LEDGF Antibody

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Independent Antibodies: Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit ...read more

Product Details

Summary
Reactivity Hu, Bv, FeSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

LEDGF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between 480 and the C-terminus(residue 530) of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).
Predicted Species
Feline (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This antibody is useful for Western Blot and Immunoprecipitation. Optimal dilution to be determined by the researcher.
Positive Control
LEDGF Lysate (NBP2-65785)
Publications
Read Publications using
NB110-40561 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LEDGF Antibody

  • CLL-associated antigen KW-7
  • Dense fine speckles 70 kDa protein
  • DFS 70
  • DFS70
  • LEDGF
  • LEDGFDFS 70
  • Lens epithelium-derived growth factor
  • MGC74712
  • p52
  • p75
  • PAIP
  • PC4 and SFRS1 interacting protein 1
  • PC4 and SFRS1 interacting protein 2
  • PC4 and SFRS1-interacting protein
  • PSIP1
  • PSIP2
  • transcriptional coactivator p52/p75
  • Transcriptional coactivator p75/p52

Background

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for LEDGF Antibody (NB110-40561)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-40561 Applications Species
Nakamura T, Campbell JR, Moore AR et al. Development and validation of a cell-based assay system to assess human immunodeficiency virus type 1 integrase multimerization J. Virol. Methods 2016 Jul 26 [PMID: 27474494]
Desimmie BA, Humbert M, Lescrinier E et al. Phage display-directed discovery of LEDGF/p75 binding cyclic peptide inhibitors of HIV replication. Mol Ther 2012 Nov [PMID: 22828501]
Pradeepa MM, SutherlHG, Ule J et al. Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing. PLoS Genet 2012 [PMID: 22615581]
Leoh LS, van Heertum B, De Rijck J et al. The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity. Mol Cancer Res 2012 Mar [PMID: 22275515]
Gijsbers R, Vets S, De Rijck J et al. Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting. J Biol Chem 2011 Dec [PMID: 21987578]
Basu, A, Drame, A, Munoz, R et al. Pathway specific gene expression profiling reveals oxidative stress genes potentially regulated by transcription co-activator LEDGF/p75 in prostate cancer cells. Prostate 2012 [PMID: 21796653] (WB, Human) WB Human
De Rijck J, Bartholomeeusen K, Ceulemans H et al. High-resolution profiling of the LEDGF/p75 chromatin interaction in the ENCODE region. Nucleic Acids Res 2010 Oct [PMID: 20484370]

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSIP1
Entrez
Uniprot