MAP2 Antibody (5H11)

Images

 
Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of different tissue lysates using mouse mAb to MAP2A/B, NBP1-92711, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (5H11) [NBP1-92711] - Single cultured astrocytes stained with MAP2 (red) and Myelin (green). Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
5H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

MAP2 Antibody (5H11) Summary

Immunogen
Full length purified bovine MAP2 protein. Epitope maps to 1057-1507 of the human sequence.
Localization
Cytoplasm > cytoskeleton
Marker
Neuronal Dendritic Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This MAP2 (5H11) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 280 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. ICC/IF reported in a verified customer review.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-92711 in the following applications:

Publications
Read Publications using
NBP1-92711 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MAP2 Antibody (5H11)

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • microtubule-associated protein 2

Background

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons. The HGNC name for this protein is MAP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-92711
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for MAP2 Antibody (NBP1-92711)(3)

Review for MAP2 Antibody (NBP1-92711) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-92711:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence MAP2 NBP1-92711
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reviewed by:
Anonymous
IF Mouse 04/29/2014
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Summary

ApplicationImmunofluorescence
Sample TestedRat astrocytes
SpeciesMouse
Lot92412

Blocking

Blocking Details10% normal goat serum(NGS), 1hr at Room temperature(RT)

Primary Anitbody

Dilution Ratiodiluted 1:500 in 1x PBS with 0.1%NGS, 2Hrs at RT

Secondary Antibody

Secondary Descriptiongoat anti-mouse
Secondary Manufacturer Cat#Vector BA-9200
Secondary Concentration1:500

Details

Wash Description3washes in 1x PBS 5mins each

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NBP1-92711) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-92711V
FITC Labeled NBP1-92711F

Additional MAP2 Products

Bioinformatics Tool for MAP2 Antibody (NBP1-92711)

Discover related pathways, diseases and genes to MAP2 Antibody (NBP1-92711). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MAP2 Antibody (NBP1-92711)

Discover more about diseases related to MAP2 Antibody (NBP1-92711).
 

Pathways for MAP2 Antibody (NBP1-92711)

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PTMs for MAP2 Antibody (NBP1-92711)

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Research Areas for MAP2 Antibody (NBP1-92711)

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Blogs on MAP2.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amyloid beta (a polypepti...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

5
5
1
4
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3
0
2
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1
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Anonymous
04/29/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol MAP2
Entrez
Uniprot