MCAM/CD146 Antibody - Azide Free

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MCAM/CD146 Antibody [NBP2-15778] - HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse placenta. Green: CD146 stained by CD146 antibody [C1C3] diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 50 ug rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 30 ug HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:10000

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - J5 xenograft, using CD146 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse colon. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse skin. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

MCAM/CD146 Antibody - Azide Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human MCAM/CD146. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCAM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MCAM/CD146 Antibody - Azide Free

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MCAM/CD146 Antibody (NBP2-15778)(1)

Publication using NBP2-15778

• England CG, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer Mol Pharm 2017-08-22 [PMID: 28825843] (Mouse)

Bioinformatics

• Gene Symbol: MCAM
• Uniprot:
  • P43121(Human)