DC-LAMP Antibody (1010E1.01)

Immunohistochemistry: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - TLSs are highly organized structures in OSCC tissues and appear as clusters of B-cell follicles surrounded by T-cell zones and HEVs. c Representative multiplex IHC images showing immune cells of immature TLS and mature TLS samples by simultaneous staining of B cells (CD20, yellow), T cells (CD3, red), mature dendritic cells (DC-LAMP, green), high endothelial venules (PNAd, cyan) and the nuclear stain DAPI (blue) (x200, original magnification). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32934197) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Paraffin embedded lung tumor section stained with DC-LAMP

Immunohistochemistry-Paraffin: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Staining of sentinel lymph nodes from 458 patients with DC-LAMP

Immunohistochemistry-Paraffin: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Paraffin staining of canine issues

Immunohistochemistry: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - Analysis of human lymph node paraffin section with clone 1010E1 (DX0191)

Immunohistochemistry: DC-LAMP Antibody (1010E1.01) [DDX0191P-100] - IHC on mouse lungcryosection

• Reactivity: Hu, Mu, Ca, Fe, Sh
• Applications: ICC/IF, IHC
• Clone: 1010E1.01
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DC-LAMP Antibody (1010E1.01) Summary

• Immunogen: DC-LAMP/CD208 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LAMP3
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Camelids, ferret (Mustela putorius furo) Mouse reactivity reported in scientific literature (PMID:32989251).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for DC-LAMP Antibody (1010E1.01)

• CD208 antigen
• CD208
• DCLAMP
• DC-LAMP
• DCLAMPDC-lysosome-associated membrane glycoprotein
• LAMP
• LAMP3
• LAMP-3
• lysosomal-associated membrane protein 3DC LAMP
• lysosome-associated membrane glycoprotein 3
• Protein TSC403
• TSC403
• TSC403CD208

Background

Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DC-LAMP Antibody (DDX0191P-100)(46)

Publications using DDX0191P-100

• Callaway DA, Penkala IJ, Zhou S et al. TGF? controls alveolar type 1 epithelial cell plasticity and alveolar matrisome gene transcription bioRxiv : the preprint server for biology 2023-05-10 [PMID: 37214932]
• Cardenas-Diaz FL, Liberti DC, Leach JP et al. Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1 Cell reports 2023-04-27 [PMID: 37119134]
• Shiraishi K, Shah PP, Morley MP et al. Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate Cell 2023-02-23 [PMID: 36870331] (IHC-P, Mouse)
• Tang AT, Buchholz DW, Szigety KM et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PLoS biology 2023-02-01 [PMID: 36745682] (IHC, Mouse)
• Kato T, Asakura T, Edwards CE et al. Prevalence and Mechanisms of Mucus Accumulation in COVID-19 Lung Disease American journal of respiratory and critical care medicine 2022-07-11 [PMID: 35816430]
• Leader A, Grout J, Chang C et al. CITEseq analysis of non-small-cell lung cancer lesions reveals an axis of immune cell activation associated with tumor antigen load and TP53 mutations bioRxiv 2020-07-17
• Wu L, Sun Y, Zhong Y et al. Elaborating the Tumor Ecosystem of Primary and Relapsed Hepatocellular Carcinoma by Single-Cell RNA Sequencing SSRN Journal 2019-10-26
• Liberti DC, Liberti Iii WA, Kremp MM et al. Klf5 defines alveolar epithelial type 1 cell lineage commitment during lung development and regeneration Developmental cell 2022-07-25 [PMID: 35803279]
• Johnson BE, Creason AL, Stommel JM et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer Cell reports. Medicine 2022-02-15 [PMID: 35243422]
• Sawada J, Hiraoka N, Qi R et al. Molecular Signature of Tumor-Associated High Endothelial Venules That Can Predict Breast Cancer Survival Cancer immunology research 2022-04-01 [PMID: 35201289] (IHC-P, Human)
  
  Details:
  Human tonsils
• Shyam R, Ogando DG, Choi M et al. Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial Dystrophy Investigative ophthalmology & visual science 2021-09-02 [PMID: 34533563] (IF/IHC, Mouse)
• Cheng S, Li Z, Gao R et al. A pan-cancer single-cell transcriptional atlas of tumor infiltrating myeloid cells Cell 2021-02-04 [PMID: 33545035] (IHC-P, Human)
  
