MED24 Antibody - BSA Free

Western Blot: MED24 Antibody [NB100-74599] - Detection of Human and Mouse TRAP100/MED24 on HeLa whole cell lysate using NB100-74599.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MED24 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 939 and 989 of human thyroid hormone receptor-associated protein complex 100 kDa component (mediator complex subunit 24 ) using the numbering given in entry NP_055630.2 (GeneID 986).
• Localization: Nuclear
• Predicted Species: Rat (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MED24 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MED24 Antibody - BSA Free

• cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa
• CRSP100
• DRIP100CRSP complex subunit 4
• hTRAP100
• KIAA0130CRSP4
• mediator complex subunit 24ARC100
• MGC8748
• subunit 24 homolog
• thyroid hormone receptor associated protein 4
• Thyroid hormone receptor-associated protein 4
• Thyroid hormone receptor-associated protein complex 100 kDa component
• Trap100
• TRAP100THRAP4
• Vitamin D3 receptor-interacting protein complex 100 kDa component
• vitamin D3 receptor-interacting protein complex component DRIP100

Background

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED24 Antibody (NB100-74599)(1)

Publication using NB100-74599

• Chen X, Zhao J, Gu C et al. Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells. Nat Commun 2018-09-14 [PMID: 30218073] (WB, Mouse)

Bioinformatics

• Gene Symbol: MED24
• Entrez:
  • 9862(Human)
  • 23989(Mouse)
• Uniprot:
  • O75448(Human)