TRAP220/MED1 Antibody

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of10 seconds.

Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:1000 (1ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG.

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1(TRAP220) on HeLa whole cell lysate using NB100-2574.

Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574] - Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED1 antibody NB100-2574(lot NB100-2574-9) used for IP at 6 ug per reaction. For blotting immunoprecipitated MED1, NB100-2574 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 30 seconds.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IHC-P, IP, IHC-Fr (-), KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TRAP220/MED1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1using the numbering given in entry NP_004765.2 (GeneID 5469).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000 -1:10000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2 -5 ug/mg lysate
  • Knockdown Validated
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Knockdown validation (PMID: 31695025).
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAP220/MED1 Antibody

• Activator-recruited cofactor 205 kDa component
• ARC205
• CRSP1TRIP2
• CRSP200p53 regulatory protein RB18A
• DRIP230
• DRIP230PPAR-binding protein
• MBD4
• MED1
• mediator complex subunit 1DRIP205
• mediator of RNA polymerase II transcription subunit 1
• PBPPPARGBP
• Peroxisome proliferator-activated receptor-binding protein
• PPAR binding protein
• PPARBP
• PPARBPMGC71488
• PPARG binding protein
• PPARGBP
• RB18ATR-interacting protein 2
• Thyroid hormone receptor-associated protein complex 220 kDa component
• thyroid hormone receptor-associated protein complex component TRAP220
• thyroid receptor interacting protein 2
• Thyroid receptor-interacting protein 2
• TRAP220
• TRAP220TRIP-2
• TRIP2
• vitamin D receptor-interacting protein 230 kD
• Vitamin D receptor-interacting protein complex component DRIP205

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAP220/MED1 Antibody (NB100-2574)(1)

Publication using NB100-2574

• BrUckmann NH, Bennedsen SN, Duijf PHG et al. A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence Cell Death Dis Nov 6 2019 [PMID: 31695025] (IHC, KD, Human)

Bioinformatics

• Gene Symbol: MED1
• Entrez:
  • 5469(Human)
  • 19014(Mouse)
• Uniprot:
  • NP_004765.2(Human)