MAP1B [p Thr1265] Antibody - SuperBUGS

Images

 
Western Blot: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fi, Re, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.12 mg/ml

MAP1B [p Thr1265] Antibody - SuperBUGS Summary

Immunogen
Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]
Modification
p Thr1265
Marker
Neuronal Marker
Specificity
Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
Application Notes
This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS pH 7.2

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
320 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-42827 in the following applications:

Reactivity Notes

Human, mouse, rat, chicken, Xenopus, fish and reptile.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.12 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAP1B [p Thr1265] Antibody - SuperBUGS

  • DKFZp686E1099
  • DKFZp686F1345
  • FLJ38954
  • FUTSCH
  • MAP-1B
  • MAP5
  • microtubule-associated protein 1B

Background

MAP1B (microtubule-associated protein 1B) is the major cytoskeletal constituent of developing nervous system wherein it plays a key role in neuronal migration as well as differentiation. MAP1B interacts with LC1, ANP32A, TIAM2, GAN, DAPK1, tubulin tyrosine TTL etc. and binds at least two tubulin subunits in the polymer which is critical in nucleating microtubule polymerization as well as in stabilizing the microtubules. MAP1B is one of the major phosphorylation substrates for GSK-3beta which phosphorylates it at Ser1260/Thr1265 in differentiating neurons, a molecular switch for MAP1B mediated regulation of microtubule dynamics in the growing axons and the growth cones. MAP1B acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation/membrane blebbing and also brings about tyrosination of alpha-tubulin in neuronal microtubules along with its essential role in synaptic maturation. MAP1B is downstream of various signalling pathways that stimulate neurite growth or direct growth cone pathfinding, e.g. laminin, netrins, neurotrophins, wingless/integration site, or that influence neuronal migration, e.g. reelin. MAP1B is implicated in a number of neurological disorders, such as fragile X syndrome and giant axonal neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP1B Antibody (NBP1-42827)(8)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Fish, Reptile, Xenopus.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(5)
IHC-Fr
(4)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(4)
Chicken
(2)
Fish
(1)
Reptile
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-42827 Applications Species
Ketschek A, Jones S, Spillane M et al. Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching Dev Neurobiol 2015 Apr 02 [PMID: 25846486] (ICC/IF, Chicken) ICC/IF Chicken
Ma QL, Zuo X, Yang F et al. Loss of MAP Function Leads to Hippocampal Synapse Loss and Deficits in the Morris Water Maze with Aging. J. Neurosci. 2014 May 21 [PMID: 24849348] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Capoccia BJ, Jin RU, Kong YY et al. The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation J Clin Invest 2013 Apr 1 [PMID: 23478405] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Marchbank K, Waters S, Roberts RG et al. MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network Int J Cell Biol 2012 [PMID: 22654911] (ICC/IF, Rat) ICC/IF Rat
Tymanskyj SR, Lin S, Gordon-Weeks PR. Evolution of the spatial distribution of MAP1B phosphorylation sites in vertebrate neurons. J Anat. 2010 Apr 9. [PMID: 20408908] (WB, IHC-Fr, ICC/IF, Rat, Reptile, Chicken, Xenopus, Fish) WB, IHC-Fr, ICC/IF Rat, Reptile, Chicken, Xenopus, Fish
Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR. Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. J Cell Sci;122(Pt 14):2424-35. 2009 Jul 15. [PMID: 19549690] (WB, IHC-Fr, Rat) WB, IHC-Fr Rat
Trivedi N, Marsh P, Goold RG, Wood-Kaczmar A, Gordon-Weeks PR. Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons. J Cell Sci;118(Pt 5):993-1005. 2005 Mar 1. [PMID: 15731007] (WB, IHC-Fr, ICC/IF, Rat) WB, IHC-Fr, ICC/IF Rat
Pimentel HI da CA. Glycogen synthase kinase 3[beta] modulation in axonal regeneration. 2011 Universidade de Aveiro. 2011

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP1B Antibody (NBP1-42827). (Showing 1 - 1 of 1 FAQs).

  1. A customer is interested in a MAP1B antibody and found NBP1-42827 MAP1B [p Thr1265] Antibody (SuperBUGS). She would like to know what this SuperBUGS means and how it is different from a normal MAP1B antibody?
    • In regards to the MAP1B SuperBUGS antibody (Novus Product NBP1-42827), SuperBUGS is simply the clone name for this MAP1B antibody that is specific for MAPB1 that is phosphorylated Thr1265. Other MAPB1 antibodies may not be specific for this phosphorylation.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-42827AF405
Alexa Fluor 488 Labeled NBP1-42827AF488
Alexa Fluor 647 Labeled NBP1-42827AF647
Alexa Fluor 700 Labeled NBP1-42827AF700
Biotin Labeled NBP1-42827B
DyLight 405 Labeled NBP1-42827V
DyLight 488 Labeled NBP1-42827G
DyLight 550 Labeled NBP1-42827R
DyLight 650 Labeled NBP1-42827C
FITC Labeled NBP1-42827F
HRP Labeled NBP1-42827H

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MAP1B NBP1-42827

Bioinformatics Tool for MAP1B Antibody (NBP1-42827)

Discover related pathways, diseases and genes to MAP1B Antibody (NBP1-42827). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for MAP1B Antibody (NBP1-42827)

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Blogs on MAP1B.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr...  Read full blog post.

SuperBUGS in the Brain: MAP1B Antibodies & Neuronal Disease Research
Recently, we at Novus Biologicals added a new phospho-MAP1B (phosphorylated microtubule-associated protein 1B) antibody to our antibody database. MAP1B is a developmentally regulated phosphoprotein thought to be involved in the assembly of microtubule...  Read full blog post.

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Bioinformatics

Gene Symbol MAP1B