MAP2 Antibody (4H5) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P, IHC-FrFl
Clone
4H5
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

MAP2 Antibody (4H5) [HRP] Summary

Immunogen
MAP2 Antibody (4H5) was developed against full length purified bovine protein, epitope mapped to projection domain of human sequence, between amino acids 631 and 1056.
Localization
Cytoskeleton
Marker
Neuronal Dendritic Marker
Specificity
MAP2 Antibody (4H5) will be reactive to isoforms 1(MAP2B) and isoform 3 (MAP2A).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry Free-Floating
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MAP2 Antibody (4H5) [HRP]

  • DKFZp686I2148
  • MAP2
  • MAP-2
  • MAP2A
  • MAP2B
  • MAP2C
  • Microtubule Associated Protein 2
  • microtubule-associated protein 2
  • MTAP2

Background

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MAP2 Antibody (NBP2-25156H) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25156AF350
Alexa Fluor 405 NBP2-25156AF405
Alexa Fluor 488 NBP2-25156AF488
Alexa Fluor 532 NBP2-25156AF532
Alexa Fluor 594 NBP2-25156AF594
Alexa Fluor 647 NBP2-25156AF647
Alexa Fluor 700 NBP2-25156AF700
Alexa Fluor 750 NBP2-25156AF750
Biotin NBP2-25156B
DyLight 350 NBP2-25156UV
DyLight 405 NBP2-25156V
DyLight 488 NBP2-25156G
DyLight 550 NBP2-25156R
DyLight 594 NBP2-25156DL594
DyLight 650 NBP2-25156C
DyLight 680 NBP2-25156FR
DyLight 755 NBP2-25156IR
FITC NBP2-25156F
HRP NBP2-25156H
Janelia Fluor 549 NBP2-25156JF549
Janelia Fluor 646 NBP2-25156JF646

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Bioinformatics

Gene Symbol MAP2