Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:1000-1:3000
This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS pH 7.2
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-42827 in the following applications:
Human, mouse, rat, chicken, Xenopus, fish and reptile.
Alternate Names for MAP1B [p Thr1265] Antibody - SuperBUGS
microtubule-associated protein 1B
MAP1B (microtubule-associated protein 1B) is the major cytoskeletal constituent of developing nervous system wherein it plays a key role in neuronal migration as well as differentiation. MAP1B interacts with LC1, ANP32A, TIAM2, GAN, DAPK1, tubulin tyrosine TTL etc. and binds at least two tubulin subunits in the polymer which is critical in nucleating microtubule polymerization as well as in stabilizing the microtubules. MAP1B is one of the major phosphorylation substrates for GSK-3beta which phosphorylates it at Ser1260/Thr1265 in differentiating neurons, a molecular switch for MAP1B mediated regulation of microtubule dynamics in the growing axons and the growth cones. MAP1B acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation/membrane blebbing and also brings about tyrosination of alpha-tubulin in neuronal microtubules along with its essential role in synaptic maturation. MAP1B is downstream of various signalling pathways that stimulate neurite growth or direct growth cone pathfinding, e.g. laminin, netrins, neurotrophins, wingless/integration site, or that influence neuronal migration, e.g. reelin. MAP1B is implicated in a number of neurological disorders, such as fragile X syndrome and giant axonal neuropathy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MAP1B Antibody (NBP1-42827). (Showing 1 - 1 of 1 FAQs).
A customer is interested in a MAP1B antibody and found NBP1-42827 MAP1B [p Thr1265] Antibody (SuperBUGS). She would like to know what this SuperBUGS means and how it is different from a normal MAP1B antibody?
In regards to the MAP1B SuperBUGS antibody (Novus Product NBP1-42827), SuperBUGS is simply the clone name for this MAP1B antibody that is specific for MAPB1 that is phosphorylated Thr1265. Other MAPB1 antibodies may not be specific for this phosphorylation.