Rab7a Antibody

Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rab7a Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAK
• Marker: Late Endosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Rab7a Protein (NBP1-87174PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rab7a Antibody

• CMT2B
• FLJ20819
• member RAS oncogene family
• PRO2706
• PSN
• RAB7
• Rab7a
• RAB7A, member RAS oncogene family
• Ras-associated protein RAB7
• ras-related protein Rab-7a

Background

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab7a Antibody (NBP1-87174)(2)

Publications using NBP1-87174

• Pera M, Larrea D, Guardia-Laguarta C et al. Increased localization of APP-C99 in mitochondria-associated ER membranes causes mitochondrial dysfunction in Alzheimer disease. EMBO J. 2017-10-10 [PMID: 29018038] (WB, Mouse)
• Peinado JR, Chaplot K, Jarvela TS, Barbieri E Phase separation of proSAAS into spheres results in core sequestration of TDP-43216-414 aggregates bioRxiv 2020-01-01

Review for Rab7a Antibody (NBP1-87174) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: NIKHIL VAD IF Human 07/10/2018

Summary

• Application: Immunofluorescence
• Sample Tested: A172 Glioblastoma cells
• Species: Human
• Lot: C114852

Comments

• Comments: Cell were grown on coverslips overnight, media was aspirated and cells fixed with 4% paraformaldehyde for 20 min. Following 3x washes with PBS, cells were permeabilized with ice-cold methanol for 10 min. Following 3x washes, cells were blocked overnight with blocking buffer (0.3% triton-X 100, 5% serum in PBS). The following day, cells were incubated with primary Rab7a antibody (1:200) for 1 h at RT. Following 3x washes in PBS, cells were incubated with anti-rabbit AF488 secondary antibody for 1 h at RT. Following incubation, cells were washes 3x with PBS, mounted on slides using Prolong Gold (with DAPI) and imaged using a Nikon Confocal microscope.

Bioinformatics

• Gene Symbol: RAB7A
• Entrez:
  • 7879(Human)
  • 19349(Mouse)
• Uniprot:
  • P51149(Human)