MCAM/CD146 Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MCAM/CD146 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for MCAM/CD146 at approximately 152 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MCAM/CD146 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
  Met1-Gly559
  Accession # AAA20824
• Specificity: Detects human MCAM/CD46 in Western blots and direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhNCAM-L1, rhTROP-2 and recombinant mouse MCAM is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody [Unconjugated]

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCAM/CD146 Antibody (AF932)(6)

Publications using AF932

• EJ Kim, SG Seo, HS Shin, DJ Lee, JH Kim, DY Lee Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties Clin Orthop Surg, 2017;9(2):239-248. 2017 [PMID: 28567229] (IHC, Human)
• Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, Wang Y, Tang C, Li H, Lu H, Yao X, Jin J, Ma X Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol, 0;118(0):18-30. 0 [PMID: 27520484] (ICC, Human)
• Gu , Zirong, Imai , Fumiyasu, Kim , In Jung, Fujita , Hiroko, Katayama , Kei ichi, Mori , Kensaku, Yoshihara , Yoshihir, Yoshida , Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015;10(3):e0121550. 2015 [PMID: 25826454] (IHC, Mouse)
• Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 2015;10(3):e0121421. 2015 [PMID: 25803686] (IHC-P, Human)
• Keuschnigg , Johannes, Karinen , Sirkku, Auvinen , Kaisa, Irjala , Heikki, Mpindi , John-Pat, Kallioniemi , Olli, Hautaniemi , Sampsa, Jalkanen , Sirpa, Salmi , Marko Plasticity of blood- and lymphatic endothelial cells and marker identification. PLoS ONE, 2013;8(9):e74293. 2013 [PMID: 24058540] (IHC-Fr, Human)
• Rossi E, Sanz-Rodriguez F, Eleno N, Duwell A, Blanco F, Langa C, Botella L, Cabanas C, Lopez-Novoa J, Bernabeu C Endothelial endoglin is involved in inflammation: role in leukocyte adhesion and transmigration. Blood, 2013;121(2):403-15. 2013 [PMID: 23074273] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: MCAM
• Uniprot:
  • AAA20824()