PAPOLG Antibody

Immunoprecipitation: PAPOLG Antibody [NBP1-30060] - WCL from HeLa cells. NBP1-30060 used for IP at 10 mcg/mg lysate. PAP-gamma was also IPed by rabbit anti-PAP-gamma antibody NBP1-30061. For blotting IPed PAP-gamma, NBP1-30061 was used at 1 mcg/ml.

Knockdown Validated: PAPOLG Antibody [NBP1-30060] - An internal A-tract does not promote splicing in the absence of a 3'-terminal poly(A) tail. Western blot analysis of PAPalpha and PAPgamma (PAPOLG) proteins in cells treated with control or PAPalpha- and PAPgamma-specific siRNAs. Histone H3 is shown as a loading control. Image collected and cropped by CiteAb from the following publication (//mcb.asm.org/content/35/13/2218), licensed under a CC-BY licence.

• Reactivity: Hu
• Applications: WB, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PAPOLG Antibody Summary

• Immunogen: maps to a region between residue 625 and 675 of human poly(A) polymerase gamma using the numbering given in entry NP_075045.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPOLG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ot 15 ug/mg lysate
  • Knockdown Validated
  • Western Blot
• Application Notes: This antibody is useful in Immunoprecipitation, Use in Western blot reported in scientific literature (PMID 25896913)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAPOLG Antibody

• EC 2.7.7.19
• FLJ11805
• FLJ12972
• FLJ13482
• FLJ14187
• MGC133307
• MGC133308
• neo-PAP
• Neo-poly(A) polymerase
• nuclear poly(A) polymerase gamma
• PAP2
• PAPG
• PAP-gamma
• poly(A) polymerase gamma
• Polynucleotide adenylyltransferase gamma
• SRP RNA 3' adenylating enzyme/pap2
• SRP RNA 3'-adenylating enzyme

Background

PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAPOLG Antibody (NBP1-30060)(1)

Publication using NBP1-30060

• Muniz L, Davidson L, West S. Poly(A) polymerase and the nuclear poly(A) binding protein, PABPN1, coordinate the splicing and degradation of a subset of human pre-mRNAs Mol. Cell. Biol. 2015-04-20 [PMID: 25896913] (WB, Human)

Bioinformatics

• Gene Symbol: PAPOLG