Transgelin/TAGLN/SM22 alpha Antibody

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Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, Bv, CaSpecies Glossary
Applications WB, IHC, PEP-ELISA, KD
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Transgelin/TAGLN/SM22 alpha Antibody Summary

Immunogen
Peptide with sequence C-MTGYGRPRQIIS corresponding to C-Terminus according to NP_003177.2.
Specificity
NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumor suppressor.
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/mL
  • Immunohistochemistry
  • Peptide ELISA 1:4000
  • Knockdown Validated
Application Notes
Western blot: Approx 23-24 kDa band observed in Human Testis lysates (calculated MW of 22.6 kDa according to NP_003177.2 and NP_001001522.1). Preliminary testing also showed a band at approx 23 kDa in lysates of cell line HeLa and approx 25 kDa in Human Breast and Placenta lysates.Use in Immunohistochemistry reported in scientific literature (PMID:31965901). Knockdown validation (PMID: 32134397).
Control
HeLa Whole Cell Lysate
Transgelin/TAGLN/SM22 alpha Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-507 in the following applications:

Publications
Read Publications using
NB600-507 in the following applications:

  • IHC
    1 publication
  • KD
    1 publication
  • WB
    14 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine. This antibody has been successfully used in Western blot in mouse (PMID: PMID: 25457224, 20089836) and human (PMID: 21629665, PMID: 19337690), Rat (PMID: 27531064), Rabbit (PMID: 20861082).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 3 applications: IHC, KD, WB.


Filter By Application
IHC
(1)
KD
(1)
WB
(14)
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Human
(4)
Mouse
(1)
Rat
(6)
Rabbit
(1)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB600-507 Applications Species
Li J, Liu H, Takagi S et Al. Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules JCI Insight Mar 26 2020 [PMID: 32134397] (WB, KD, Mouse) WB, KD Mouse
Takagaki Y, Lee SM, Dongqing Z et al. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis Autophagy Jan 22 2020 [PMID: 31965901] (IHC, WB) IHC, WB
Deng JT, Bhaidani S, Sutherland C Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells PLoS ONE Dec 13 2019 [PMID: 31834925] (WB, Human) WB Human
Hong K, Zhao G, Hong Z et al. Mechanical activation of angiotensin II type 1 receptors causes actin remodelling and myogenic responsiveness in skeletal muscle arterioles. J. Physiol. (Lond.). Aug 17 2016 [PMID: 27531064] (WB, Rat) WB Rat
Herencia C, Diaz-Tocados JM, Jurado L et al. Procaine Inhibits Osteo/Odontogenesis through Wnt/b-Catenin Inactivation. PLoS ONE Jun 4 2016 [PMID: 27257912]
Thibodeau JF, Holterman CE, He Y et al. Vascular smooth muscle-specific EP4 receptor deletion in mice exacerbates angiotensin II-induced renal injury. Antioxid. Redox Signal. Jun 1 2016 [PMID: 27245461]
Abd-Elrahman KS, Colinas O, Walsh EJ et al. Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat. J. Cereb. Blood Flow Metab. 2015 Dec 31 [PMID: 26721393] (WB, Rat) WB Rat
Colinas O, Moreno-Dominguez A, Zhu HL et al. alpha5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries. Biochem. Pharmacol. 2015 Aug 14 [PMID: 26278977] (WB, Rat) WB Rat
El-Yazbi AF, Abd-Elrahman KS, Moreno-Dominguez A. PKC-mediated cerebral vasoconstriction: role of myosin light chain phosphorylation versus actin cytoskeleton reorganization Biochem. Pharmacol. 2015 Apr 27 [PMID: 25931148] (WB, Rat) WB Rat
Deng JT, Wang XL, Chen YX et al. The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells PLoS ONE 2015 Feb 28 [PMID: 25723491] (WB, Human)

Details:
goat anti-SM-22 antibody used at 1:2500 dilution in WB application for analysis of Transgelin/TAGLN/SM22 alpha protein in lysates of control human Umbilical Artery Smooth Muscle Cells/UASMCs and UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. SM22 in this assay was used as loading control for the quantitative analysis of IL1 Beta protein (Fig 4A).
WB Human
Show All 22 Publications.

Review for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-507:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Transgelin/TAGLN/SM22 alpha NB600-507
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reviewed by:
Katie Tobik
IF Human 11/03/2017
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Summary

ApplicationImmunofluorescence
Sample Testedadipose derived stem cells differentiated to smooth muscle cells
SpeciesHuman
LotC3G2 E160117

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FAQs for Transgelin/TAGLN/SM22 alpha Antibody (NB600-507). (Showing 1 - 1 of 1 FAQs).

  1. We are using the Transgelin antibody (NB600-507) for western analysis of mouse and human uterine tissue. In the mouse we see a single band of 22 kDa but in the human we see a band of 12 kDa. Any thoughts on this? I can not find any information on a smaller transgelin band.
    • I have searched for an explanation for the lower molecular weight band you are detecting, and have found that this paper, PMC2789179 says: 'It should be noted that the expression analysis of TAGLN is complicated by the fact that multiple bands were detected on the Western blots. Similar observations have been described and attributed to C-terminal proteolysis. While the sum of band intensities was used for the analyses here, it is possible that TAGLN regulation is not a simple change in quantity of one defined species, but may, in fact, be subject to posttranslationally regulated events such as modification or degradation.'

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Recent Reviews

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Katie Tobik
11/03/2017
Application: IF
Species: Human

Bioinformatics

Gene Symbol TAGLN
Entrez
Uniprot