Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.
Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green:  protein standard (red),  rat brain,  mouse brain, and  ...read more
Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to ...read more
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.
Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.
This MAP2 (4H5) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~280 kDa. Use in Immunohistochemistry-free floating and Immunohistochemistry reported in scientific literature (PMID 26909021). IHC-P reported in a verified customer review.
280 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 4 Reviews rated 5 using NBP2-25156 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% PBS, 50% Glycerol
5mM Sodium Azide
Immunogen affinity purified
Alternate Names for MAP2 Antibody (4H5)
microtubule-associated protein 2
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast tau is found predominantly in neuronal axons.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post.
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