MAP2 Antibody (5H11) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clone
5H11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

MAP2 Antibody (5H11) [DyLight 405] Summary

Immunogen
Bovine brain high molecular weight MAP2 preparation.
Localization
Cytoplasm > cytoskeleton
Marker
Neuronal Dendritic Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAP2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This MAP2 (5H11) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 280 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MAP2 Antibody (5H11) [DyLight 405]

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • microtubule-associated protein 2

Background

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons. The HGNC name for this protein is MAP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NBP1-92711V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC Labeled NBP1-92711F

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Bioinformatics Tool for MAP2 Antibody (NBP1-92711V)

Discover related pathways, diseases and genes to MAP2 Antibody (NBP1-92711V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MAP2 Antibody (NBP1-92711V)

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Research Areas for MAP2 Antibody (NBP1-92711V)

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Blogs on MAP2.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Bioinformatics

Gene Symbol MAP2
Entrez