TRAP220/MED1 Antibody

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Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used for WB at 0.1 ...read more
Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:5000 (0.2ug/ml). Secondary: HRP-conjugated goat ...read more
Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (10 ug) from RKO, HeLa, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used ...read more
Independent Antibodies: Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574] - Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm-OrSpecies Glossary
Applications WB, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

TRAP220/MED1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1using the numbering given in entry NP_004765.2 (GeneID 5469).
Predicted Species
Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 -10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000 -1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Knockdown validation (PMID: 31695025).
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-2574 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAP220/MED1 Antibody

  • Activator-recruited cofactor 205 kDa component
  • ARC205
  • CRSP1TRIP2
  • CRSP200p53 regulatory protein RB18A
  • DRIP230
  • DRIP230PPAR-binding protein
  • MBD4
  • MED1
  • mediator complex subunit 1DRIP205
  • mediator of RNA polymerase II transcription subunit 1
  • PBPPPARGBP
  • Peroxisome proliferator-activated receptor-binding protein
  • PPAR binding protein
  • PPARBP
  • PPARBPMGC71488
  • PPARG binding protein
  • PPARGBP
  • RB18ATR-interacting protein 2
  • Thyroid hormone receptor-associated protein complex 220 kDa component
  • thyroid hormone receptor-associated protein complex component TRAP220
  • thyroid receptor interacting protein 2
  • Thyroid receptor-interacting protein 2
  • TRAP220
  • TRAP220TRIP-2
  • TRIP2
  • vitamin D receptor-interacting protein 230 kD
  • Vitamin D receptor-interacting protein complex component DRIP205

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAP220/MED1 Antibody (NB100-2574)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MED1
Entrez
Uniprot