MCAM/CD146 Antibody

Images

 
Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 50 ug rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: MCAM/CD146 Antibody [NBP2-15778] - HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Paraffin-embedded mouse skin. CD146 antibody [C1C3] dilution: 1:500.
Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Paraffin-embedded mouse colon. CD146 antibody [C1C3] dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MCAM/CD146 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-15778.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCAM/CD146 Antibody

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (NBP2-15778)(1)

We have publications tested in 1 confirmed species: Mouse.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCAM
Entrez
Uniprot