| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-59656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89574-25ul | |||
F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="Mouse Anti-Human MCT1/SLC16A1 Monoclonal Antibody (Catalog # MAB8275) was detected in (A) HEK293 human embryonic kidney cell line transfected with human MCT1/SLC16A1 and eGFP or (B) Irrelevant transfectants followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58825-25ul | |||
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MCT1/SLC16A1 Antibody NBP1-59656 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59656-img0010.jpg)
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.](http://images.novusbio.com/fullsize/Monocarboxylic-acid-transporter-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59656-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.](http://images.novusbio.com/fullsize/Monocarboxylic-acid-transporter-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59656-img0006.jpg)
![Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.](http://images.novusbio.com/fullsize/Monocarboxylic-acid-transporter-1-Antibody-Western-Blot-NBP1-59656-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Immunofluorescence detection of ion transporters & water transporters in differentiated & CST- SPs. Na+/K+-ATP isoform ATP1A1, water transporter Aquaporin 1, SLC4A11, Bicarbonate transporter NBCe1, Na+-H+ exchanger NHE1 & ZO-1 as an endothelial barrier to complements fluid transport, were all selectively expressed in differentiated SPs, but were almost null in the cell-state-transitioned SPs. Monocarboxylic acid transporter 4 (MCT4) was expressed in the former, but not in the latter SPs, whereas the isomer MCT1 was selectively expressed in the latter SPs, but not in the former SPs. Differentiated SPs were obtained from the same donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 (differentiated SPs, CD44-/ + proportion: 86.7%). CST-SPs were obtained from the separate donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 + 1-µM SB431542 (SB4) + 10-µM SB203580 (SB2) + 5-ng/mL epidermal growth factor (EGF). The differentiated SPs were fixed at 37 days of the second passage, & the CST SPs were fixed at 38 days of the second passage. Bars: 100 µm. Experiments were repeated four times. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59656_rabbit-polyclonal-mct1-slc16a1-antibody-31020241616043.jpg)
![Western Blot: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Blockade of MCT1 inhibits the effects of optogenetic stimulation of sympathetic efferent fibers of BAT. (a) Cropped images of western blotting showing expression of MCT1 in the mitochondrial fractions (n = 2 mice). Full-length blots are presented in Supplementary Figure 2. VDAC: voltage-dependent anion channel, Cyt. C: cytochrome C, Cadhe: Cadherin (b) Pooled data from 6 mice showing changes in Slc16a1 (Mct1) mRNA expression with (filled square) & without (open circle) stimulation of sympathetic innervation of BAT (***p < 0.001, unpaired t-test). Data are shown as mean ± SEM. (c) Pooled data showing effects of blockade of the MCT1 on optogenetically induced increase in body temperature & glucose uptake (n = 5 mice). Data are shown as mean ± SEM. (d) Plot showing relative Slc16a1 (Mct1) mRNA expression in mice injected with control or Slc16a1 shRNA into BAT (n = 6 mice, ***p < 0.001, unpaired t-test). Data are shown as mean ± SEM. (e) Western blotting to show knockdown of the Mct1 gene in BAT injected with Mct1 shRNA. Cropped images of western blotting showing knockdown of MCT1 protein (upper panel). Plot showing relative expression of MCT1 protein (bottom panel, *p < 0.05, unpaired t-test). Data are shown as mean ± SEM. (f) Pooled data showing that mice injected with Slc16a1 (Mct1) shRNA into the BAT pad showed no response to optogenetic stimulation. Data are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25265-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59656_rabbit-polyclonal-mct1-slc16a1-antibody-310202416154641.jpg)
![Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Immunofluorescence detection of ion transporters & water transporters in differentiated & CST- SPs. Na+/K+-ATP isoform ATP1A1, water transporter Aquaporin 1, SLC4A11, Bicarbonate transporter NBCe1, Na+-H+ exchanger NHE1 & ZO-1 as an endothelial barrier to complements fluid transport, were all selectively expressed in differentiated SPs, but were almost null in the cell-state-transitioned SPs. Monocarboxylic acid transporter 4 (MCT4) was expressed in the former, but not in the latter SPs, whereas the isomer MCT1 was selectively expressed in the latter SPs, but not in the former SPs. Differentiated SPs were obtained from the same donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 (differentiated SPs, CD44-/ + proportion: 86.7%). CST-SPs were obtained from the separate donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 + 1-µM SB431542 (SB4) + 10-µM SB203580 (SB2) + 5-ng/mL epidermal growth factor (EGF). The differentiated SPs were fixed at 37 days of the second passage, & the CST SPs were fixed at 38 days of the second passage. Bars: 100 µm. Experiments were repeated four times. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59656_rabbit-polyclonal-mct1-slc16a1-antibody-3102024161605.jpg)
![Simple Western: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - CNOT3 is essential for beta -cell maturation & identity.a qPCR analysis of progenitor cells/dedifferentiation markers, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 4–6). b Immunoblot analysis of ALDH1A3 in islet lysates from 8-week-old control & Cnot3 beta KO mice. This blot is a representative of three different blots. c Band quantification of an immunoblot of ALDH1A3 (n = 3) in Fig. 4b. d qPCR analysis of beta -cell-specific functional mRNAs expression categorized as beta -cell-specific transcription factors, glycolytic pathway, insulin granule maturation, & insulin secretion mRNAs, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 3–7). e Co-immunofluorescence staining of MAFA (green), GLUT2 (green), & insulin (magenta) in pancreatic sections from 8-week-old control & Cnot3 beta KO mice. A scale bar represents 25 µm. Representative results from four 8-week-old mice from each genotype are shown. f qPCR analysis of immature beta -cell markers, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 5–7). g Immunoblot analysis of MCT1 & LDHA in islet lysates from 8-week-old control & Cnot3 beta KO mice. This blot is a representative of three different blots. h Band quantification of immunoblot of MCT1 & LDHA (n = 3). Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32859966), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59656_rabbit-polyclonal-mct1-slc16a1-antibody-31020241616564.jpg)
