TRACP/PAP/ACP5 Antibody (SPM601)

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Immunohistochemistry-Paraffin: TRACP/PAP/ACP5 Antibody (SPM601) [NBP2-45294] - Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (SPM601).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
SPM601
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

TRACP/PAP/ACP5 Antibody (SPM601) Summary

Immunogen
Recombinant full-length human TRACP/PAP/ACP5 protein
Localization
Cytoplasmic (Lysosomes)
Marker
Hairy Cell Leukemia Marker
Specificity
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACP5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-45294.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP3-11536).

Alternate Names for TRACP/PAP/ACP5 Antibody (SPM601)

  • ACP5
  • HPAP
  • serum band 5 tartrate-resistant acid phosphatase
  • SPENCDI
  • TRACP
  • TRACP5a
  • TRACP5b
  • TRACP-5b
  • TRAP
  • TrATPase
  • TTRRAP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRACP/PAP/ACP5 Antibody (NBP2-45294)(1)

We have publications tested in 1 confirmed species: Rat.


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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ACP5