Lipin 1 Antibody

Images

 
Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.
Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.8 mg/ml

Lipin 1 Antibody Summary

Immunogen
A synthetic peptide to an internal region (within residues 300-400) of the human LPIN1 protein. [Swiss-Prot# Q14693]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0-2.0 ug/ml
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This LPIN1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot analysis, where a and is seen at ~97 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Lipin 1 Overexpression Lysate
Lipin 1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB110-57150 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse, rat and human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lipin 1 Antibody

  • DKFZp781P1796
  • EC 3.1.3.18
  • EC 3.1.3.4
  • EC 5.4.2.7
  • KIAA0188phosphatidate phosphatase LPIN1
  • Lipin 1
  • lipin-1
  • LPIN1
  • PAP1

Background

LPIN1 (phosphatidate phosphatase LPIN1) is expressed most abundantly in adipocytes and skeletal muscles wherein it play two major roles (i) in the cytoplasm, LPIN1 acts as a phosphatidate phosphatase 1 (PAP1) enzyme converting phosphatidic acid to diacylglycerol which is the precursor of both triglycerides and phospholipids (ii) in the nucleus, LPIN1 functions as a transcriptional co-activator through interaction with transcription factors which regulate the expression of genes involved in energy pathways. It can interact with MEF2C and LPIN1 interction with PPARA as well as PPARGC1A leads to a complex formation that modulates gene transcription. Three LPIN-1 protein isoforms are generated by alternative splicing, specifically involved in either PAP1 activity, transcriptional function or lipid droplets biogenesis. Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipin 1 Antibody (NB110-57150)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Mouse
(2)
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Product General Protocols

View specific protocols for Lipin 1 Antibody (NB110-57150): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lipin 1 Antibody (NB110-57150). (Showing 1 - 1 of 1 FAQs).

  1. I want to use Lpin1 antibody for mouse liver paraffin sections (tumor also). What is your opinion about success of this staining? What is the suitable dilution for IHC?
    • NB110-57150 has been tested and validated for use in human, mouse and rat. It is therefore guaranteed to work in those species and should be a good antibody to use in your mouse sections. The recommended dilution for IHC on paraffin embedded sections is 1:100 to 1:250.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-57150AF350
Alexa Fluor 405 NB110-57150AF405
Alexa Fluor 488 NB110-57150AF488
Alexa Fluor 532 NB110-57150AF532
Alexa Fluor 594 NB110-57150AF594
Alexa Fluor 647 NB110-57150AF647
Alexa Fluor 700 NB110-57150AF700
Alexa Fluor 750 NB110-57150AF750
Biotin NB110-57150B
DyLight 350 NB110-57150UV
DyLight 405 NB110-57150V
DyLight 488 NB110-57150G
DyLight 550 NB110-57150R
DyLight 594 NB110-57150DL594
DyLight 650 NB110-57150C
DyLight 680 NB110-57150FR
DyLight 755 NB110-57150IR
FITC NB110-57150F
HRP NB110-57150H
Janelia Fluor 549 NB110-57150JF549
Janelia Fluor 646 NB110-57150JF646

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Bioinformatics

Gene Symbol LPIN1