Leptin R Antibody (JA73-01)

Images

 
Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of Leptin Receptor on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: Leptin R Antibody (JA73-01) [NBP2-66931] - Staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Flow Cytometry: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary ...read more
Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Western blot analysis of Leptin R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JA73-01
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Leptin R Antibody (JA73-01) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human Leptin R aa 1041-1090 / 1165. (SwissProt: P48357 Human; SwissProt: P48356 Mouse; SwissProt: Q62959 Rat)
Localization
Secreted and Cell membrane. Basolateral cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LEPR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Leptin R Antibody (JA73-01)

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295

Background

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for Leptin R Antibody (NBP2-66931)

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Bioinformatics

Gene Symbol LEPR