immunoprecipitated MED12, NBP2-80138 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu, Pm, Pm
• Applications: WB, IHC, IHC-P, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MED12 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31089260).
• Theoretical MW: 243 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MED12 Antibody

• ARC240
• CAG repeat protein 45
• CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit)
• HOPAFGS1
• KIAA0192FG syndrome 1
• mediator complex subunit 12OPA-containing protein
• OKSsubunit 12 homolog
• thyroid hormone receptor-associated protein, 230 kDa subunit
• Trap230
• TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)
• trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
• Trinucleotide repeat-containing gene 11 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED12 Antibody (NB100-2357)(13)

Publications using NB100-2357

• Huang TW, Iyer AA, Manalo JM et al. Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis The Journal of neuroscience : the official journal of the Society for Neuroscience Jul 12 2021 [PMID: 34253626]
• Dabral S, Muecke C, Valasarajan C et al. H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas Oncogene May 15 2019 [PMID: 31089260] (ChIP, Human)
• Yokouchi H, Nishihara H, Harada T et al. Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-BRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker Oncotarget 2017 Jun 13 [PMID: 28055980] (IHC-P, Human)
• Wagner LM, Deluca NA. et al. Temporal Association of Herpes Simplex Virus ICP4 with Cellular Complexes Functioning at Multiple Steps in PolII Transcription. PLoS One. 2013 Oct 11 [PMID: 24147125] (WB, Primate)
• Vogl MR, Reiprich S, Kuspert M et al. Sox10 Cooperates with the Mediator Subunit 12 during Terminal Differentiation of Myelinating Glia J Neurosci 2013 Apr 10 [PMID: 23575864] (WB, Human)
• Valdez BC, Nieto Y, Murray D et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol 2012 Oct [PMID: 22687754]
• Miyanari Y, Torres-Padilla ME. Control of ground-state pluripotency by allelic regulation of Nanog. Nature 2012 Feb [PMID: 22327294]
• D'Alessio JA, Ng R, Willenbring H, Tjian R. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A. 2011 Feb 22. [PMID: 21368148]
• Langlais D, Couture C, Sylvain-Drolet G et al. A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells. Mol Endocrinol 2011 Feb [PMID: 21193556]
• Rocha PP, Scholze M, Bleiss W, Schrewe H. Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling Development 2010 Aug [PMID: 20630950] (WB, Mouse)
• Rocha PP, Bleiss W, Schrewe H. Mosaic expression of Med12 in female mice leads to exencephaly, spina bifida, and craniorachischisis. Birth Defects Res A Clin Mol Teratol 2010 Aug [PMID: 20589884] (Mouse)
• Ding N, Tomomori-Sato C, Sato S et al. MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression. J Biol Chem 2009 Jan [PMID: 19049968]
• Tutter AV, Kowalski MP, Baltus GA et al. Role for Med12 in regulation of Nanog and Nanog target genes. J Biol Chem 2009 Feb [PMID: 19036726]

FAQs for MED12 Antibody (NB100-2357). (Showing 1 - 1 of 1 FAQs).

1. Could you tell me the differences between the two med12 antibodies for the IP? NB100-2357 in this image (image B, IP) you had written "Detection of Human and Mouse MED12 NB100-2357. MED12 was also immunoprecipitated by another rabbit anti-MED12 antibody Which is the other rabbit anti-med12 antibody? Maybe it's more specific for med12 (there is only one band, and no three bands like for NB100-2357).
   • Unfortunately, the other MED12 antibody referred to in the image caption is not available for commercial sale. NB100-2357 displayed a higher affinity for MED12, which can be seen by the stronger signal strength, although it does detect degradation products of MED12, which are the two smaller bands on the blot.

Bioinformatics

• Gene Symbol: MED12
• Entrez:
  • 9968(Human)
  • 59024(Mouse)
• Uniprot:
  • Q93074()