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SLC16A3 Antibody

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Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human lung shows strong membranous positivity in macrophages.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Specificity
Specificity of human SLC16A3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24692237).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
SLC16A3 Lysate (NBP2-64667)
Control Peptide
SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:
Publications
Read Publications using
NBP1-81251 in the following applications:

Reactivity Notes

Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.
Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.
Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.
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Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MCTS1 Antibody
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Western Blot: MCT1 Antibody (2F9) [NBP2-03626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCT1.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] Staining of paraffin-embedded Human tonsil using anti-MCT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCT1 mouse monoclonal antibody.
Species: Hu
Applications: WB, IHC, IHC-P
Chymase/CMA1/Mast
Cell Chymase Antibody
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Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human esophagus shows high expression.
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining in human esophagus and cerebral cortex tissues using anti-CMA1 antibody. Corresponding CMA1 RNA-seq data are presented for the same tissues.
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Species: Hu
Applications: ICC/IF, IHC, IHC-P
MCAT Antibody
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Western Blot: MCAT Antibody [NBP1-83936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MCAT Antibody [NBP1-83936] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Species: Hu
Applications: WB, IHC, IHC-P
MCPH1 Antibody
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
(4 Publications)
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Applications: WB
MCT2 Antibody
NB300-877
Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
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Applications: WB, IHC-P, PEP-ELISA
EMMPRIN/CD147
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NB100-64848
Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Analysis of FFPE tissue section of rat kidney using 1:100 dilution of CD147 antibody (clone OX-47). The staining was detected using HRP-labelled secondary antibody and DAB reagent which followed counterstaining of sections with hematoxylin. This antibody generated primarily a membrane staining with some cytoplasmic signal in the cuboidal epithelial cells of various tubules.
(6 Publications)
Species: Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
SLC16A4 Antibody
NBP2-13315
Immunocytochemistry/Immunofluorescence: SLC16A4 Antibody [NBP2-13315] - Immunofluorescent staining of human cell line BJ shows localization to microtubules.
Immunohistochemistry-Paraffin: SLC16A4 Antibody [NBP2-13315] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Glut1 Antibody
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - (PMID: 21620138 ).
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.
(6 Reviews)
(21 Publications)
Species: Hu, Mu, Rt, Rb
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
CS Citrate
Synthase Antibody
NBP2-13878
Western Blot: CS Citrate Synthase Antibody [NBP2-13878] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody [NBP2-13878] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody [NBP2-13878] - Staining of human pancreas.
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
RPE Antibody
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Western Blot: RPE Antibody [NBP1-56853] - Human Muscle lysate, concentration 0.2-1 ug/ml.
Immunocytochemistry/Immunofluorescence: RPE Antibody [NBP1-56853] - Antibody 0 Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm but only in connective tissue cells in the interlobular septum, very low tissue distribution Primary Antibody
Species: Hu
Applications: WB, ICC/IF, IHC
MCT8/SLC16A2
Antibody
NBP2-57308
Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
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H00009121-R01
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Glut4 Antibody
NBP1-49533
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
(3 Reviews)
(8 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NOD2 Antibody (2D9)
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(14 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.
Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
(33 Reviews)
(751 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
HLA DQA1 Antibody
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Species: Hu
Applications: WB, IHC, IHC-P
CTLA-4
[Unconjugated]
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Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(2 Reviews)
(1 Publication)
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SLC16A10 Antibody
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Immunocytochemistry/Immunofluorescence: SLC16A10 Antibody [NBP1-80700] - Staining of human cell line CACO-2 shows localization to cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A10 Antibody [NBP1-80700] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SLC16A6 Antibody
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Immunohistochemistry: SLC16A6 Antibody [NBP2-31578] - Staining of human heart muscle shows strong membranous positivity in myocytes.
Species: Hu
Applications: IHC, IHC-P

Publications for SLC16A3 Antibody (NBP1-81251)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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Review for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-81251:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
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reviewed by:
Anonymous
 WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
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FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQs).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Control Lysate(s)

SLC16A3 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Western Blot SLC16A3 NBP1-81251
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 Anonymous
06/04/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot