Genetic Strategies: Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
This antibody was developed against Recombinant Protein corresponding to amino acids: CVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAK
Late Endosome Marker
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Rab7a Antibody
member RAS oncogene family
RAB7A, member RAS oncogene family
Ras-associated protein RAB7
ras-related protein Rab-7a
RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Cell were grown on coverslips overnight, media was aspirated and cells fixed with 4% paraformaldehyde for 20 min. Following 3x washes with PBS, cells were permeabilized with ice-cold methanol for 10 min. Following 3x washes, cells were blocked overnight with blocking buffer (0.3% triton-X 100, 5% serum in PBS). The following day, cells were incubated with primary Rab7a antibody (1:200) for 1 h at RT. Following 3x washes in PBS, cells were incubated with anti-rabbit AF488 secondary antibody for 1 h at RT. Following incubation, cells were washes 3x with PBS, mounted on slides using Prolong Gold (with DAPI) and imaged using a Nikon Confocal microscope.
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