ASCL2/Mash2 Antibody [Unconjugated]

Western blot shows lysates of JAR human choriocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in extravillous trophoblast. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

A ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.

A ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.

A ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.

A ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta , and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ASCL2/Mash2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ASCL2/Mash2
  Met1-Ala49
  Accession # Q99929
• Specificity: Detects human ASCL2/Mash2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ASCL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASCL2/Mash2 Antibody [Unconjugated]

• achaete-scute complex (Drosophila) homolog-like 2
• achaete-scute complex homolog 2 (Drosophila)
• achaete-scute complex-like 2 (Drosophila)
• ASCL2
• ASH2
• ASH-2
• BHLHA45
• bHLHa45achaete-scute homolog 2
• Class A basic helix-loop-helix protein 45
• HASH2
• HASH2achaete-scute complex-like 2
• mammalian achaete/scute homologue 2
• Mash2

Background

ASCL2 (Achaete-scute like 2; also HASH2, bHLHa45 and Mash2) is a class II member of the HLH family of transcription factors. Its predicted MW is 20 kDa. ASCL2 shows restricted expression, being limited to intestinal Lgr5⁺ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193 amino acids (aa) in length. It contains one poly-Arg motif (aa 36-39), a DNA binding sequence (aa 53-63) and an HLH domain (aa 64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa 1-49, human ASCL2 shares 59% aa identity with mouse Mash2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASCL2/Mash2 Antibody (AF6539)(4)

Publications using AF6539

• Enqi Zheng, Zhun Cai, Wangyong Li, Chuandou Ni, Qian Fang Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma World Journal of Surgical Oncology 2022-08-25 [PMID: 36008864]
• Yukiko Shibahara, Osvaldo Espin-Garcia, James Conner, Jessica Weiss, Mathieu Derouet, Jonathan Allen et al. Intestinal Stem Cell Marker ASCL2 is a Novel Prognostic Predictor in Esophageal Adenocarcinoma Cureus 2022-01-07 [PMID: 35154991]
• Yukiko Shibahara, Osvaldo Espin-Garcia, James Conner, Jessica Weiss, Mathieu Derouet, Jonathan Allen et al. Intestinal Stem Cell Marker ASCL2 is a Novel Prognostic Predictor in Esophageal Adenocarcinoma Cureus 2022-01-07 [PMID: 35154991] (Immunohistochemistry, Human)
• Renaud S, Chakraborty D, Mason C, Rumi M, Vivian J, Soares M OVO-like 1 regulates progenitor cell fate in human trophoblast development. Proc Natl Acad Sci U S A, 2015-10-26;112(45):E6175-84. 2015-10-26 [PMID: 26504231]

Bioinformatics

• Gene Symbol: ASCL2
• Uniprot:
  • Q99929(Human)