BMI-1 Antibody (LLBmi1-1)

Images

 
Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 ...read more
Western Blot: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IP
Clone
LLBmi1-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

BMI-1 Antibody (LLBmi1-1) Summary

Immunogen
Human Bmi1 protein [Swiss-Prot# P35226]
Localization
Nucleus. Cytoplasm.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BMI1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This Bmi1 Antibody (LLBmi1-1) is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation and Western blot, where a band can be seen at approximately 42 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-96140 in the following applications:

Publications
Read Publications using
NBP1-96140 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BMI-1 Antibody (LLBmi1-1)

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

Bmi1 is a member of the polycomb group (PcG) multiprotein PRC1 complex which functions to control axial skeleton development through Hox gene repression. Bmi1 plays an important role in repressing the transcription of the p16Ink4a and p19Arf genes and is required to maintain the proliferative and self-renewal properties of adult hematopoietic and neural stem cells. Bmi1 is an oncogene whose overexpression has been associated with many types of human tumors, including those in breast, colorectal, prostate, and non-small cell lung cancers. As seen in many recent publications, Bmi1 antibodies can serve as useful research tools to study Bmi1 and its effect on tumor proliferation and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMI-1 Antibody (NBP1-96140)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


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ChIP
(1)
WB
(1)
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Human
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Mouse
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Review for BMI-1 Antibody (NBP1-96140) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-96140:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot BMI-1 NBP1-96140
Enlarge
reviewed by:
Anonymous
WB Human 06/30/2015
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman
LotLot B-1

Blocking

Blocking Details4% BSA in TBST for 1h at room temperature

Primary Anitbody

Dilution Ratio1:1000 in TBST + 1%BSA, overnight, 4*C

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#sc-2302
Secondary Concentration1:10,000

Details

Detection NotesECL

Product General Protocols

View specific protocols for BMI-1 Antibody (NBP1-96140): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for BMI-1 Antibody (NBP1-96140). (Showing 1 - 1 of 1 FAQs).

  1. Can you provide the ChIP results for this antibody? I am looking for good Bmi1 antibodies for ChIP.
    • We obtained our ChIP verification information from the publication listed on the data sheet. Please refer to Courel et al. Dev Dyn. 2008 May;237(5):1232-42 PMID 18366140.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-96140AF405
Alexa Fluor 488 Labeled NBP1-96140AF488
Alexa Fluor 647 Labeled NBP1-96140AF647
Alexa Fluor 700 Labeled NBP1-96140AF700
Biotin Labeled NBP1-96140B
DyLight 350 Labeled NBP1-96140UV
DyLight 405 Labeled NBP1-96140V
DyLight 488 Labeled NBP1-96140G
DyLight 550 Labeled NBP1-96140R
DyLight 650 Labeled NBP1-96140C
DyLight 680 Labeled NBP1-96140FR
DyLight 755 Labeled NBP1-96140IR
FITC Labeled NBP1-96140F
HRP Labeled NBP1-96140H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/30/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol BMI1
Entrez
Uniprot