TRAP220/MED1 Antibody

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP1/TRAP220 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit antiCRSP1/TRAP220 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1(TRAP220) on HeLa whole cell lysate using NB100-2574.

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CRSP1/TRAP220 antibody NB100-2574 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit antiCRSP1/TRAP220 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TRAP220/MED1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1using the numbering given in entry NP_004765.2 (GeneID 5469).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000 -1:10000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 -5 ug/mg lysate
  • Proximity Ligation Assay 1:1000 - 1:20000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Positive Control:

  HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAP220/MED1 Antibody

• Activator-recruited cofactor 205 kDa component
• ARC205
• CRSP1TRIP2
• CRSP200p53 regulatory protein RB18A
• DRIP230
• DRIP230PPAR-binding protein
• MBD4
• MED1
• mediator complex subunit 1DRIP205
• mediator of RNA polymerase II transcription subunit 1
• PBPPPARGBP
• Peroxisome proliferator-activated receptor-binding protein
• PPAR binding protein
• PPARBP
• PPARBPMGC71488
• PPARG binding protein
• PPARGBP
• RB18ATR-interacting protein 2
• Thyroid hormone receptor-associated protein complex 220 kDa component
• thyroid hormone receptor-associated protein complex component TRAP220
• thyroid receptor interacting protein 2
• Thyroid receptor-interacting protein 2
• TRAP220
• TRAP220TRIP-2
• TRIP2
• vitamin D receptor-interacting protein 230 kD
• Vitamin D receptor-interacting protein complex component DRIP205

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED1
• Entrez:
  • 5469(Human)
  • 19014(Mouse)
  • 497991(Rat)
• Uniprot:
  • NP_004765.2(Human)