Cathepsin D Antibody [Unconjugated]


Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated more
Cathepsin D was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5 µg/mL for 3 more
Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ICC/IF

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Cathepsin D Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Accession # Q3UCD9
Detects mouse Cathepsin D in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF1029 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin D Antibody [Unconjugated]

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10


Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1-20), a propeptide (residues 21-64), and a mature chain (residues 65-410) (1-3). It is expressed in most cells and over-expressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

  1. Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
  2. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. 
  3. Hetman, et al. (1994) DNA Cell Biol. 13:419.
  4. Conner, (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (AF1029)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 8 applications: Functional Assay, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

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Functional Assay
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF1029 Applications Species
SE Davis, AK Cook, JA Hall, Y Voskobiyny, NV Carullo, NR Boyle, AR Hakim, KM Anderson, KP Hobdy, DA Pugh, CF Murchison, LJ McMeekin, M Simmons, KA Margolies, RM Cowell, AL Nana, S Spina, LT Grinberg, BL Miller, WW Seeley, AE Arrant Patients with sporadic FTLD exhibit similar increases in lysosomal proteins and storage material as patients with FTD due to GRN mutations Acta neuropathologica communications, 2023-04-28;11(1):70. 2023-04-28 [PMID: 37118844] (Western Blot, Mouse) Western Blot Mouse
J Tunganunta, P Kanjanasir, T Khumpanied, S Benjaskull, B Wongprom, T Palaga, TAP Siregar, S Borwornpin, A Chaipraser, P Palittapon, M Ponpuak BORC complex specific components and Kinesin-1 mediate autophagy evasion by the autophagy-resistant Mycobacterium tuberculosis Beijing strain Scientific Reports, 2023-01-30;13(1):1663. 2023-01-30 [PMID: 36717601] (ICC/IF, Mouse) ICC/IF Mouse
YX Xie, NN Naseri, J Fels, P Kharel, Y Na, D Lane, J Burré, M Sharma Lysosomal exocytosis releases pathogenic alpha-synuclein species from neurons in synucleinopathy models Nature Communications, 2022-08-22;13(1):4918. 2022-08-22 [PMID: 35995799] (Functional Assay, Transgenic Mouse) Functional Assay Transgenic Mouse
E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly Nature Communications, 2022-08-17;13(1):4848. 2022-08-17 [PMID: 35977928] (Western Blot, Mouse) Western Blot Mouse
MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis The Journal of Biological Chemistry, 2022-08-04;0(0):102348. 2022-08-04 [PMID: 35933009] (Western Blot, Mouse) Western Blot Mouse
H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022-08-04;0(0):. 2022-08-04 [PMID: 35927581] (IHC, Mouse) IHC Mouse
F Barutta, S Bellini, S Kimura, K Hase, B Corbetta, A Corbelli, F Fiordaliso, S Bruno, L Biancone, A Barreca, MG Papotti, E Hirsh, M Martini, R Gambino, M Durazzo, H Ohno, G Gruden Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy Autophagy, 2022-06-06;0(0):1-20. 2022-06-06 [PMID: 35659195] (ICC, Mouse) ICC Mouse
A Skopál, T Kéki, PÁ Tóth, B Csóka, B Koscsó, ZH Német, L Antonioli, A Ivessa, F Ciruela, L Virág, G Haskó, E Kókai Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling The Journal of Biological Chemistry, 2022-03-31;0(0):101888. 2022-03-31 [PMID: 35367412] (ICC, Immunoprecipitation, Western Blot, Mouse) ICC, Immunoprecipitation, Western Blot Mouse
K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids Stem Cell Reports, 2022-03-03;0(0):. 2022-03-03 [PMID: 35245440] (Western Blot, Mouse) Western Blot Mouse
K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation Journal of Neuroinflammation, 2021-07-25;18(1):164. 2021-07-25 [PMID: 34304733] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Show All 29 Publications.

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Gene Symbol CTSD