Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody ) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human esophageal carcinoma.LOXL2 stained by LOXL2 antibody diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Immunohistochemistry-Paraffin-NBP1-32954-img0043.jpg

ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Lysyl Oxidase Homolog 2/LOXL2 antibody detects Lysyl Oxidase Homolog 2/LOXL2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Lysyl Oxidase Homolog 2/LOXL2 stained by Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell lysates from SUM159 cells exposed to either 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. LOXL2 Antibody (NBP1-32954) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Human LOXL2 protein, His tag (0.05 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Fe, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free (NBP1-32954) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Lysyl Oxidase Homolog 2/LOXL2 Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Lysyl Oxidase Homolog 2/LOXL2. The exact sequence is proprietary.
• Localization: Secreted, extracellular space
• Predicted Species: Rat (91%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOXL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:10000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (83%). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Lysyl Oxidase Homolog 2/LOXL2 Antibody - BSA Free

• EC 1.4.3
• EC 1.4.3.-
• LOL2
• LOR2
• LOXL2
• Lysyl Oxidase Homolog 2
• lysyl oxidase related 2
• lysyl oxidase-like 2
• Lysyl oxidase-like protein 2
• Lysyl oxidase-related protein 2
• Lysyl oxidase-related protein WS9-14
• WS9-14

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954)(14)

Publications using NBP1-32954

• Gebril M, Mulder S, Das R et al. Extracellular matrix reorganization during endometrial decidualization Histochemistry and Cell Biology 2025-08-28 [PMID: 40875056]
• Cetin M, Saatci O, Rezaeian AH et Al. A highly potent bi-thiazole inhibitor of LOX rewires collagen architecture and enhances chemoresponse in triple-negative breast cancer Cell Chem Biol 2024-07-09 [PMID: 39043186]
• Yun H, Im HJ, Choe C, Roh S Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells Gene 2022-04-25 [PMID: 35483499] (WB, Human)
• Jiang C, Wang M, Yao W et al. Comprehensive Analysis on Prognosis and Immune Infiltration of Lysyl Oxidase Family Members in Pancreatic Adenocarcinoma With Experimental Verification Frontiers in Molecular Biosciences 2022-04-01 [PMID: 35433829] (Western Blot, Human)
• Novotny T, Eckhardt A, Doubkova M et al. The possible role of hypoxia in the affected tissue of relapsed clubfoot Scientific reports 2022-03-15 [PMID: 35292718] (IF/IHC, Human)
• Yamamoto A, Saito T, Hosoya T et al. Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis Arthritis & rheumatology (Hoboken, N.J.) 2021-12-09 [PMID: 34882985]
• Bharti A, Urs AB, Kumar P. Significance of HIF-1? Expression and LOXL-2 Localization in Progression of Oral Squamous Cell Carcinoma Asian Pacific Journal of Cancer Prevention 2021-02-01 [PMID: 33639646] (Western Blot, Human)
• Cebria-Costa JP, Pascual-Reguant L, Gonzalez-Perez A et al. LOXL2-mediated H3K4 oxidation reduces chromatin accessibility in triple-negative breast cancer cells Oncogene 2019-08-28 [PMID: 31462706] (WB, Human)
• Jeong YJ, Park SH, Mun SH et al. Association between lysyl oxidase and fibrotic focus in relation with inflammation in breast cancer Oncology Letters [PMID: 29434955] (IHC-P, Human)
• Tjin G, White ES, Faiz A et al. Lysyl oxidases regulate fibrillar collagen remodelling in idiopathic pulmonary fibrosis. Dis Model Mech 2017-11-01 [PMID: 29125826] (IHC-P, Human)
• Sato F, Seino-Sudo R, Okada M et al. Lysyl Oxidase Enhances the Deposition of Tropoelastin through the Catalysis of Tropoelastin Molecules on the Cell Surface. Biol. Pharm. Bull. 2017-10-02 [PMID: 28966236] (WB)
• Wong CC, Zhang H, Gilkes DM et al. Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis. J Mol Med (Berl) 2012-07-01 [PMID: 22231744] (WB, Human)
• Querol J Maintaining oxidized H3 in heterochromatin is required for the oncogenic capacity of triple-negative breast cancer cells bioRxiv 2020-01-01 (KD, WB, Human)
• Torres S, Garcia-Palmero I, et al. LOXL2 Is Highly Expressed in Cancer-Associated Fibroblasts and Associates to Poor Colon Cancer Survival. Clin Cancer Res 2015-11-01 [PMID: 26206869] (IF/IHC, Human)

Reviews for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954) (3)

Average Rating: 3.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, cat.

reviewed by: Yongkang Yang WB Human 07/17/2025

Summary

• Application: Western Blot
• Sample Tested: SUM-159PT human breast cancer cell line
• Species: Human
• Lot: 43614

reviewed by: Junhua Yang Simple Western Human 01/25/2023

Summary

• Application: Simple Western
• Sample Tested: Huh7 whole cell lysate
• Species: Human
• Lot: 43516

Comments

• Comments: Cell line: Huh7 (HCC)
  Cell lysate buffer:50mM Tris/150mM Nacl/0.5% NP40/PI
  LOXL2 antibody dilution tried 1:100 and 1:200, the 1:200 is right dilution
  Sample dilution:0.2 mg/ml and 1mg/ml. 1mg/ml is better

reviewed by: Verified Customer ICC cat 01/14/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: cat
• Lot: 42389

Comments

• Comments: Feline cardiac fibroblasts were blocked in 100µl of 10% Goat serum for 30 min and 0.85µl of LOXL2 antibody was added in each well in the 24-well plate. The cells were incubated with the primary antibody at 4C overnight, then were washed 3 times with PBST (5 min each). Goat anti rabbit IgG conjugated with Cy2 were used as secondary. DAPI was also added in for staining of the nuclei.

FAQs for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954). (Showing 1 - 1 of 1 FAQs).

1. What is the appropriate positive control for NBP1-32954 in immunohistochemistry on paraffin embedded sections?
   • According to The Human Protein Atlas Lysyl oxidase homolog 2 protein is expressed in most tissues. You can use any of the tissues with good (at least medium) protein expression levels as your positive control for IHC. Please see the graph and examples of IHC tissue stainings here: https://www.proteinatlas.org/ENSG00000134013-LOXL2/tissue. For example, I see the references for this product successfully stained human lung tissue, ovarian and breast carcinomas. If you wish to purchase a tissue slide to be used as a control, we have some in our catalog. You can find them here and here

Bioinformatics

• Gene Symbol: LOXL2
• Entrez:
  • 4017(Human)
  • 94352(Mouse)
• Uniprot:
  • Q9Y4K0(Human)