Home » SLC16A3 » SLC16A3 Antibodies » SLC16A3 Antibody (NBP1-81251)

SLC16A3 Antibody (NBP1-81251)

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Immunocytochemistry/Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Immunocytochemistry/ Immunofluorescence: SLC16A3 Antibody [NBP1-81251] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Immunohistochemistry-Paraffin: SLC16A3 Antibody [NBP1-81251] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on U-251MG ...read more
Simple Western: SLC16A3 Antibody [NBP1-81251] - Simple Western: SLC16A3 Antibody [NBP1-81251] - Electropherogram image(s) of corresponding Simple Western lane view. SLC16A3 antibody was used at 1:250 dilution on HeLa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SLC16A3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation/permeabilization: PFA/Triton X-100. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24692237).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SLC16A3 Recombinant Protein Antigen (NBP1-81251PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81251 in the following applications:
Publications
Read Publication using
NBP1-81251 in the following applications:

Reactivity Notes

Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC16A3 Antibody

  • MCT 3
  • MCT3
  • MCT3MCT 4
  • MCT4
  • MCT4MGC138472
  • MGC138474
  • Monocarboxylate transporter 3
  • monocarboxylate transporter 4
  • SLC16A3
  • solute carrier family 16 (monocarboxylic acid transporters), member 3
  • Solute carrier family 16 member 3
  • solute carrier family 16, member 3 (monocarboxylic acid transporter 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.
Immunohistochemistry-Paraffin: Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - IHC staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MCTS1 Antibody
(2F9)
NBP2-03626
Western Blot: MCT1 Antibody (2F9) [NBP2-03626] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCT1.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] Staining of paraffin-embedded Human tonsil using anti-MCT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MCT1 Antibody (2F9) [NBP2-03626] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCT1 mouse monoclonal antibody.
Species: Hu
Applications: WB, IHC, IHC-P
Chymase/CMA1/Mast
Cell Chymase Antibody ...
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Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(2 Reviews)
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Applications: WB, IP
MCAT Antibody
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Western Blot: MCAT Antibody [NBP1-83936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MCAT Antibody [NBP1-83936] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Species: Hu
Applications: WB, IHC, IHC-P
MCPH1 Antibody
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
(1 Review)
(3 Publications)
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MCT2 Antibody
NBP1-87846
Western Blot: MCT2 Antibody [NBP1-87846] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MCT2 Antibody [NBP1-87846] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Species: Hu
Applications: WB, IHC, IHC-P
EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.        
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(5 Publications)
Species: Hu
Applications: WB, Simple Western, IHC
SLC16A4 Antibody
NBP2-13315
Immunohistochemistry-Paraffin: SLC16A4 Antibody [NBP2-13315] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: IHC, IHC-P
Glut1 Antibody
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective
Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.
(5 Reviews)
(15 Publications)
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Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CS Citrate
Synthase Antibody (17
NBP2-43648
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [1761] diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.
(1 Review)
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P
RPE Antibody
NBP1-86286
Western Blot: RPE Antibody [NBP1-86286] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404697)
Immunohistochemistry-Paraffin: RPE Antibody [NBP1-86286] - Staining of human skin shows strong cytoplasmic positivity in subsets of epidermal cells.
Species: Hu
Applications: WB, IHC, IHC-P
MCT8/SLC16A2
Antibody
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Immunocytochemistry/Immunofluorescence: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cell line U-251 MG shows localization to plasma membrane.
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected
Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
SLC16A5 RNAi
H00009121-R01
Species: Hu
Glut4 Antibody
NBP1-49533
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
(2 Reviews)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review.
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
(32 Reviews)
(679 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, TCS, LA
HLA DQA1 Antibody
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody [NBP1-84550] - Staining of human cell line U-251MG shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
SLC16A10 Antibody
NBP1-80700
Immunocytochemistry/Immunofluorescence: SLC16A10 Antibody [NBP1-80700] - Staining of human cell line CACO-2 shows localization to cell junctions & vesicles.
Immunohistochemistry-Paraffin: SLC16A10 Antibody [NBP1-80700] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SLC16A6 Antibody
NBP2-56865
Immunocytochemistry/Immunofluorescence: SLC16A6 Antibody [NBP2-56865] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.
Species: Hu
Applications: ICC/IF
SLC16A3 Antibody
NBP1-81251
SLC16A3 Antibody
Species: Hu, Mu
Applications: Simple Western, ICC/IF, IHC, IHC-P

Publications for SLC16A3 Antibody (NBP1-81251)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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Review for SLC16A3 Antibody (NBP1-81251) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-81251:
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Images Ratings Applications Species Date Details
Western Blot SLC16A3 NBP1-81251
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reviewed by:
Anonymous
 WB Human 06/04/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

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ICC/IF Video Protocol

FAQs for SLC16A3 Antibody (NBP1-81251). (Showing 1 - 1 of 1 FAQ).

  1. Does the SLC16A3 Antibody (NBP1-81251) also work on frozen sections?
    • Thank you for contacting Novus Biologicals Technical support. NBP1-81251 has not yet been tested or validated for the use on frozen sections. It is validated and guaranteed to work in IHC on paraffin embedded tissues.

Secondary Antibodies

 

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Western Blot SLC16A3 NBP1-81251
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 Anonymous
06/04/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol SLC16A3
Entrez
Uniprot

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