DCLK1 Antibody - BSA Free

Images

 
Western Blot: DCLK1 Antibody [NBP1-77127] - Analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: DCLK1 Antibody [NBP1-77127] - Staining of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 20 ug/mL.
Immunohistochemistry: DCLK1 Antibody [NBP1-77127] - Staining of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 5 ug/mL.
Western Blot: DCLK1 Antibody [NBP1-77127] - Human brain tissue lysate with DCAMILK1 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: DCLK1 Antibody [NBP1-77127] - Rat brain tissue with DCAMLK1 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

DCLK1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK1. The immunogen is located within the last 50 amino acids of DCLK1. Amino Acid Squence: IRRGRFSDEDATRM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DCLK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DCLK1 Protein (NBP1-77127PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DCLK1 Antibody - BSA Free

  • CL1
  • CLICK1
  • DCAMKL1
  • DCAMKL1CLICK1
  • DCDC3A
  • DCDC3ACL1
  • DCLK
  • DCLK1
  • doublecortin and CaM kinase-like 1
  • Doublecortin domain-containing protein 3A
  • doublecortin-like kinase 1Doublecortin-like and CAM kinase-like 1
  • EC 2.7.11
  • KIAA0369EC 2.7.11.1
  • serine/threonine-protein kinase DCLK1

Background

DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-77127

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Bioinformatics

Gene Symbol DCLK1
Uniprot