LAMP-2/CD107b Antibody

Images

 
Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 25ug of NRK cell lysates.
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP2 ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.
Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 10ug of HeLa (left panel), NIH/3T3 (middle panel) and NRK (right panel) whole cell lysates.
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2 (green) in NIH/3T3 cells.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human tonsils.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Normal deparaffinized Human placenta tissue tissues.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LAMP-2/CD107b Antibody Summary

Immunogen
Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411)
Localization
Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Marker
Late Endosome / Lysosome marker
Specificity
LAMP 2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:10-1:500
Publications
Read Publications using
NB300-591 in the following applications:

Reactivity Notes

Rat reported in literature (PMID: 15689499)..

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LAMP-2/CD107b Antibody

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMPB
  • LGP110
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome-associated membrane protein-2 (LAMP-2) is an ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-2/CD107b Antibody (NB300-591)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for LAMP-2/CD107b Antibody (NB300-591)

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Diseases for LAMP-2/CD107b Antibody (NB300-591)

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Blogs on LAMP-2/CD107b.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

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Bioinformatics

Gene Symbol LAMP2
Entrez