Cathepsin D Antibody [Unconjugated]

Images

 
Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated ...read more
Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was ...read more
Cathepsin D was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5 µg/mL for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Cathepsin D Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Ile21-Leu410
Accession # Q3UCD9
Specificity
Detects mouse Cathepsin D in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSD
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1029 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody [Unconjugated]

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC 3.4.23.5
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10

Background

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1-20), a propeptide (residues 21-64), and a mature chain (residues 65-410) (1-3). It is expressed in most cells and over-expressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

  1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
  2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
  3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
  4. Conner, (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (AF1029)(26)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(4)
IHC
(5)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(15)
All Applications
Filter By Species
Human
(1)
Mouse
(25)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF1029 Applications Species
E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022;13(1):4848. 2022 [PMID: 35977928] (Western Blot, Mouse) Western Blot Mouse
MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis The Journal of Biological Chemistry, 2022;0(0):102348. 2022 [PMID: 35933009] (Western Blot, Mouse) Western Blot Mouse
H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022;0(0):. 2022 [PMID: 35927581] (IHC, Mouse) IHC Mouse
F Barutta, S Bellini, S Kimura, K Hase, B Corbetta, A Corbelli, F Fiordaliso, S Bruno, L Biancone, A Barreca, MG Papotti, E Hirsh, M Martini, R Gambino, M Durazzo, H Ohno, G Gruden Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy Autophagy, 2022;0(0):1-20. 2022 [PMID: 35659195] (ICC, Mouse) ICC Mouse
A Skopál, T Kéki, PÁ Tóth, B Csóka, B Koscsó, ZH Német, L Antonioli, A Ivessa, F Ciruela, L Virág, G Haskó, E Kókai Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling The Journal of Biological Chemistry, 2022;0(0):101888. 2022 [PMID: 35367412] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35245440] (Western Blot, Mouse) Western Blot Mouse
K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation Journal of Neuroinflammation, 2021;18(1):164. 2021 [PMID: 34304733] (Western Blot, Mouse) Western Blot Mouse
SE Davis, JR Roth, Q Aljabi, AR Hakim, KE Savell, JJ Day, AE Arrant Delivering progranulin to neuronal lysosomes protects against excitotoxicity The Journal of Biological Chemistry, 2021;0(0):100993. 2021 [PMID: 34298019] (ICC, Mouse) ICC Mouse
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021;9(1):57. 2021 [PMID: 33795008] (Western Blot, Mouse) Western Blot Mouse
JH Lee, JY Kim, S Noh, H Lee, SY Lee, JY Mun, H Park, WS Chung Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis Nature, 2020;0(0):. 2020 [PMID: 33361813] (IHC, Mouse) IHC Mouse
Show All 26 Publications.

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Bioinformatics

Gene Symbol CTSD
Uniprot