LEDGF Antibody

Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.

• Reactivity: Hu, Bv, Fe
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

LEDGF Antibody Summary

• Description: Novus Biologicals Rabbit LEDGF Antibody (NB110-40561) is a polyclonal antibody validated for use in WB and IP. Anti-LEDGF Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between 480 and the C-terminus(residue 530) of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).
• Predicted Species: Bovine (100%), Feline (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LEDGF Antibody

• CLL-associated antigen KW-7
• Dense fine speckles 70 kDa protein
• DFS 70
• DFS70
• LEDGF
• LEDGFDFS 70
• Lens epithelium-derived growth factor
• MGC74712
• p52
• p75
• PAIP
• PC4 and SFRS1 interacting protein 1
• PC4 and SFRS1 interacting protein 2
• PC4 and SFRS1-interacting protein
• PSIP1
• PSIP2
• transcriptional coactivator p52/p75
• Transcriptional coactivator p75/p52

Background

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LEDGF Antibody (NB110-40561)(7)

Publications using NB110-40561

• Nakamura T, Campbell JR, Moore AR et al. Development and validation of a cell-based assay system to assess human immunodeficiency virus type 1 integrase multimerization J. Virol. Methods 2016-07-26 [PMID: 27474494]
• Desimmie BA, Humbert M, Lescrinier E et al. Phage display-directed discovery of LEDGF/p75 binding cyclic peptide inhibitors of HIV replication. Mol Ther 2012-11-01 [PMID: 22828501]
• Pradeepa MM, SutherlHG, Ule J et al. Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing. PLoS Genet 2012-01-01 [PMID: 22615581]
• Leoh LS, van Heertum B, De Rijck J et al. The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity. Mol Cancer Res 2012-03-01 [PMID: 22275515]
• Gijsbers R, Vets S, De Rijck J et al. Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting. J Biol Chem 2011-12-01 [PMID: 21987578]
• Basu A, Drame A, Munoz R et al. Pathway specific gene expression profiling reveals oxidative stress genes potentially regulated by transcription co-activator LEDGF/p75 in prostate cancer cells. Prostate. 2011-07-27 [PMID: 21796653] (WB, Human)
• De Rijck J, Bartholomeeusen K, Ceulemans H et al. High-resolution profiling of the LEDGF/p75 chromatin interaction in the ENCODE region. Nucleic Acids Res 2010-10-01 [PMID: 20484370]

Bioinformatics

• Gene Symbol: PSIP1
• Entrez:
  • 11168(Human)
• Uniprot:
  • O75475()