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SLC16A3 Antibody

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Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Specificity
Specificity of human SLC16A3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24692237).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
SLC16A3 Lysate (NBP2-64667)
Control Peptide
SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:
Publications
Read Publications using
NBP1-81251 in the following applications:

Reactivity Notes

Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.
Immunohistochemistry-Paraffin: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - IHC staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: MCT1 Antibody (2F9) [NBP2-03626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCT1.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] Staining of paraffin-embedded Human tonsil using anti-MCT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCT1 mouse monoclonal antibody.
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Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: MCAT Antibody [NBP1-83936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MCAT Antibody [NBP1-83936] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Species: Hu
Applications: WB, IHC, IHC-P
MCPH1 Antibody
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Western Blot: MCT2 Antibody [NBP1-87846] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MCT2 Antibody [NBP1-87846] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Species: Hu
Applications: WB, IHC, IHC-P
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Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(5 Publications)
Species: Hu
Applications: WB, Simple Western, IHC
SLC16A4 Antibody
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Immunocytochemistry/Immunofluorescence: SLC16A4 Antibody [NBP2-13315] - Immunofluorescent staining of human cell line BJ shows localization to microtubules.
Immunohistochemistry-Paraffin: SLC16A4 Antibody [NBP2-13315] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
Species: Hu
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Glut1 Antibody
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Western Blot: Glut1 Antibody [NB110-39113] - Analysis of GLUT1 on A. mouse kidney membrane protein and B. rat kidney membrane protein.
Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
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(6 Reviews)
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CS Citrate
Synthase Antibody (17
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Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [1761].
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.
(1 Review)
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
RPE Antibody
NBP1-56853
Western Blot: RPE Antibody [NBP1-56853] - Human Muscle lysate, concentration 0.2-1 ug/ml.
Immunocytochemistry/Immunofluorescence: RPE Antibody [NBP1-56853] - Antibody 0 Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm but only in connective tissue cells in the interlobular septum, very low tissue distribution Primary Antibody
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Applications: WB, ICC/IF, IHC
MCT8/SLC16A2
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Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining in human liver and skeletal muscle tissues. Corresponding SLC16A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.
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Glut4 Antibody
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Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. This image was submitted via customer review.
Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
(3 Reviews)
(8 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody. Image from verified customer review.
(32 Reviews)
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Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
HLA DQA1 Antibody
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Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Species: Hu
Applications: WB, IHC, IHC-P
CTLA-4 Antibody
[Unconjugated]
AF-386-PB
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA‑4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(2 Reviews)
(2 Publications)
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
SLC16A10 Antibody
NBP1-80700
Immunocytochemistry/Immunofluorescence: SLC16A10 Antibody [NBP1-80700] - Staining of human cell line CACO-2 shows localization to cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A10 Antibody [NBP1-80700] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SLC16A6 Antibody
NBP2-56865
Immunocytochemistry/Immunofluorescence: SLC16A6 Antibody [NBP2-56865] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.
Species: Hu
Applications: ICC/IF

Publications for SLC16A3 Antibody (NBP1-81251)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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Review for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-81251:
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WB
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Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
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reviewed by:
Anonymous
 WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
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ICC/IF Video Protocol

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQs).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Positive Control Lysate(s)

SLC16A3 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Diseases for SLC16A3 Antibody (NBP1-81251)

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Western Blot SLC16A3 NBP1-81251
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 Anonymous
06/04/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot