Genetic Strategies: Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
This antibody was developed against Recombinant Protein corresponding to amino acids: CVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAK
Late Endosome Marker
Specificity of human Rab7a antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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WB reported in scientific literature (PMID: 29018038). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. ICC/IF customer review.
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP1-87174 in the following applications:
Cell were grown on coverslips overnight, media was aspirated and cells fixed with 4% paraformaldehyde for 20 min. Following 3x washes with PBS, cells were permeabilized with ice-cold methanol for 10 min. Following 3x washes, cells were blocked overnight with blocking buffer (0.3% triton-X 100, 5% serum in PBS). The following day, cells were incubated with primary Rab7a antibody (1:200) for 1 h at RT. Following 3x washes in PBS, cells were incubated with anti-rabbit AF488 secondary antibody for 1 h at RT. Following incubation, cells were washes 3x with PBS, mounted on slides using Prolong Gold (with DAPI) and imaged using a Nikon Confocal microscope.
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