SLC16A3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human lung shows strong membranous positivity in macrophages.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:250
  • Western Blot
Application Notes
Use in WB reported in scientific literature (PMID:33212210), IHC-FR reactivity reported in (PMID:24692237), IB reactivity reported in (PMID: 28978054). Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:

Publications
Read Publications using
NBP1-81251 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific publication (PMID: 32400970). Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-81251
Species: Hu, Mu
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for SLC16A3 Antibody (NBP1-81251)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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IB
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(2)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using NBP1-81251 Applications Species
Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites Aug 6 2021 [PMID: 34436459] (WB, Human) WB Human
Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett Nov 16 2020 [PMID: 33212210] (ICC/IF, WB, IHC, Human)

Details:
Immunofluorescence analysis and immunohistochemical analysis of human brain samples. Western blot analysis performed in HeLa and HT-29.
ICC/IF, WB, IHC Human
Lahiguera A, HyroSSovA P, Figueras A et al. Tumors defective in homologous recombination rely on oxidative metabolism: relevance to treatments with PARP inhibitors EMBO Mol Med May 13 2020 [PMID: 32400970] (IHC-P, Mouse) IHC-P Mouse
Ilina Elena I, Armento Angela, Sanchez Leticia Garea et al. Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux. Oncotarget 2017 Jun 27 [PMID: 28978054] (IB, Human) IB Human
Silagi ES, Schoepflin ZR, Seifert EL et al. Bicarbonate Recycling by HIF-1-dependent Carbonic Anhydrase isoforms 9 and 12 is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells J. Bone Miner. Res. 2017 Sep 23 [PMID: 28940640] (Human) Human
Nijland PG, Michailidou I, Witte ME et al. Cellular distribution of glucose and monocarboxylate transporters in human brain white matter and multiple sclerosis lesions. Glia 2014 Apr 01 [PMID: 24692237] (IHC-Fr, Human) IHC-Fr Human

Review for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-81251:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
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5
reviewed by:
Verified Customer
WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQs).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Verified Customer
06/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot