Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Analysis of JMJD1A in Cos7 CoCl2 treated lysate.

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - Staining of JMJD1A in paraffin embedded mouse brain.

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NBP1-49601] - JMJD1A antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.53 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free Summary

• Immunogen: A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). [Swiss-Prot Q9Y4C1]
• Localization: Nuclear in round spermatids. When spermatids start to elongate, JMJD1A localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:5000
• Application Notes: This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  COS-7 Nuclear Hypoxic Induced Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27807143).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.53 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• JHDM2A
• JHDM2AJMJD1AJHMD2A
• JmjC domain-containing histone demethylation protein 2A
• JMJD1
• JMJD1A
• jumonji C domain-containing histone demethylase 2A
• jumonji domain containing 1
• jumonji domain containing 1A
• Jumonji domain-containing protein 1A
• KDM3A
• KIAA0742
• KIAA0742DKFZp686A24246
• Lysine (K)specific Demethylase 3A
• Lysine (K)-specific Demethylase 3A
• lysine-specific demethylase 3A
• testis-specific protein A
• TSGA
• TSGADKFZp686P07111

Background

JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NBP1-49601)(3)

Publications using NBP1-49601

• Chakraborty D, Cui W, Rosario GX et al. HIF-KDM3A-MMP12 regulatory circuit ensures trophoblast plasticity and placental adaptations to hypoxia. PNAS 2016-11-02 [PMID: 27807143] (WB, Rat)
• Kuroki S, Matoba S, Akiyoshi M et al. Epigenetic Regulation of Mouse Sex Determination by the Histone Demethylase Jmjd1a. Science. 2013-09-06 [PMID: 24009392]
• Hanf A Crosstalk between redox regulatory pathways and epigenetic processes in tissue and cell culture models of cardiovascular complications Thesis

Bioinformatics

• Gene Symbol: KDM3A
• Entrez:
  • 55818(Human)
  • 104263(Mouse)
• Uniprot:
  • Q9Y4C1()
  • Q6PCM1(Mouse)