Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

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Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Analysis of JMJD1A in Cos7 CoCl2 treated lysate.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Staining of JMJD1A in paraffin embedded mouse brain.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - JMJD1A antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.53 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). [Swiss-Prot Q9Y4C1]
Localization
Nuclear in round spermatids. When spermatids start to elongate, JMJD1A localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
c-Abl Antibody (NB100-78589)
COS-7 Lysate (NB800-PC26)
Publications
Read Publications using
NBP1-49601 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse and primate. Rat reactivity reported in scientific literature (PMID: 27807143).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.53 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM2A
  • JHDM2AJMJD1AJHMD2A
  • JmjC domain-containing histone demethylation protein 2A
  • JMJD1
  • JMJD1A
  • jumonji C domain-containing histone demethylase 2A
  • jumonji domain containing 1
  • jumonji domain containing 1A
  • Jumonji domain-containing protein 1A
  • KDM3A
  • KIAA0742
  • KIAA0742DKFZp686A24246
  • Lysine (K)specific Demethylase 3A
  • Lysine (K)-specific Demethylase 3A
  • lysine-specific demethylase 3A
  • testis-specific protein A
  • TSGA
  • TSGADKFZp686P07111

Background

JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NBP1-49601)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NBP1-49601) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49601AF405
Alexa Fluor 488 Labeled NBP1-49601AF488
Alexa Fluor 647 Labeled NBP1-49601AF647
Alexa Fluor 700 Labeled NBP1-49601AF700
Biotin Labeled NBP1-49601B
DyLight 405 Labeled NBP1-49601V
DyLight 488 Labeled NBP1-49601G
DyLight 550 Labeled NBP1-49601R
DyLight 650 Labeled NBP1-49601C
FITC Labeled NBP1-49601F
HRP Labeled NBP1-49601H

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Bioinformatics

Gene Symbol KDM3A
Entrez
Uniprot