Cathepsin D Antibody [Unconjugated]


Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated more
Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was more
Cathepsin D was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5 µg/mL for 3 more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ICC/IF

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Cathepsin D Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Accession # Q3UCD9
Detects mouse Cathepsin D in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF1029 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody [Unconjugated]

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10


Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1-20), a propeptide (residues 21-64), and a mature chain (residues 65-410) (1-3). It is expressed in most cells and over-expressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

  1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
  2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
  3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
  4. Conner, (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (AF1029)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF1029 Applications Species
K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation Journal of Neuroinflammation, 2021;18(1):164. 2021 [PMID: 34304733] (Western Blot, Mouse) Western Blot Mouse
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021;9(1):57. 2021 [PMID: 33795008] (Western Blot, Mouse) Western Blot Mouse
JH Lee, JY Kim, S Noh, H Lee, SY Lee, JY Mun, H Park, WS Chung Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis Nature, 2020;0(0):. 2020 [PMID: 33361813] (IHC, Mouse) IHC Mouse
S Ketterer, J Mitschke, A Ketscher, M Schlimpert, W Reichardt, N Baeuerle, ME Hess, P Metzger, M Boerries, C Peters, B Kammerer, T Brummer, F Steinberg, T Reinheckel Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling Nat Commun, 2020;11(1):5133. 2020 [PMID: 33046706] (Western Blot, Mouse) Western Blot Mouse
M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations Acta Neuropathol Commun, 2020;8(1):163. 2020 [PMID: 33028409] (IHC-P, Mouse) IHC-P Mouse
JG Li, J Chiu, M Ramanjulu, BE Blass, D Praticò A pharmacological chaperone improves memory by reducing A&beta and tau neuropathology in a mouse model with plaques and tangles Mol Neurodegener, 2020;15(1):1. 2020 [PMID: 31964406] (Western Blot, Mouse) Western Blot Mouse
Q Shao, M Yang, C Liang, L Ma, W Zhang, Z Jiang, J Luo, JK Lee, C Liang, JF Chen C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy, 2019;0(0):1-16. 2019 [PMID: 31847700] (ICC, Mouse) ICC Mouse
Y Atiskova, S Bartsch, T Danyukova, E Becker, C Hagel, S Storch, U Bartsch Mice deficient in the lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) display a complex retinal phenotype Sci Rep, 2019;9(1):14185. 2019 [PMID: 31578378] (Western Blot, Mouse) Western Blot Mouse
A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy Exp. Eye Res., 2018;172(0):1-9. 2018 [PMID: 29596849] (IHC, Mouse) IHC Mouse
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention Sci Rep, 2016;6(0):38278. 2016 [PMID: 27922112] (IHC, Human) IHC Human
Show All 19 Publications.

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Secondary Antibodies


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Biotin BAF1029

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Gene Symbol CTSD