Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein, CF


Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (1324-WN).

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein, CF Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40-43 kDa, reducing conditions
Read Publications using
7150-RS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein, CF

  • Cristin 3
  • FLJ40906Roof plate-specific spondin-1
  • HRspo1
  • roof plate-specific spondin
  • RSPO
  • RSPO1
  • RSpondin 1
  • R-Spondin 1
  • R-spondin homolog (Xenopus laevis)
  • R-spondin1
  • R-spondin-1


R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11). Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.
  1. Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  9. Kim, K.-A. et al. (2005) Science 309:1256.
  10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: IHC, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: ICC, Simple Western, WB
Species: Bv, Ma, Hu, Mu
Applications: ChIP, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: WB
Species: Hu
Applications: BA
Species: Hu, Mu, Po
Applications: Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Flow, WB
Species: Hu
Applications: BA
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: BA, BA

Publications for R-Spondin 1 (7150-RS/CF)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.

Filter By Application
Cell Culture
All Applications
Filter By Species
All Species
Showing Publications 1 - 8 of 8.
Publications using 7150-RS/CF Applications Species
Z Fu, JW Dean, L Xiong, MW Dougherty, KN Oliff, ZE Chen, C Jobin, TJ Garrett, L Zhou Mitochondrial transcription factor A in RORgammat+ lymphocytes regulate small intestine homeostasis and metabolism Nature Communications, 2021;12(1):4462. 2021 [PMID: 34294718] (Bioassay, Mouse) Bioassay Mouse
K Dunbar, A Valanciute, A Lima, PF Vinuela, T Jamieson, V Rajasekara, J Blackmur, AM Ochocka-Fo, A Guazzelli, P Cammareri, MJ Arends, OJ Sansom, KB Myant, SM Farrington, MG Dunlop, FVN Din Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1 Cell Mol Gastroenterol Hepatol, 2020;0(0):. 2020 [PMID: 32971322] (Cell Culture, Mouse) Cell Culture Mouse
MW Dougherty, O Kudin, M Mühlbauer, J Neu, RZ Gharaibeh, C Jobin Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites BMC Microbiol., 2020;20(1):205. 2020 [PMID: 32652929] (Bioassay, Mouse) Bioassay Mouse
Y Cai, G Zhu, S Liu, Z Pan, M Quintero, CJ Poole, C Lu, H Zhu, B Islam, JV Riggelen, D Browning, K Liu, R Blumberg, N Singh, H Li Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation Cell Discov, 2019;5(0):7. 2019 [PMID: 30701081] (Bioassay, Mouse) Bioassay Mouse
Y Eriguchi, K Nakamura, Y Yokoi, R Sugimoto, S Takahashi, D Hashimoto, T Teshima, T Ayabe, ME Selsted, AJ Ouellette Essential role of IFN-? in T cell-associated intestinal inflammation JCI Insight, 2018;3(18):. 2018 [PMID: 30232288] (Bioassay, Mouse) Bioassay Mouse
G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29559533] (Bioassay, Human) Bioassay Human
X Yin, H Yi, L Wang, W Wu, X Wu, L Yu RSPOs facilitated HSC activation and promoted hepatic fibrogenesis Oncotarget, 2016;7(39):63767-63778. 2016 [PMID: 27572318] (Bioassay, Mouse) Bioassay Mouse
Marie-Isabelle Garcia Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 2016;143(9):1452-63. 2016 [PMID: 26989172] (Bioassay, Mouse) Bioassay Mouse

Reviews for R-Spondin 1 (7150-RS/CF) (0)

There are no reviews for R-Spondin 1 (7150-RS/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for R-Spondin 1 (7150-RS/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional R-Spondin 1 Products

Bioinformatics Tool for R-Spondin 1 (7150-RS/CF)

Discover related pathways, diseases and genes to R-Spondin 1 (7150-RS/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for R-Spondin 1 (7150-RS/CF)

Discover more about diseases related to R-Spondin 1 (7150-RS/CF).

Pathways for R-Spondin 1 (7150-RS/CF)

View related products by pathway.

PTMs for R-Spondin 1 (7150-RS/CF)

Learn more about PTMs related to R-Spondin 1 (7150-RS/CF).

Blogs on R-Spondin 1

There are no specific blogs for R-Spondin 1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein, CF and receive a gift card or discount.


Gene Symbol Rspo1