Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Staining of JMJD2A in paraffin embedded mouse pancreas.

Simple Western: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Simple Western lane view shows a specific band for JMJD2A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.53 mg/ml

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free (NBP1-49602) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164]
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Simple Western 1:500
  • Western Blot 1:1000 - 1:2000
• Application Notes: This JMJD2A antibody is useful for Immunohistochemistry on paraffin tissues, Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~119 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 146 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.53 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• JHDM3A
• JHDM3AJMJD2
• JmjC domain-containing histone demethylation protein 3A
• JMJD2
• JMJD2A
• JMJD2Alysine-specific demethylase 4A
• jumonji domain containing 2
• jumonji domain containing 2A
• Jumonji domain-containing protein 2A
• KDM4A
• KIAA0677jumonji C domain-containing histone demethylase 3A
• Lysine (K)specific Demethylase 4A
• Lysine (K)-specific Demethylase 4A

Background

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602)(1)

Publication using NBP1-49602

• Couteau F, Gagné L, Boulay K et al. R-2-hydroxyglutarate-mediated inhibition of KDM4A compromises telomere integrity. Nucleic acids research 2025-06-11 [PMID: 40498073]

Bioinformatics

• Gene Symbol: KDM4A
• Entrez:
  • 9682(Human)
  • 230674(Mouse)
• Uniprot:
  • O75164(Human)