PAG3 Antibody

Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. 1.0 ug/ml.

• Reactivity: Hu, Mu, Rt, Ma-Op, Pm, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PAG3 Antibody Summary

• Immunogen: A portion of amino acids 700-750 of human ASAP2 was used as the immunogen.
• Predicted Species: Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASAP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0-2.0 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_c and CRA_e), chimpanzee and monkey, 86% homologous in horse, 83% homologous in mouse, rat, and cow, and 73% homologous in dog.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for PAG3 Antibody

• arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
• ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
• centaurin, beta 3
• CENTB3
• DDEF2FLJ42910
• development and differentiation enhancing factor 2
• Development and differentiation-enhancing factor 2
• KIAA0400AMAP2
• Pap-alpha
• PAPPAG3
• Paxillin-associated protein with ARF GAP activity 3
• PYK2 C terminus-associated protein
• Pyk2 C-terminus-associated protein
• SHAG1

Background

PAG3 encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASAP2
• Uniprot:
  • O43150(Human)