CD24 Antibody (ML5)

Images

 
Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) [NB100-77903] - MCF-7 breast cancer cells.
Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.
Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ML5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CD24 Antibody (ML5) Summary

Immunogen
The immunogen was the full length version of the protein: CD24 (ML5)
Specificity
CD24 (ML5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD24
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence <0.125 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5-25 ug/ml
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD24 Antibody (ML5)

  • CD 24
  • CD24 antigen (small cell lung carcinoma cluster 4 antigen)
  • CD24 antigen
  • CD24 molecule
  • CD24
  • CD24A
  • CD24Asignal transducer CD24
  • FLJ22950
  • FLJ43543
  • MGC75043
  • Small cell lung carcinoma cluster 4 antigen

Background

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD24 Antibody (NB100-77903) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77903AF350
Alexa Fluor 405 NB100-77903AF405
Alexa Fluor 488 NB100-77903AF488
Alexa Fluor 532 NB100-77903AF532
Alexa Fluor 594 NB100-77903AF594
Alexa Fluor 647 NB100-77903AF647
Alexa Fluor 700 NB100-77903AF700
Alexa Fluor 750 NB100-77903AF750
Allophycocyanin NB100-77903APC
DyLight 350 NB100-77903UV
DyLight 405 NB100-77903V
DyLight 488 NB100-77903G
DyLight 550 NB100-77903R
DyLight 594 NB100-77903DL594
DyLight 650 NB100-77903C
DyLight 680 NB100-77903FR
DyLight 755 NB100-77903IR
PE NB100-77903PE
PerCP NB100-77903PCP

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Bioinformatics

Gene Symbol CD24
Entrez
Uniprot