SLC16A3 Antibody

Images

 
Western Blot: SLC16A3 Antibody [NBP1-81251] - analysis of SLC16A3 in human breast cancer cell lines using anti-SLC16A3 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251MG shows positivity in plasma membrane.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: SLC16A3 Antibody [NBP1-81251] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).
Western Blot: SLC16A3 Antibody [NBP1-81251] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western lane view shows a specific band for SLC16A3 in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western lane view shows a specific band for SLC16A3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG lysates(s).
Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa lysates(s).

Product Details

Summary
Reactivity Hu, Rt, MouseSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit

Order Details

SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Simple Western 1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation/permeabilization: PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24692237).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SLC16A3 Protein (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:

Publications
Read Publication using
NBP1-81251 in the following applications:

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC16A3 Antibody (NBP1-81251)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Human
(1)
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Reviews for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-81251:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
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reviewed by:
Anonymous
WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQ).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Secondary Antibodies

 

Isotype Controls

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Blogs on SLC16A3

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot

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