Mcl-1 Antibody (SI16-04)

Images

 
Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Mcl-1 antibody. The section was pre-treated using heat mediated ...read more
Flow Cytometry: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of Jurkat cells with MCL1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). ...read more
Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of MCL1 on different lysates using anti-MCL1 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Mouse heart
Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human breast tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human pancreas tissue using anti-MCL1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SI16-04
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Mcl-1 Antibody (SI16-04) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Mcl-1 aa 120-160. (SwissProt: Q07820 Human;SwissProt: P97287 Mouse;SwissProt: Q9Z1P3 Rat)
Localization
Membrane, Cytoplasm, Mitochondrion, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MCL1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Mcl-1 Antibody (SI16-04)

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MCL1