![Western Blot: MCT1/SLC16A1 Antibody [NBP1-89574] - Analysis in human cell lines HEK293 and MCF-7 using anti-SLC16A1 antibody. Corresponding SLC16A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Western-Blot-NBP1-89574-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: MCT1/SLC16A1 Antibody [NBP1-89574] - Analysis in human cell lines HEK293 and MCF-7 using anti-SLC16A1 antibody. Corresponding SLC16A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-89574] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89574-img0022.jpg)
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-89574] - Staining of human colon shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89574-img0019.jpg)
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-89574] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89574-img0021.jpg)
![Western Blot: MCT1/SLC16A1 Antibody [H00006566-B01P] - Analysis of SLC16A1 expression in transfected 293T cell line by SLC16A1 polyclonal antibody. Lane 1: SLC16A1 transfected lysate(55 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Western-Blot-H00006566-B01P-img0004.jpg "Western Blot: MCT1/SLC16A1 Antibody [H00006566-B01P] - Analysis of SLC16A1 expression in transfected 293T cell line by SLC16A1 polyclonal antibody. Lane 1: SLC16A1 transfected lysate(55 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [H00006566-B01P] - Analysis of purified antibody to SLC16A1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunocytochemistry-Immunofluorescence-H00006566-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody (14612) [NBP2-43838] - Human colon. MCT1 stained by MCT1 antibody [14612] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-14612-Immunohistochemistry-Paraffin-NBP2-43838-img0006.jpg "Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody (14612) [NBP2-43838] - Human colon. MCT1 stained by MCT1 antibody [14612] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT1 antibody [GT14612] (NBP2-43838) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43838_mouse-monoclonal-mct1-slc16a1-antibody-14612-western-blot-2611202518185024.jpg)
![MCT1 antibody [GT14612] detects MCT1 protein at cell membrane by immunofluorescent analysis.<br>Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: MCT1 protein stained by MCT1 antibody [GT14612] (NBP2-43838) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43838_mouse-monoclonal-mct1-slc16a1-antibody-14612-immunocytochemistry-immunofluorescence-2611202518372415.jpg)
![Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: MCT1/SLC16A1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 47 KDa. Observed band size: 54 KDa.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-9D12-Western-Blot-NBP3-15184-img0003.jpg "Western Blot: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: MCT1/SLC16A1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 47 KDa. Observed band size: 54 KDa.")
![Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-9D12-Immunohistochemistry-NBP3-15184-img0002.jpg)
![Immunohistochemistry: MCT1/SLC16A1 Antibody (9D12) [NBP3-15184] - IHC image of MCT1/SLC16A1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-9D12-Immunohistochemistry-NBP3-15184-img0001.jpg)
![Western Blot: MCT1/SLC16A1 Antibody [NBP1-59879] - HT1080 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Western-Blot-NBP1-59879-img0002.jpg "Western Blot: MCT1/SLC16A1 Antibody [NBP1-59879] - HT1080 cell lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59879] - Formalin Fixed Paraffin; Embedded Tissue: Human Bronchial Epithelial Tissue; Observed Staining: Membrane and cytoplasmic; Primary Antibody Concentration: 1:100](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-59879-img0003.jpg)
![Western Blot: Monocarboxylic acid transporter 1 Overexpression Lysate (Adult Normal) [NBP2-11461] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monocarboxylic acid transporter 1.](http://images.novusbio.com/fullsize/Monocarboxylic-acid-transporter-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11461-img0001.jpg "Western Blot: Monocarboxylic acid transporter 1 Overexpression Lysate (Adult Normal) [NBP2-11461] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monocarboxylic acid transporter 1.")
![Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP2-58825] - Staining of human cell line HaCaT shows localization to plasma membrane, cell junctions & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58825-img0001.jpg "Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP2-58825] - Staining of human cell line HaCaT shows localization to plasma membrane, cell junctions & vesicles. Antibody staining is shown in green.")
![Western Blot: Monocarboxylic acid transporter 1 Overexpression Lysate (Adult Normal) [NBL1-16013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monocarboxylic acid transporter 1.](http://images.novusbio.com/fullsize/Monocarboxylic-acid-transporter-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16013-img0002.jpg "Western Blot: Monocarboxylic acid transporter 1 Overexpression Lysate (Adult Normal) [NBL1-16013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monocarboxylic acid transporter 1.")
![Western Blot: MCT1/SLC16A1 Antibody (S06-7F9) [NBP3-15057] - Western blot detection of MCT1/SLC16A1 in K562, Hela cell lysates using MCT1/SLC16A1 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/MCT1-SLC16A1-Antibody-S06-7F9-Western-Blot-NBP3-15057-img0001.jpg "Western Blot: MCT1/SLC16A1 Antibody (S06-7F9) [NBP3-15057] - Western blot detection of MCT1/SLC16A1 in K562, Hela cell lysates using MCT1/SLC16A1 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.")
