MCAM/CD146 Antibody (P1H12)

Images

 
Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Analysis using the FITC conjugate of NBP1-43346. Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (blue ...read more
Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Rb, Rt(-)Species Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
P1H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

MCAM/CD146 Antibody (P1H12) Summary

Immunogen
The immunogen for this antibody was CD146.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for MCAM/CD146 Antibody (P1H12)

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

The monoclonal antibody P1H12 recognizes human CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCAM
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