SLC16A3 Antibody

Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human lung shows strong membranous positivity in macrophages.

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG lysates(s)

Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa lysates(s).

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SLC16A3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC16A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:250
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:33212210), IHC-FR reactivity reported in (PMID:24692237), IB reactivity reported in (PMID: 28978054). Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control Peptide:

  SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32400970). Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

• MCT 3
• MCT3
• MCT3MCT 4
• MCT4
• MCT4MGC138472
• MGC138474
• Monocarboxylate transporter 3
• monocarboxylate transporter 4
• SLC16A3
• solute carrier family 16 (monocarboxylic acid transporters), member 3
• Solute carrier family 16 member 3
• solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Background

Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell. This transport process is carried out by a family of monocarboxylate transporters (MCTs),which function as proton symports and are stereoselective for L-lactate.The MCT family consists of at least eight members, MCT1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm. MCT1 is widely expressed and is the major form of MCTs in tumor cells and erythrocytes. MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC16A3 Antibody (NBP1-81251)(6)

Publications using NBP1-81251

• Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites 2021-08-06 [PMID: 34436459] (WB, Human)
• Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett 2020-11-16 [PMID: 33212210] (IF/IHC, WB, ICC/IF, Human)
  
  Details:
  Immunofluorescence analysis and immunohistochemical analysis of human brain samples. Western blot analysis performed in HeLa and HT-29.
• Lahiguera A, HyroSSovA P, Figueras A et al. Tumors defective in homologous recombination rely on oxidative metabolism: relevance to treatments with PARP inhibitors EMBO Mol Med 2020-05-13 [PMID: 32400970] (IHC-P, Mouse)
• Ilina Elena I, Armento Angela, Sanchez Leticia Garea et al. Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux. Oncotarget 2017-06-27 [PMID: 28978054] (IB, Human)
• Silagi ES, Schoepflin ZR, Seifert EL et al. Bicarbonate Recycling by HIF-1-dependent Carbonic Anhydrase isoforms 9 and 12 is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells J. Bone Miner. Res. 2017-09-23 [PMID: 28940640] (Human)
• Nijland PG, Michailidou I, Witte ME et al. Cellular distribution of glucose and monocarboxylate transporters in human brain white matter and multiple sclerosis lesions. Glia 2014-04-01 [PMID: 24692237] (IHC-Fr, Human)

Review for SLC16A3 Antibody (NBP1-81251) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Gregg Semenza WB Human 06/04/2015

Summary

• Application: Western Blot
• Species: Human

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQs).

1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
   • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Bioinformatics

• Gene Symbol: SLC16A3
• Entrez:
  • 9123(Human)
  • 80878(Rat)
• Uniprot:
  • O15427(Human)