Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody Summary
Immunogen |
A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164] |
Localization |
Nucleus. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
KDM4A |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Western Blot 1:25000
- Simple Western 1:500
- Immunocytochemistry/Immunofluorescence 1:100
- Immunohistochemistry 1:50
- Immunohistochemistry-Paraffin 1:50
|
Application Notes |
This JMJD2A antibody is useful for Immunohistochemistry on paraffin tissues, Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~119 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
Control |
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Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS and 30% Glycerol |
Preservative |
0.05% Sodium Azide |
Concentration |
0.53 mg/ml |
Purity |
Immunogen affinity purified |
Notes
Manufactured by Genomic Antibody Technology™. GAT
FAQs
Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody
Background
Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602) (0)
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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