AgRP/ART Antibody

AgRP/ART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AgRP/ART Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP/ART
  Ala21-Thr132
  Accession # O00253
• Specificity: Detects human AgRP/ART in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP/ART is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGRP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AgRP/ART Antibody

• agouti related protein homolog (mouse)
• agouti-related protein
• AgRP
• Agrt
• AGRTMGC118963
• ART
• ARTagouti (mouse) related protein
• ASIP2

Background

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).

References

1. Martin, N.M. et al. (2006) Peptides 27:333.
2. Fan, W. et al. (1997) Nature 385:165.
3. Ollmann, M.M. et al. (1997) Science 278:135.
4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
5. Shutter, J.R. et al. (1997) Genes Develop. 11:593.
6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
11. Small, C.K. et al. (2001) Diabetes 50:248.
12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265 - 266:108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AgRP/ART Antibody (AF704)(2)

Publications using AF704

• Sweet, SR;Biddinger, JE;Zimmermann, JB;Yu, GL;Simerly, RB; Early perturbations to fluid homeostasis alter development of hypothalamic feeding circuits with context-specific changes in ingestive behavior bioRxiv : the preprint server for biology 2024-10-26 [PMID: 39484367] (Immunohistochemistry, Mouse)
• Minghao Liu, Diane Hamele-Bena, John Ausiello, Gabrielle Page-Wilson Ectopic ACTH Syndrome Emerging 5 Years after the Diagnosis of Neuroendocrine Tumor Case Reports in Endocrinology 2019-05-28 [PMID: 31275666]

Bioinformatics

• Gene Symbol: AGRP
• Uniprot:
  • O00253()