MCAM/CD146 Antibody (MUC18/1130)

Images

 
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Melanoma stained with MUC18 Monoclonal Antibody (MUC18/1130)
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (MUC18/1130) [NBP2-44512] - Human Tonsil stained with MUC18 Monoclonal Antibody (MUC18/1130)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
MUC18/1130
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

MCAM/CD146 Antibody (MUC18/1130) Summary

Immunogen
Recombinant human MUC18 protein
Localization
Cell surface
Specificity
The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 11q23.3
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-44512 in the following applications:

Publications
Read Publication using NBP2-44512.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MCAM/CD146 Antibody (MUC18/1130)

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (NBP2-44512)(1)

Reviews for MCAM/CD146 Antibody (NBP2-44512) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, uman tissue.

Reviews using NBP2-44512:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(1)
uman tissue
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MCAM/CD146 NBP2-44512
Enlarge
reviewed by:
Christopher England
IHC-P uman tissue 12/05/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedbrain and pancreatic tissue
Speciesuman tissue
Lot4162-4P150217

Comments

CommentsThe antibody was used at a ratio of 1:200.
Immunohistochemistry-Paraffin MCAM/CD146 NBP2-44512
Enlarge
reviewed by:
Christopher England
IHC-P Human 06/04/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Normal Tissue Microarray
SpeciesHuman
Lot4162-4P150217

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FAQs for MCAM/CD146 Antibody (NBP2-44512) (0)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Christopher England
12/05/2016
Application: IHC-P
Species: uman tissue

Christopher England
06/04/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MCAM
Entrez
Uniprot