MBD2 Antibody

Images

 
Western Blot: MBD2 Antibody [NB100-55415] - (0.3ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MBD2 Antibody Summary

Immunogen
Peptide with sequence C-RNDPLNQNKGKPDLN corresponding to internal region according to NP_003918.1.
Epitope
RNDPLNQNKGKPDLN
Specificity
This antibody is expected to resognize isoform 1 (NP_003918.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MBD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 48-50 kDa band observed in Jurkat lysates is reported in scientific literature (PMID: 16980624). IHC-P: Human colon shows nuclear stianing of enterocytes.
Control
Jurkat Whole Cell Lysate
Publications
Read Publications using NB100-55415.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MBD2 Antibody

  • demethylase
  • DKFZp586O0821
  • DMTase
  • methyl-CpG binding domain protein 2
  • methyl-CpG-binding domain protein 2
  • Methyl-CpG-binding protein MBD2
  • NY-CO-41

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. This protein may function as a mediator of the biological consequences of the methylation signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBD2 Antibody (NB100-55415)(2)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MBD2
Entrez
Uniprot