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SLC16A3 Antibody

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 (1 Review)
 
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Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human lung shows strong membranous positivity in macrophages.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Specificity
Specificity of human SLC16A3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:250
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
Use in WB reported in scientific literature (PMID:33212210) IHC-FR reactivity reported in (PMID:24692237), IB reactivity reported in (PMID: 28978054).
Control
SLC16A3 Knockout HeLa Cell Lysate
Control Peptide
SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:
Publications
Read Publications using
NBP1-81251 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-59656
Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.
Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.
Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.
(5 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MCTS1 Antibody
(OTI2F9)
NBP2-03626
Western Blot: MCT1 Antibody (2F9) [NBP2-03626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCT1.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] Staining of paraffin-embedded Human tonsil using anti-MCT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCT1 mouse monoclonal antibody.
Species: Hu
Applications: WB, IHC, IHC-P
Chymase/CMA1/Mast
Cell Chymase Antibody ...
MAB4099
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Reviews)
(1 Publication)
Species: Hu
Applications: WB, IP
MCAT Antibody (2F2)
H00027349-M01
Western Blot: MCAT Antibody (2F2) [H00027349-M01] - MT monoclonal antibody (M01), clone 2F2 Analysis of MT expression in HeLa.
Immunohistochemistry-Paraffin: MCAT Antibody (2F2) [H00027349-M01] - Analysis of monoclonal antibody to MT on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: MCAT Antibody (2F2) [H00027349-M01] - Detection limit for recombinant GST tagged MT is approximately 1ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
MCPH1 Antibody
AF3998
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
(4 Publications)
Species: Hu
Applications: WB
MCT2 Antibody
NB300-877
Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877] - Staining of Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(8 Publications)
Species: Hu
Applications: WB, Simple Western, ELISA, IHC, KO
SLC16A4 Antibody
NBP2-13315
Immunocytochemistry/Immunofluorescence: SLC16A4 Antibody [NBP2-13315] - Staining of human cell line BJ shows localization to microtubules. Antibody staining is shown in green.
Immunohistochemistry: SLC16A4 Antibody [NBP2-13315] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: SLC16A4 Antibody [NBP2-13315] - Staining of human placenta shows moderate membranous positivity in trohphoblastic cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Glut1 Antibody
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY licence.
Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.
(7 Reviews)
(40 Publications)
Species: Hu, Mu, Rt, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Flow-IC
CS Citrate
Synthase Antibody (17
NBP2-43648
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (Negative) and transfected (Positive) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
RPE Antibody
NBP1-56853
Western Blot: RPE Antibody [NBP1-56853] - Human Muscle lysate, concentration 0.2-1 ug/ml.
Immunocytochemistry/Immunofluorescence: RPE Antibody [NBP1-56853] - Antibody 0 Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm but only in connective tissue cells in the interlobular septum, very low tissue distribution Primary Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MCT8/SLC16A2
Antibody
NBP2-57308
Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Glut4 Antibody
NBP1-49533
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
(4 Reviews)
(19 Publications)
Species: Hu, Mu, Rt, Pl
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC, KD
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(22 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.
Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.
(43 Reviews)
(876 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, TCS, IHC-FrFl, KD, KO, LA
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu
SLC16A10 Antibody
NBP1-80700
Immunocytochemistry/Immunofluorescence: SLC16A10 Antibody [NBP1-80700] - Staining of human cell line CACO-2 shows localization to cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A10 Antibody [NBP1-80700] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SLC16A6 Antibody
NBP1-59881
Western Blot: SLC16A6 Antibody [NBP1-59881] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
SLC16A3 Antibody
NBP1-81251
SLC16A3 Antibody
Species: Hu, Mu
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for SLC16A3 Antibody (NBP1-81251)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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Review for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-81251:
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(1)
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Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
Enlarge 		5
reviewed by:
Anonymous
 WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

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WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQs).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Control Lysate(s)

SLC16A3 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Bioinformatics Tool for SLC16A3 Antibody (NBP1-81251)

Discover related pathways, diseases and genes to SLC16A3 Antibody (NBP1-81251). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Western Blot SLC16A3 NBP1-81251
Enlarge
 Anonymous
06/04/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot