JMJD1C Antibody

Images

 
Western Blot: JMJD1C Antibody [NBP1-77072] - Human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: JMJD1C Antibody [NBP1-77072] - Immunofluorescence of JMJD1C in rat liver tissue with JMJD1C antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: JMJD1C Antibody [NBP1-77072] - Rat liver tissue with JMJD1C antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

JMJD1C Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C. The immunogen is located within amino acids 380 - 430 of JMJD1C.
Specificity
This antibody will not cross-react with JMJD1A or JMJD1B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JMJD1C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
JMJD1C Peptide (NBP1-77072PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77072 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD1C Antibody

  • DKFZp761F0118
  • EC 1.14.11
  • EC 1.14.11.-
  • FLJ14374
  • JHDM2C
  • jumonji domain containing 1C
  • Jumonji domain-containing protein 1C
  • KIAA1380TR-interacting protein 8
  • probable JmjC domain-containing histone demethylation protein 2C
  • RP11-10C13.2
  • thyroid hormone receptor interactor 8
  • thyroid receptor interacting protein 8
  • Thyroid receptor-interacting protein 8
  • TRIP8
  • TRIP-8

Background

The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist. This antibody will not cross-react with JMJD1A or JMJD1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JMJD1C Antibody (NBP1-77072) (0)

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Review for JMJD1C Antibody (NBP1-77072) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77072:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JMJD1C Antibody (NBP1-77072). (Showing 1 - 1 of 1 FAQ).

  1. Would it be possible to know either the exact immunogen sequence or an amino acid range where the immunogen sequence resides?
    • The immunogen lies within amino acids 361-420 of the protein (NCBI Accession: NP_116165). The exact immunogen sequence is proprietary.

Secondary Antibodies

 

Isotype Controls

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Anonymous
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol JMJD1C
Entrez
Uniprot