Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - BSA Free

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) [NBP1-51678] - Western blot analysis using JMJD1A mouse mAb against Hela (1) and HepG2 (2) cell lysates.

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) [NBP1-51678] - JMJD1A antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) [NBP1-51678] - Immunohistochemical analysis of paraffin-embedded colonic cancer tissues (left) and lung cancer tissues (right) using JMJD1A mouse mAb with DAB staining.

Simple Western: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) [NBP1-51678] - Simple Western lane view shows a specific band for JMJD1A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clone: 1E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human JMJD1A expressed in E. coli. [UniProt# Q9Y4C1]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDM3A
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Simple Western 1:500
  • Western Blot 1:500-1:2000
• Application Notes: This JMJD1A (1E12) antibody is useful in Western Blot, ELISA and Immunohistochemistry on paraffin-embedded sections.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:500, apparent MW was 151 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 147 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  HepG2 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12) - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• JHDM2A
• JHDM2AJMJD1AJHMD2A
• JmjC domain-containing histone demethylation protein 2A
• JMJD1
• JMJD1A
• jumonji C domain-containing histone demethylase 2A
• jumonji domain containing 1
• jumonji domain containing 1A
• Jumonji domain-containing protein 1A
• KDM3A
• KIAA0742
• KIAA0742DKFZp686A24246
• Lysine (K)specific Demethylase 3A
• Lysine (K)-specific Demethylase 3A
• lysine-specific demethylase 3A
• testis-specific protein A
• TSGA
• TSGADKFZp686P07111

Background

JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NBP1-51678)(1)

Publication using NBP1-51678

• Kuroki S, Matoba S, Akiyoshi M et al. Epigenetic Regulation of Mouse Sex Determination by the Histone Demethylase Jmjd1a. Science. 2013-09-06 [PMID: 24009392]

Bioinformatics

• Gene Symbol: KDM3A
• Entrez:
  • 55818(Human)
• Uniprot:
  • Q9Y4C1()