Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 ...read more
Western Blot: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.
This Bmi1 Antibody (LLBmi1-1) is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation and Western blot, where a band can be seen at approximately 42 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-96140 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for BMI-1 Antibody (LLBmi1-1)
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
B lymphoma Mo-MLV insertion region 1 homolog
BMI1 polycomb ring finger oncogene
murine leukemia viral (bmi-1) oncogene homolog
polycomb complex protein BMI-1
polycomb group protein Bmi1
polycomb group ring finger 4
RING finger protein 51
RNF51Polycomb group RING finger protein 4
Bmi1 is a member of the polycomb group (PcG) multiprotein PRC1 complex which functions to control axial skeleton development through Hox gene repression. Bmi1 plays an important role in repressing the transcription of the p16Ink4a and p19Arf genes and is required to maintain the proliferative and self-renewal properties of adult hematopoietic and neural stem cells. Bmi1 is an oncogene whose overexpression has been associated with many types of human tumors, including those in breast, colorectal, prostate, and non-small cell lung cancers. As seen in many recent publications, Bmi1 antibodies can serve as useful research tools to study Bmi1 and its effect on tumor proliferation and tumorigenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.