MBD4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: MBD4 Antibody [NBP1-91189] - IHC analysis of MBD4 in human breast cancer cells using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.11 mg/ml

MBD4 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human MBD4 protein (between residues 200-250) [UniProt O95243]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MBD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This MBD4 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.
Control
MBD4 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP1-91189 in the following applications:

Reactivity Notes

Human. Does not react with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.11 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MBD4 Antibody

  • EC 3.2.2.-
  • G/T mismatch glycosylase
  • G/U mismatch glycosylase
  • MBD4
  • MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics
  • methyl-CpG binding domain protein 4
  • methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase
  • Methyl-CpG-binding endonuclease 1
  • Methyl-CpG-binding protein MBD4
  • Mismatch-specific DNA N-glycosylase
  • putative methyl-CpG binding protein

Background

MBD4, methyl-CpG binding domain protein 4 (MED1), is part of the mammalian DNA glycosylase superfamily. MBD4 may be involved in maintaining genome stability, epigenetics via DNA methylation, as well as carcinogenesis. MBD4 has an amino-proximal methyl-CpG binding domain and a C-terminal mismatch-specific glycosylase domain. This configuration is thought to help maintain genome stability (PMID: 21820404). Proteins with methylcytosine-binding domains act as transcriptional repressors via promoter methylation, as well as promote the gene-silencing effect of DNA methylation. A study of patients with primary immune thrombocytopenia has shown that a decrease in expression of MBD4 may be involved with the pathogenesis of the disease (PMID: 21377502). Additionally, MBD4 has been shown to be a human DNA repair protein that is possibly involved in DNA mismatch repair. Furthermore, a study investigating microsatellite instability found that the MBD4 codon 346 polymorphism may be a factor in colorectal cancer susceptibility in the Korean population (PMID: 19469655).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBD4 Antibody (NBP1-91189)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


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ICC/IF
(1)
IHC
(1)
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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for MBD4 Antibody (NBP1-91189) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-91189AF405
Alexa Fluor 488 NBP1-91189AF488
Alexa Fluor 647 NBP1-91189AF647
Alexa Fluor 700 NBP1-91189AF700
Biotin NBP1-91189B
DyLight 405 NBP1-91189V
DyLight 488 NBP1-91189G
DyLight 550 NBP1-91189R
DyLight 650 NBP1-91189C
FITC NBP1-91189F
HRP NBP1-91189H
Janelia Fluor 549 NBP1-91189JF549
Janelia Fluor 646 NBP1-91189JF646

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Bioinformatics

Gene Symbol MBD4
Entrez
Uniprot