Peptide with sequence C-MTGYGRPRQIIS corresponding to C-Terminus according to NP_003177.2.
NP_003177.2 and NP_001001522.1 represent identical protein.
The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.
Immunogen affinity purified
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WB: Approx. 24 kDa band observed in human placenta lysates (calculated MW of 22.6 kDa band according to NP_003177.2).
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Canine, Bovine.
Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody
SM2222 kDa actin-binding protein
Smooth muscle protein 22-alpha
transgelin variant 2
In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507). (Showing 1 - 1 of 1 FAQs).
We are using the Transgelin antibody (NB600-507) for western analysis of mouse and human uterine tissue. In the mouse we see a single band of 22 kDa but in the human we see a band of 12 kDa. Any thoughts on this? I can not find any information on a smaller transgelin band.
I have searched for an explanation for the lower molecular weight band you are detecting, and have found that this paper, PMC2789179 says: 'It should be noted that the expression analysis of TAGLN is complicated by the fact that multiple bands were detected on the Western blots. Similar observations have been described and attributed to C-terminal proteolysis. While the sum of band intensities was used for the analyses here, it is possible that TAGLN regulation is not a simple change in quantity of one defined species, but may, in fact, be subject to posttranslationally regulated events such as modification or degradation.'