Transgelin/TAGLN/SM22 alpha Antibody

Images

 
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of human placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Transgelin/TAGLN/SM22 alpha Antibody Summary

Immunogen
Peptide with sequence C-MTGYGRPRQIIS corresponding to C-Terminus according to NP_003177.2.
Specificity
NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumor suppressor.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01-0.03 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 24 kDa band observed in human placenta lysates (calculated MW of 22.6 kDa band according to NP_003177.2). This antibody has been successfully used in Western blot in mouse (PMID: PMID: 25457224, 20089836) and human (PMID: 21629665, PMID: 19337690).
Theoretical MW
22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Transgelin/TAGLN/SM22 alpha Lysate (NBL1-16691)
Reviewed Applications
Read 1 Review rated 5
using
NB600-507 in the following applications:

Publications
Read Publications using
NB600-507 in the following applications:

  • WB
    11 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-47560
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15141
Species: Hu
Applications: WB
NBP1-80616
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-13387
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84745
Species: Hu
Applications: IHC, IHC-P
NBP1-33063
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF6897
Species: Hu, Mu
Applications: WB, IHC
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
MAB4028
Species: Hu, Mu
Applications: WB
NBP2-45731
Species: Hu, Ca, Mk
Applications: WB, IHC, IHC-P

Publications for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)(19)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 1 application: WB.


Filter By Application
WB
(11)
All Applications
Filter By Species
Human
(3)
Rat
(6)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB600-507 Applications Species
Hong K, Zhao G, Hong Z et al. Mechanical activation of angiotensin II type 1 receptors causes actin remodelling and myogenic responsiveness in skeletal muscle arterioles. J. Physiol. (Lond.). Aug 17 2016 [PMID: 27531064] (WB, Rat) WB Rat
Herencia C, Diaz-Tocados JM, Jurado L et al. Procaine Inhibits Osteo/Odontogenesis through Wnt/b-Catenin Inactivation. PLoS ONE Jun 4 2016 [PMID: 27257912]
Thibodeau JF, Holterman CE, He Y et al. Vascular smooth muscle-specific EP4 receptor deletion in mice exacerbates angiotensin II-induced renal injury. Antioxid. Redox Signal. Jun 1 2016 [PMID: 27245461]
Abd-Elrahman KS, Colinas O, Walsh EJ et al. Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat. J. Cereb. Blood Flow Metab. 2015 Dec 31 [PMID: 26721393] (WB, Rat) WB Rat
Colinas O, Moreno-Dominguez A, Zhu HL et al. alpha5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries. Biochem. Pharmacol. 2015 Aug 14 [PMID: 26278977] (WB, Rat) WB Rat
El-Yazbi AF, Abd-Elrahman KS, Moreno-Dominguez A. PKC-mediated cerebral vasoconstriction: role of myosin light chain phosphorylation versus actin cytoskeleton reorganization Biochem. Pharmacol. 2015 Apr 27 [PMID: 25931148] (WB, Rat) WB Rat
Deng JT, Wang XL, Chen YX et al. The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells PLoS ONE 2015 Feb 28 [PMID: 25723491] (WB, Human)

Details:
goat anti-SM-22 antibody used at 1:2500 dilution in WB application for analysis of Transgelin/TAGLN/SM22 alpha protein in lysates of control human Umbilical Artery Smooth Muscle Cells/UASMCs and UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. SM22 in this assay was used as loading control for the quantitative analysis of IL1 Beta protein (Fig 4A).
WB Human
Hovater MB, Ying WZ, Agarwal A, Sanders PW. Nitric oxide and carbon monoxide antagonize TGF-b through ligand-independent internalization of TbR1/ALK5. Am. J Physiol. Renal Physiol. 2014 Aug 06 [PMID: 25100282] (WB) WB
Moreno-Dominguez A, El-Yazbi AF, Zhu HL et al. Cytoskeletal Reorganization Evoked by Rho-associated kinase- and Protein Kinase C-Catalyzed Phosphorylation of Cofilin and Heat Shock Protein 27, Respectively, Contributes to Myogenic Constriction of Rat Cerebral Arteries. J. Biol. Chem. 2014 Jun 09 [PMID: 24914207] (WB, Rat)

Details:
Antibody used for WB on RMCAs/rat middle cerebral arteries (Sprague-Dawley rats) - goat polyclonal anti-SM22 alpha (1:2000 dilution), incubated overnight at 4 C in 1% dry milk in 0.1% TBST (article has highly detailed WB method under G-actin Determination section); WB images -FIGURES 1E, 2E, 3E, 4C, 5C, 7A/B, 8A, 13A - Levels of G-actin were normalized to SM22 content, because SM22 is exclusively retained in the supernatant during high speed centrifugation and expressed as a fraction of the level at 10 mm Hg (Walshet al. 2011 Am. J. Physiol. Renal Physiol. 300, F73–F85)
WB Rat
Ying WZ, Aaron KJ, Sanders PW. Transforming Growth Factor-beta Regulates Endothelial Function During High Salt Intake in Rats. Hypertension. 2013 Nov [PMID: 24041947] (WB, Human) WB Human
Show All 19 Publications.

Review for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB600-507:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Transgelin/TAGLN/SM22 alpha NB600-507
Enlarge
reviewed by:
Katie Tobik
IF Human 11/03/2017
View

Summary

ApplicationImmunofluorescence
Sample Testedadipose derived stem cells differentiated to smooth muscle cells
SpeciesHuman
LotC3G2 E160117

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507). (Showing 1 - 1 of 1 FAQs).

  1. We are using the Transgelin antibody (NB600-507) for western analysis of mouse and human uterine tissue. In the mouse we see a single band of 22 kDa but in the human we see a band of 12 kDa. Any thoughts on this? I can not find any information on a smaller transgelin band.
    • I have searched for an explanation for the lower molecular weight band you are detecting, and have found that this paper, PMC2789179 says: 'It should be noted that the expression analysis of TAGLN is complicated by the fact that multiple bands were detected on the Western blots. Similar observations have been described and attributed to C-terminal proteolysis. While the sum of band intensities was used for the analyses here, it is possible that TAGLN regulation is not a simple change in quantity of one defined species, but may, in fact, be subject to posttranslationally regulated events such as modification or degradation.'

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Transgelin/TAGLN/SM22 alpha Products

Bioinformatics Tool for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)

Discover related pathways, diseases and genes to Transgelin/TAGLN/SM22 alpha Antibody (NB600-507). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)

Discover more about diseases related to Transgelin/TAGLN/SM22 alpha Antibody (NB600-507).
 

Pathways for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)

View related products by pathway.

PTMs for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)

Learn more about PTMs related to Transgelin/TAGLN/SM22 alpha Antibody (NB600-507).

Blogs on Transgelin/TAGLN/SM22 alpha

There are no specific blogs for Transgelin/TAGLN/SM22 alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Katie Tobik
11/03/2017
Application: IF
Species: Human

Bioinformatics

Gene Symbol TAGLN
Entrez
Uniprot