LSD1 Antibody - BSA Free

Western Blot: LSD1 Antibody [NBP1-76688] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76688 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: LSD1 Antibody [NBP1-76688] - P815 cell lysate at (A) 1 and (B) 2 ug/ml.

Western Blot: LSD1 Antibody [NBP1-76688] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76417 (0.2 ug/mL), LSD1 NBP1-76688 (1 ug/mL), beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: LSD1 Antibody - BSA Free [NBP1-76688] - Validation of LSD1 in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-LSD1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: LSD1 Antibody - BSA Free [NBP1-76688] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: LSD1 ( 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: LSD1 Antibody - BSA Free [NBP1-76688] - Validation of LSD1 in Human Small Intestine Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine cells labeling LSD1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LSD1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the center of human LSD1. The immunogen is located within amino acids 140 - 190 of LSD1. Amino Acid Squence: QAPPEEENESEPEEPS
• Marker: Nucleus Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM1A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.2-2 ug/ml
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:33831591).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  LSD1 Peptide (NBP1-76688PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LSD1 Antibody - BSA Free

• amine oxidase (flavin containing) domain 2
• AOF2
• AOF2lysine-specific histone demethylase 1
• BHC110
• BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
• Flavin-containing amine oxidase domain-containing protein 2
• KIAA0601KDM1
• LSD1
• LSD1BRAF35-HDAC complex protein BHC110
• lysine (K)-specific demethylase 1
• Lysine (K)specific Demethylase 1A
• Lysine (K)-specific Demethylase 1A
• lysine-specific histone demethylase 1A

Background

Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LSD1 Antibody (NBP1-76688)(1)

Publication using NBP1-76688

• Xie L, Ding N, Zhang H et al. SNF5 promotes IL-1 beta expression via H3K4me1 in atherosclerosis induced by homocysteine The international journal of biochemistry & cell biology 2021-04-05 [PMID: 33831591] (WB, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: KDM1A
• Uniprot:
  • NP_001009999(Human)