PAPOLG Antibody - BSA Free

Immunoprecipitation: PAPOLG Antibody [NBP1-30060] - WCL from HeLa cells. NBP1-30060 used for IP at 10 mcg/mg lysate. PAP-gamma was also IPed by rabbit anti-PAP-gamma antibody NBP1-30061. For blotting IPed PAP-gamma, NBP1-30061 was used at 1 mcg/ml.

Western Blot: PAPOLG Antibody [NBP1-30060] - An internal A-tract does not promote splicing in the absence of a 3'-terminal poly(A) tail. Western blot analysis of PAPalpha and PAPgamma (PAPOLG) proteins in cells treated with control or PAPalpha- and PAPgamma-specific siRNAs. Histone H3 is shown as a loading control. Image collected and cropped by CiteAb from the following publication (//mcb.asm.org/content/35/13/2218), licensed under a CC-BY license.

Western Blot: PAPOLG Antibody [NBP1-30060] - An internal A-tract does not promote splicing in the absence of a 3′-terminal poly(A) tail. (A) Schematics of beta WT & beta AnMEN beta δ plasmids, with the positions of the internal A-tracts within exon 3 indicated. Other symbols are the same as in Fig. 1A. (B) RNA immunoprecipitation to assay the level of unspliced beta -globin RNAs bound by PABPN1 in HeLa cells transiently transfected with the beta WT, beta MEN beta δ, beta A20MEN beta δ, beta A40MEN beta δ, or beta A60MEN beta δ construct. Values are expressed as % input following normalization to PSMB3 pre-mRNA levels. (C) RT-qPCR analysis of beta -globin exon 2 & 3 splicing in HeLa cells transiently transfected with the beta WT, beta MEN beta δ, beta A20MEN beta δ, beta A40MEN beta δ, or beta A60MEN beta δ construct. The level of each RNA species was quantitated relative to that recovered from cells transfected with the beta WT construct, which was given a value of 1. (D) Western blot analysis of PAP alpha & PAP gamma proteins in cells treated with control or PAP alpha - & PAP gamma -specific siRNAs. Histone H3 is shown as a loading control. (E) RT-qPCR analysis of exon 2 & 3 splicing in beta WT & beta A46δ cells treated with control or PAP alpha - & PAP gamma -specific siRNAs. The level of each RNA species is shown as the fold change compared to the level in control siRNA-treated cells after normalization to GAPDH mRNA. All error bars represent standard deviations for at least three biological replicates. *, P < 0.05; **, P < 0.01. US, unspliced; Spl, spliced. Image collected & cropped by CiteAb from the following publication (//www.tandfonline.com/doi/full/10.1128/MCB.00123-15), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PAPOLG Antibody - BSA Free Summary

• Immunogen: maps to a region between residue 625 and 675 of human poly(A) polymerase gamma using the numbering given in entry NP_075045.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPOLG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ot 15 ug/mg lysate
  • Knockdown Validated
  • Western Blot Reported in scientific literature (PMID 25896913)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAPOLG Antibody - BSA Free

• EC 2.7.7.19
• FLJ11805
• FLJ12972
• FLJ13482
• FLJ14187
• MGC133307
• MGC133308
• neo-PAP
• Neo-poly(A) polymerase
• nuclear poly(A) polymerase gamma
• PAP2
• PAPG
• PAP-gamma
• poly(A) polymerase gamma
• Polynucleotide adenylyltransferase gamma
• SRP RNA 3' adenylating enzyme/pap2
• SRP RNA 3'-adenylating enzyme

Background

PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAPOLG Antibody (NBP1-30060)(1)

Publication using NBP1-30060

• Muniz L, Davidson L, West S. Poly(A) polymerase and the nuclear poly(A) binding protein, PABPN1, coordinate the splicing and degradation of a subset of human pre-mRNAs Mol. Cell. Biol. 2015-04-20 [PMID: 25896913] (WB, Human)

Bioinformatics

• Gene Symbol: PAPOLG