Lipin 1 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit

Lipin 1 Antibody [FITC] Summary

Immunogen
A synthetic peptide to an internal region (within residues 300-400) of the human LPIN1 protein. [Swiss-Prot# Q14693]
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Gene
LPIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
FITC
Format
FITC (A=495, E=519)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1.0-2.0 ug/ml
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This LPIN1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot analysis, where a and is seen at ~97 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse, rat and human.

Alternate Names for Lipin 1 Antibody [FITC]

  • DKFZp781P1796
  • EC 3.1.3.18
  • EC 3.1.3.4
  • EC 5.4.2.7
  • KIAA0188phosphatidate phosphatase LPIN1
  • Lipin 1
  • lipin-1
  • LPIN1
  • PAP1

Background

LPIN1 (phosphatidate phosphatase LPIN1) is expressed most abundantly in adipocytes and skeletal muscles wherein it play two major roles (i) in the cytoplasm, LPIN1 acts as a phosphatidate phosphatase 1 (PAP1) enzyme converting phosphatidic acid to diacylglycerol which is the precursor of both triglycerides and phospholipids (ii) in the nucleus, LPIN1 functions as a transcriptional co-activator through interaction with transcription factors which regulate the expression of genes involved in energy pathways. It can interact with MEF2C and LPIN1 interction with PPARA as well as PPARGC1A leads to a complex formation that modulates gene transcription. Three LPIN-1 protein isoforms are generated by alternative splicing, specifically involved in either PAP1 activity, transcriptional function or lipid droplets biogenesis. Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Lipin 1 NB110-57150F

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol LPIN1
Entrez
Uniprot

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