Lipin 3 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipin 3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Lipin 3
  Thr428-Arg529
  Accession # Q99PI4
• Specificity: Detects mouse Lipin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Lipin 3 is observed and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LPIN3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipin 3 Antibody [Unconjugated]

• dJ450M14.2
• dJ450M14.3
• dJ620E11.2
• EC 3.1.3.4
• Lipin 3
• lipin 3-like
• lipin-3
• Lipin-3-like
• LIPN3L
• LPIN3
• phosphatidate phosphatase LPIN3
• SMP2

Background

Mouse Lipin 3 is a 94 kDa member of the small Lipin family of nuclear proteins. It is primarily expressed in the GI tract and may play a role in DAG production or membrane synthesis. Lipin 3 is 848 amino acids (aa) in length. It shows conserved N- and C-terminal domains that flank an NLS between aa 135‑144. Lipin 3 has modest phosphatidate phosphatase activity. Two potential isoforms exist; one shows a deletion of aa 507‑537, while the other shows a 10 aa insertion between Glu 506 and Ser 507. Over aa 428‑549, mouse Lipin 3 shares 84% and 96% aa sequence identity with human and rat Lipin 3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LPIN3
• Uniprot:
  • Q99PI4()