PAPOLG Antibody

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Immunoprecipitation: PAPOLG Antibody [NBP1-30060] - WCL from HeLa cells. NBP1-30060 used for IP at 10 mcg/mg lysate. PAP-gamma was also IPed by rabbit anti-PAP-gamma antibody NBP1-30061. For blotting IPed PAP-gamma, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PAPOLG Antibody Summary

Immunogen
maps to a region between residue 625 and 675 of human poly(A) polymerase gamma using the numbering given in entry NP_075045.2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAPOLG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunoprecipitation 10 ot 15 ug/mg lysate
Application Notes
This antibody is useful in Immunoprecipitation, Use in Western blot reported in scientific literature (PMID 25896913)
Publications
Read Publication using
NBP1-30060 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAPOLG Antibody

  • EC 2.7.7.19
  • FLJ11805
  • FLJ12972
  • FLJ13482
  • FLJ14187
  • MGC133307
  • MGC133308
  • neo-PAP
  • Neo-poly(A) polymerase
  • nuclear poly(A) polymerase gamma
  • PAP2
  • PAPG
  • PAP-gamma
  • poly(A) polymerase gamma
  • Polynucleotide adenylyltransferase gamma
  • SRP RNA 3' adenylating enzyme/pap2
  • SRP RNA 3'-adenylating enzyme

Background

PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAPOLG Antibody (NBP1-30060)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PAPOLG