Home » Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A » Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibodies » Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

 
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Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi ...read more
Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Staining of JMJD2A in paraffin embedded mouse pancreas.
Simple Western: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Simple Western lane view shows a specific band for JMJD2A in 0.5 mg/ml of HeLa lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.53 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-49602AF350
Alexa Fluor 405
NBP1-49602AF405
Alexa Fluor 488
NBP1-49602AF488
Alexa Fluor 532
NBP1-49602AF532
Alexa Fluor 594
NBP1-49602AF594
Alexa Fluor 647
NBP1-49602AF647
Alexa Fluor 700
NBP1-49602AF700
Alexa Fluor 750
NBP1-49602AF750
Biotin
NBP1-49602B
DyLight 350
NBP1-49602UV
DyLight 405
NBP1-49602V
DyLight 488
NBP1-49602G
DyLight 550
NBP1-49602R
DyLight 594
NBP1-49602DL594
DyLight 650
NBP1-49602C
DyLight 680
NBP1-49602FR
DyLight 755
NBP1-49602IR
FITC
NBP1-49602F
HRP
NBP1-49602H
Janelia Fluor 549
NBP1-49602JF549
Janelia Fluor 646
NBP1-49602JF646
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Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Simple Western 1:500
  • Western Blot 1:25000
Application Notes
This JMJD2A antibody is useful for Immunohistochemistry on paraffin tissues, Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~119 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Nuclear Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.53 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM3A
  • JHDM3AJMJD2
  • JmjC domain-containing histone demethylation protein 3A
  • JMJD2
  • JMJD2A
  • JMJD2Alysine-specific demethylase 4A
  • jumonji domain containing 2
  • jumonji domain containing 2A
  • Jumonji domain-containing protein 2A
  • KDM4A
  • KIAA0677jumonji C domain-containing histone demethylase 3A
  • Lysine (K)specific Demethylase 4A
  • Lysine (K)-specific Demethylase 4A

Background

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody NBP1-49600 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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(9 Publications)
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JMJD2B Antibody
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Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 (lot NB100-74605-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot NB100-74605-1) of this antibody For blotting immunoprecipitated JMJD2B, NB100-74605 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Knockdown Validated: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level and represses PPARgamma2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. The levels of JMJD2B, H3K9me, H3K9me2, and H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY licence.
Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 uM concentrations for 24 h, and intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA and protein levels were examined by qPCR and western blotting, respectively. Data represent means +/- SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY licence.
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(5 Publications)
Species: Hu, Mu
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Histone H3
[Trimethyl Lys9] Anti ...
NBP1-30141
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
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(21 Publications)
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
MBD2 Antibody
NB100-81657
Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.
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LSD1 Antibody
(1B2E5)
NB100-1762
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY licence.
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JMJD2D Antibody
NBP1-03357
Western Blot: JMJD2D Antibody [NBP1-03357] - Western Blot Image of anti-JMJD2D. Whole cell protein from PC3 (lane 1), SHSY5Y (lane 2), and HeLa (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-JMJD2D in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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(2 Publications)
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Applications: ChIP, ICC/IF (-), WB
53BP1 Antibody
NB100-304
Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.
Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.
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Species: Bt, Bv, Ca, Fi, Gt, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO, WB
Jarid1A Antibody
NB110-40499
Western Blot: Jarid1A Antibody [NB110-40499] - Whole cell lysate (50 ug) from TCMK-1 and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Jarid1A Antibody [NB110-40499] - Detection of human JARID1A/RBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1A/RBP2 antibody NB110-40499 (lot NB110-40499-3) used for IP at 6 ug per reaction. JARID1A/RBP2 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40499-2). For blotting immunoprecipitated JARID1A/RBP2, NB110-40499 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Jarid1A Antibody [NB110-40499] - Detection of Human and Mouse Jarid1A/RBP2 on HeLa whole cell lysate using NB110-40499. Jarid1A/RBP2 was also immunoprecipitated by other rabbit anti-Jarid1A/RBP2 antibodies.
(5 Publications)
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Applications: IP, WB
HP1 alpha Antibody
NB100-2518
Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with rIgG (AF488) isotype control (blue).
Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.
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Applications: Flow-IC, ICC/IF, WB
G9a/EHMT2 Antibody
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PP-A8620A-00
G9a/EHMT2 Antibody (A8620A) [Unconjugated]
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alpha-Defensin 1
Antibody
NBP3-05562
Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP3-05562] - Immunohistochemistry of paraffin-embedded rat heart using alpha-Defensin 1 antibody (NBP3-05562) at dilution of 1:100 (40x lens).
Immunocytochemistry/Immunofluorescence: alpha-Defensin 1 Antibody [NBP3-05562] - Immunofluorescence analysis of HeLa cells using alpha-Defensin 1 antibody (NBP3-05562). Blue: DAPI for nuclear staining.
Western Blot: alpha-Defensin 1 Antibody [NBP3-05562] - Analysis of extracts of various cell lines, using DEFA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
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Lysine
(K)-specific Demethyl ...
NB100-77282
Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Total cell extracts of wild-type (RPE1WT) and KDM3A gene-edited RPE1 mutants (KDM3ACr22, named herein KDM3Anull) immunoblotted with KDM3A, revealing KDM3A-null clones (1, 2, and 8). Image collected and cropped by CiteAb from the following publication (https://rupress.org/jcb/article/216/4/999/52171/KDM3A-coordinates-actin-dynamics-with) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Human cancer cells stained with NB100-77282 and Alexa Fluor 488 conjugated goat-anti-rabbit secondary antibody. DNA stained with Hoechst 33342. Staining shows KDM3A protein in mixed nuclear and cytoplasmic location. ICC/IF image submitted by a verified customer review.
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Detection of Human JMJD1A/JHDM2A on HeLa whole cell lysate using NB100-77282. Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody NB100-77281.
(1 Review)
(9 Publications)
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Applications: ChIP, ICC/IF, IHC, IP, KO, WB
ASCL2/Mash2
Antibody
AF6539
Western blot shows lysates of JAR human choriocarcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of human intestine using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to the base of intestinal crypts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
Species: Hu
Applications: IHC, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2)
NBP2-52486
ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NCOR1 Antibody
NB100-58824
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
(3 Publications)
Species: Hu, Mu
Applications: CHIP-SEQ, ChIP, IP, WB
HDAC1 Antibody
NB100-56340
Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
(2 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB

Publications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602) (0)

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Product General Protocols

View specific protocols for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602) (0)

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Control Lysate(s)

HeLa Nuclear Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-49602AF350
Alexa Fluor 405 NBP1-49602AF405
Alexa Fluor 488 NBP1-49602AF488
Alexa Fluor 532 NBP1-49602AF532
Alexa Fluor 594 NBP1-49602AF594
Alexa Fluor 647 NBP1-49602AF647
Alexa Fluor 700 NBP1-49602AF700
Alexa Fluor 750 NBP1-49602AF750
Biotin NBP1-49602B
DyLight 350 NBP1-49602UV
DyLight 405 NBP1-49602V
DyLight 488 NBP1-49602G
DyLight 550 NBP1-49602R
DyLight 594 NBP1-49602DL594
DyLight 650 NBP1-49602C
DyLight 680 NBP1-49602FR
DyLight 755 NBP1-49602IR
FITC NBP1-49602F
HRP NBP1-49602H
Janelia Fluor 549 NBP1-49602JF549
Janelia Fluor 646 NBP1-49602JF646
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Bioinformatics Tool for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602)

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Bioinformatics

Gene Symbol KDM4A
Entrez
Uniprot