PAPOLA Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PAPOLA Antibody [NBP1-87345] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PAPOLA Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAPOLA
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PAPOLA Protein (NBP1-87345PEP)

Reactivity Notes

Mouse (88%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PAPOLA Antibody

  • EC 2.7.7
  • EC 2.7.7.19
  • PAP
  • PAP-alpha
  • PAPMGC5378
  • PAPOLA
  • poly(A) polymerase alpha
  • Polynucleotide adenylyltransferase alpha

Background

PAPOLA is a polymerase that creates the 3' poly(A) tail of mRNA's. It is also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. PAPOLA may acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PAPOLA