MAP2 Antibody (4H5)

Images

 
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.
Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IHC-FrFl
Clone
4H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

MAP2 Antibody (4H5) Summary

Immunogen
Full length purified bovine protein, epitope mapped to projection domain of human sequence, between amino acids 631 and 1056.
Localization
Cytoplasm > cytoskeleton
Marker
Neuronal Dendritic Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunohistochemistry Free-Floating
Application Notes
This MAP2 (4H5) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~280 kDa. Use in Immunohistochemistry-free floating and Immunohistochemistry reported in scientific literature (PMID 26909021). IHC-P reported in a verified customer review.
Theoretical MW
280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 5
using
NBP2-25156 in the following applications:

Publications
Read Publication using
NBP2-25156 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAP2 Antibody (4H5)

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • microtubule-associated protein 2

Background

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-25156
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IHC-FrFl

Publications for MAP2 Antibody (NBP2-25156)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, IHC-FrFl.


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IHC
(1)
IHC-FrFl
(1)
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Mouse
(1)
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Reviews for MAP2 Antibody (NBP2-25156) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using NBP2-25156:
Filter by Applications
IF
(2)
IHC-P
(1)
ICC
(1)
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Filter by Species
Mouse
(4)
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Images Ratings Applications Species Date Details
Immunofluorescence MAP2 NBP2-25156
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reviewed by:
Anonymous
IF Mouse 01/12/2016
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
LotNBP2-25156SS
FileView PDF
Immunofluorescence MAP2 NBP2-25156
Enlarge
reviewed by:
Anonymous
IF Mouse 11/08/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedmouse brain
SpeciesMouse
LotNBP2-25156

Blocking

Blocking DetailsNGS

Primary Anitbody

Dilution Ratio1:300 and 1:500 works

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG from Vector
Secondary Concentration1:250

Details

Detection NotesN
Fixation DetailsFixed brain with 1%PFA, permeabilized with acetone and washed with TBS at RT
Wash Description1% TBS 5X/5min

Comments

CommentsThis antibody works very well in mouse tissue.
Immunohistochemistry-Paraffin MAP2 NBP2-25156
Enlarge
reviewed by:
Musaad Alshammari
IHC-P Mouse 10/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse brain
SpeciesMouse
LotNBP2-25156SS
 
reviewed by:
Anonymous
ICC Mouse 10/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse brain
SpeciesMouse

Blocking

Blocking Details5% NGS + 3%DS

Primary Anitbody

Dilution Ratio1:300

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG1
Secondary Manufacturer Cat#Vector
Secondary Concentration1:250

Details

Detection NotesIHC
Fixation DetailsFixation
Wash Description1% TBS

Comments

CommentsThis antibody works very well in a mouse brain tissue.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAP2 Antibody (NBP2-25156) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MAP2 Antibody (NBP2-25156)

Discover related pathways, diseases and genes to MAP2 Antibody (NBP2-25156). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for MAP2 Antibody (NBP2-25156)

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Blogs on MAP2.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amyloid beta (a polypepti...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Anonymous
01/12/2016
Application: IF
Species: Mouse

Anonymous
11/08/2015
Application: IF
Species: Mouse

Musaad Alshammari
10/08/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MAP2
Entrez
OMIM
Uniprot