  Details:
  Multi-color immuonhistochemistry
• Katsura H, Sontake V, Tata A et al. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction Cell Stem Cell 2020-10-01 [PMID: 33128895] (IHC-P, Human)
• Leist SR, Dinnon KH, SchAfer A et al. A Mouse-Adapted SARS-CoV-2 Induces Acute Lung Injury and Mortality in Standard Laboratory Mice Cell 2020-09-23 [PMID: 33031744] (IF/IHC, Mouse)
• Paris AJ, Hayer KE, Oved JH et al. Increased Transient Receptor Potential Melastatin 8 Expression in the development of bladder pain in patients with Interstitial cystitis/Bladder Pain Syndrome Nat Cell Biol 2020-10-10 [PMID: 32989251] (IHC-P, Mouse)
• Li Q, Liu X, Wang D et al. Prognostic value of tertiary lymphoid structure and tumour infiltrating lymphocytes in oral squamous cell carcinoma Int J Oral Sci 2020-09-15 [PMID: 32934197] (IF/IHC, Human)
• Derks S, de Klerk LK, Xu X et al. Characterizing diversity in the tumor-immune microenvironment of distinct subclasses of gastroesophageal adenocarcinomas Ann. Oncol. 2020-05-06 [PMID: 32387455] (IF/IHC)
• Daniel E, Barlow HR, Sutton GI et al. Cyp26b1 is a critical regulator of distal airway epithelial differentiation during lung development Development 2020-01-30 [PMID: 32001436] (ICC/IF)
• Restrepo P, Yong R, Laface I et al. Tumoral and immune heterogeneity in an anti-PD-1 responsive glioblastoma: a case study Cold Spring Harb Mol Case Stud 2020-01-06 [PMID: 31907277] (IF/IHC, Human)
• Weiner AI, Jackson SR, Zhao G et al. Mesenchyme-free expansion and transplantation of adult alveolar progenitor cells: steps toward cell-based regenerative therapies NPJ Regen Med 2019-08-20 [PMID: 31452939] (ICC/IF, Mouse)
• Koti M, Xu AS, Ren kYM et al. Tertiary Lymphoid Structures Associate with Tumour Stage in Urothelial Bladder Cancer. Bladder Cancer. [PMID: 29152550] (IF/IHC, Human)
• Balachandran VP, tuksza M, Zhao JN et al. Identification of unique neoantigen qualities in long-term survivors of pancreatic cancer Nature. 2017-11-23 [PMID: 29132146] (Human)
• Garcia-Hernandez ML, Uribe-Uribe NO, Espinosa-Gonzalez R et al. A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression.. Front Immunol 2017-06-01 [PMID: 28567040] (ICC/IF, Human)
• Lavin Y, Kobayashi S et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell 2017-04-05 [PMID: 28475900] (IF/IHC, Human)
• Hervier B et al. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome. J Immunol 2016-09-01 [PMID: 27511738]
• Thongtharb A et al. Histological and immunohistochemical studies on primary intracranial canine histiocytic sarcomas. J Vet Med Sci 2016-05-01 [PMID: 26668164]
• Monteiro-Sepulveda M et al. Jejunal T Cell Inflammation in Human Obesity Correlates with Decreased Enterocyte Insulin Signaling. Cell Metab 2015-07-01 [PMID: 26094890]
• Cousens C et al. Jaagsiekte sheep retrovirus infection of lung slice cultures. Retrovirology 2015-04-01 [PMID: 25889156]
• Theate I et al. Extensive profiling of the expression of the indoleamine 2,3-dioxygenase 1 protein in normal and tumoral human tissues. Cancer Immunol Res 2015-02-01 [PMID: 25271151]
• Alifano M et al. Systemic inflammation, nutritional status and tumor immune microenvironment determine outcome of resected non-small cell lung cancer. PLoS One 2014-09-01 [PMID: 25238252]
• Germain C et al. Presence of B cells in tertiary lymphoid structures is associated with a protective immunity in patients with lung cancer. Am J Respir Crit Care Med 2014-04-01 [PMID: 24484236]
• Goc J et al. Dendritic cells in tumor-associated tertiary lymphoid structures signal a Th1 cytotoxic immune contexture and license the positive prognostic value of infiltrating CD8+ T cells. Cancer Res 2014-02-01 [PMID: 24366885]
• Chang DR et al. Lung epithelial branching program antagonizes alveolar differentiation. Proc Natl Acad Sci U S A 2013-11-01 [PMID: 24058167]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Martinet L et al. High endothelial venule blood vessels for tumor-infiltrating lymphocytes are associated with lymphotoxin beta-producing dendritic cells in human breast cancer. J Immunol 2013-08-01 [PMID: 23825314]
• Remark R et al. Characteristics and clinical impacts of the immune environments in colorectal and renal cell carcinoma lung metastases: influence of tumor origin. Clin Cancer Res 2013-08-01 [PMID: 23785047]
• Berbic M et al. A novel pilot study of endometrial stromal cells and immune cell populations in sentinel uterine-draining lymph nodes during the menstrual cycle and in endometriosis. Reprod Sci 2013-11-01 [PMID: 23719712]
• Romero-Palomo F et al. Immunohistochemical detection of dendritic cell markers in cattle. Vet Pathol 2013-11-01 [PMID: 23528943]
• Bailey C et al. Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog 2012-09-01 [PMID: 22969429]
• Dieu-Nosjean MC et al. Long-term survival for patients with non-small-cell lung cancer with intratumoral lymphoid structures. J Clin Oncol 2008-09-01 [PMID: 18802153]
• Elliot B et al. Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma. Clin Cancer Res 2007-07-01 [PMID: 17606713]
• Treilleux I et al. Dendritic cell infiltration and prognosis of early stage breast cancer. Clin Cancer Res 2004-11-01 [PMID: 15569976]
• Movassagh M et al. Selective accumulation of mature DC-Lamp+ dendritic cells in tumor sites is associated with efficient T-cell-mediated antitumor response and control of metastatic dissemination in melanoma. Cancer Res 2004-03-15 [PMID: 15026362]
• Salaun B et al. CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol 2004-03-01 [PMID: 14982840]
• Salaun B et al. Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol 2003-09-01 [PMID: 12938238]
• de Saint Vis B et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity 1998-09-01 [PMID: 9768752]

Review for DC-LAMP Antibody (DDX0191P-100) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 09/25/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil tissue
• Species: Human
• Lot: PDX0191-048

Bioinformatics

• Gene Symbol: LAMP3