Cathepsin D Antibody [Unconjugated]

Images

 
Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated ...read more
Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Cathepsin D Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Ile21-Leu410
Accession # Q3UCD9
Specificity
Detects mouse Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Cathepsin D is observed and less than 1% cross-reactivity with rhCathepsin A, B, C, L, X, and recombinant mouse (rm) Cathepsin B, C, H, and X is observed. In Western blots, approximately 100% cross-reactivity with rhCathepsin D is observed and less than 1% cross‑reactivity with rmCathepsin E is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSD
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF1029 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody [Unconjugated]

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC 3.4.23.5
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10

Background

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1-20), a propeptide (residues 21-64), and a mature chain (residues 65-410) (1-3). It is expressed in most cells and over-expressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

  1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
  2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
  3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
  4. Conner, (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (AF1029)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC Not Specified, WB.


Filter By Application
ICC
(1)
IHC
(1)
IHC Not Specified
(1)
WB
(7)
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Human
(1)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF1029 Applications Species
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention Sci Rep, 2016;6(0):38278. 2016 [PMID: 27922112] (IHC,, Human) IHC Human
Kenneth M Murphy RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells J. Exp. Med., 2016;0(0):. 2016 [PMID: 27899443] (WB,, Mouse) WB Mouse
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy Sci Adv, 2016;2(9):e1601167. 2016 [PMID: 27617292] (WB,, Mouse) WB Mouse
Jensen, Thomas B, Cheema, Muhammad, Szymiczek, Agata, Damkier, Helle Ha, Praetorius, Jeppe Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance. PLoS ONE, 2015;10(4):e0124902. 2015 [PMID: 25874770] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells. Mol Cancer, 2015;14(0):39. 2015 [PMID: 25744631] (WB,, Mouse) WB Mouse
Lee J, Jin H, Park M, Kim B, Lee P, Nakauchi H, Carter J, He X, Schuchman E, Bae J Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer's disease. J Exp Med, 2014;211(8):1551-70. 2014 [PMID: 25049335] (WB,, Mouse) WB Mouse
Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice. Lab Invest, 2012;92(11):1518-26. 2012 [PMID: 22825686] (WB,, Mouse) WB Mouse
Zeeuwen PL, van Vlijmen-Willems IM, Cheng T The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J., 2010;24(10):3744-55. 2010 [PMID: 20495178] (WB,, Mouse) WB Mouse
Schahs P, Weidinger P, Probst OC, Svoboda B, Stadlmann J, Beug H, Waerner T, Mach L Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis. Exp. Cell Res., 2008;314(16):3036-47. 2008 [PMID: 18621046] (WB,, Mouse) WB Mouse
Uppington H, Menager N, Boross P, Wood J, Sheppard M, Verbeek S, Mastroeni P Effect of immune serum and role of individual Fcgamma receptors on the intracellular distribution and survival of Salmonella enterica serovar Typhimurium in murine macrophages. Immunology, 2006;119(2):147-58. 2006 [PMID: 16836651] (ICC,, Mouse) ICC Mouse

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Bioinformatics

Gene Symbol CTSD
Entrez
Uniprot