Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibodyused for WB ...read more
Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:1000 (1ug/ml). Secondary: HRP-conjugated goat ...read more
Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1(TRAP220) on HeLa whole cell lysate using NB100-2574.
Independent Antibodies: Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574] - Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP ...read more
The immunogen recognized by this antibody maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1using the numbering given in entry NP_004765.2 (GeneID 5469).
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Immunogen affinity purified
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mediator of RNA polymerase II transcription subunit 1
Peroxisome proliferator-activated receptor-binding protein
PPAR binding protein
PPARG binding protein
RB18ATR-interacting protein 2
Thyroid hormone receptor-associated protein complex 220 kDa component
thyroid hormone receptor-associated protein complex component TRAP220
thyroid receptor interacting protein 2
Thyroid receptor-interacting protein 2
vitamin D receptor-interacting protein 230 kD
Vitamin D receptor-interacting protein complex component DRIP205
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.