Cathepsin B Antibody [Unconjugated]

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Cathepsin B was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Goat Anti-Mouse Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF965) overnight at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Cathepsin B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
His18-Phe339
Accession # P10605
Specificity
Detects mouse Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse Cathepsin H is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.33 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF965 in the following applications:

Publications
Read Publications using
AF965 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin B Antibody [Unconjugated]

  • APP secretase
  • APPS
  • Cathepsin B
  • Cathepsin B1
  • CPSBamyloid precursor protein secretase
  • CTSB
  • cysteine protease
  • EC 3.4.22
  • EC 3.4.22.1

Background

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2‑4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution in human and animal tumors, Cathepsin B may also have a role in invasion and metastasis (5). In addition to the lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (5).
  1. Mort, J.S. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 1079, Academic Press, San Diego.
  2. Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
  3. Jutras, I. and T.L. Reudelhuber (1998) FEBS Lett. 443:48.
  4. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  5. Berquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin B Antibody (AF965)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(3)
IHC
(2)
Western Blot
(12)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
Rat
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF965 Applications Species
E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022;13(1):4848. 2022 [PMID: 35977928] (Western Blot, Mouse) Western Blot Mouse
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35245440] (Western Blot, Mouse) Western Blot Mouse
H Iwama, S Mehanna, M Imasaka, S Hashidume, H Nishiura, KI Yamamura, C Suzuki, Y Uchiyama, E Hatano, M Ohmuraya Cathepsin B and D deficiency in the mouse pancreas induces impaired autophagy and chronic pancreatitis Scientific Reports, 2021;11(1):6596. 2021 [PMID: 33758261] (IHC, Mouse) IHC Mouse
L Bonet-Ponc, A Beilina, CD Williamson, E Lindberg, JH Kluss, S Saez-Atien, N Landeck, R Kumaran, A Mamais, CKE Bleck, Y Li, MR Cookson LRRK2 mediates tubulation and vesicle sorting from lysosomes Sci Adv, 2020;6(46):. 2020 [PMID: 33177079] (ICC, Human) ICC Human
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020;8(1):163. 2020 [PMID: 33028409] (Western Blot, Mouse) Western Blot Mouse
J Selkrig, N Li, A Hausmann, MSJ Mangan, M Zietek, A Mateus, J Bobonis, A Sueki, H Imamura, B El Debs, G Sigismondo, BI Florea, HS Overkleeft, N Kopitar-Je, B Turk, P Beltrao, MM Savitski, E Latz, WD Hardt, J Krijgsveld, A Typas Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection Nat Microbiol, 2020;0(0):. 2020 [PMID: 32514074] (Western Blot, Mouse) Western Blot Mouse
J Schwenck, A Maurer, B Fehrenbach, R Mehling, P Knopf, N Mucha, D Haupt, K Fuchs, CM Griessinge, D Bukala, J Holstein, M Schaller, IG Menendez, K Ghoreschi, L Quintanill, M Gütschow, S Laufer, T Reinheckel, M Röcken, H Kalbacher, BJ Pichler, M Kneilling Cysteine-type cathepsins promote the effector phase of acute cutaneous delayed-type hypersensitivity reactions Theranostics, 2019;9(13):3903-3917. 2019 [PMID: 31281521] (Western Blot, Mouse) Western Blot Mouse
T Farfel-Bec, JC Roney, XT Cheng, S Li, SR Cuddy, ZH Sheng Neuronal Soma-Derived Degradative Lysosomes Are Continuously Delivered to Distal Axons to Maintain Local Degradation Capacity Cell Rep, 2019;28(1):51-64.e4. 2019 [PMID: 31269450] (ICC, Mouse) ICC Mouse
JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice Mol Neurodegener, 2018;13(1):48. 2018 [PMID: 30180904] (Western Blot, Mouse) Western Blot Mouse
XT Cheng, YX Xie, B Zhou, N Huang, T Farfel-Bec, ZH Sheng Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons J. Cell Biol., 2018;0(0):. 2018 [PMID: 29695488] (Western Blot, Rat) Western Blot Rat
Show All 17 Publications.

Review for Cathepsin B Antibody (AF965) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF965:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
cynthia morimoto
IHC-P Mouse 12/19/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedKidney paraffin section
SpeciesMouse

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Bioinformatics Tool for Cathepsin B Antibody (AF965)

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Blogs on Cathepsin B.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Recent Reviews

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cynthia morimoto
12/19/2014
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CTSB
Uniprot