Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody


Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi more
Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Staining of JMJD2A in paraffin embedded mouse pancreas.
Simple Western: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Simple Western lane view shows a specific band for JMJD2A in 0.5 mg/ml of HeLa lysate. This experiment was performed under more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
0.53 mg/ml

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody Summary

A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:25000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This JMJD2A antibody is useful for Immunohistochemistry on paraffin tissues, Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~119 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
HeLa Nuclear Cell Lysate
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Knockout HeLa Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
0.53 mg/ml
Immunogen affinity purified


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM3A
  • JmjC domain-containing histone demethylation protein 3A
  • JMJD2
  • JMJD2A
  • JMJD2Alysine-specific demethylase 4A
  • jumonji domain containing 2
  • jumonji domain containing 2A
  • Jumonji domain-containing protein 2A
  • KDM4A
  • KIAA0677jumonji C domain-containing histone demethylase 3A
  • Lysine (K)specific Demethylase 4A
  • Lysine (K)-specific Demethylase 4A


Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP1-49602) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-49602AF350
Alexa Fluor 405 NBP1-49602AF405
Alexa Fluor 488 NBP1-49602AF488
Alexa Fluor 532 NBP1-49602AF532
Alexa Fluor 594 NBP1-49602AF594
Alexa Fluor 647 NBP1-49602AF647
Alexa Fluor 700 NBP1-49602AF700
Alexa Fluor 750 NBP1-49602AF750
Biotin NBP1-49602B
DyLight 350 NBP1-49602UV
DyLight 405 NBP1-49602V
DyLight 488 NBP1-49602G
DyLight 550 NBP1-49602R
DyLight 594 NBP1-49602DL594
DyLight 650 NBP1-49602C
DyLight 680 NBP1-49602FR
DyLight 755 NBP1-49602IR
FITC NBP1-49602F
HRP NBP1-49602H
Janelia Fluor 549 NBP1-49602JF549
Janelia Fluor 646 NBP1-49602JF646

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Gene Symbol KDM4A