Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Analysis of JMJD1A in Cos7 CoCl2 treated lysate.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Staining of JMJD1A in paraffin embedded mouse brain.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - JMJD1A antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Human, mouse and primate. Rat reactivity reported in scientific literature (PMID: 27807143).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
JmjC domain-containing histone demethylation protein 2A
jumonji C domain-containing histone demethylase 2A
jumonji domain containing 1
jumonji domain containing 1A
Jumonji domain-containing protein 1A
Lysine (K)specific Demethylase 3A
Lysine (K)-specific Demethylase 3A
lysine-specific demethylase 3A
testis-specific protein A
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.