MBD4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: MBD4 Antibody [NBP1-91189] - IHC analysis of MBD4 in human breast cancer cells using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu(-)
• Applications: ICC/IF, IHC, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.11 mg/ml

MBD4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MBD4 Antibody - BSA Free (NBP1-91189) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-MBD4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of the human MBD4 protein (between residues 200-250) [UniProt O95243]
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: This MBD4 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.

Reactivity Notes

Does not react with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MBD4 Antibody - BSA Free

• EC 3.2.2.-
• G/T mismatch glycosylase
• G/U mismatch glycosylase
• MBD4
• MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• methyl-CpG binding domain protein 4
• methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase
• Methyl-CpG-binding endonuclease 1
• Methyl-CpG-binding protein MBD4
• Mismatch-specific DNA N-glycosylase
• putative methyl-CpG binding protein

Background

MBD4, methyl-CpG binding domain protein 4 (MED1), is part of the mammalian DNA glycosylase superfamily. MBD4 may be involved in maintaining genome stability, epigenetics via DNA methylation, as well as carcinogenesis. MBD4 has an amino-proximal methyl-CpG binding domain and a C-terminal mismatch-specific glycosylase domain. This configuration is thought to help maintain genome stability (PMID: 21820404). Proteins with methylcytosine-binding domains act as transcriptional repressors via promoter methylation, as well as promote the gene-silencing effect of DNA methylation. A study of patients with primary immune thrombocytopenia has shown that a decrease in expression of MBD4 may be involved with the pathogenesis of the disease (PMID: 21377502). Additionally, MBD4 has been shown to be a human DNA repair protein that is possibly involved in DNA mismatch repair. Furthermore, a study investigating microsatellite instability found that the MBD4 codon 346 polymorphism may be a factor in colorectal cancer susceptibility in the Korean population (PMID: 19469655).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBD4 Antibody (NBP1-91189)(1)

Publication using NBP1-91189

• Kristensen DG, Nielsen JE, Jorgensen A et al. Evidence that active demethylation mechanisms maintain the genome of carcinoma in situ cells hypomethylated in the adult testis. Br J Cancer. 2013-11-28 [PMID: 24292451] (ICC/IF, IF/IHC, Human)

Bioinformatics

• Gene Symbol: MBD4
• Entrez:
  • 8930(Human)
• Uniprot:
  • O95243(Human)