Rab7a Antibody

Images

 
Genetic Strategies: Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Rab7a Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAK
Marker
Late Endosome Marker
Specificity
Specificity of human Rab7a antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB7A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
Application Notes
WB reported in scientific literature (PMID: 29018038). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. ICC/IF customer review.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Reviewed Applications
Read 1 Review rated 4
using
NBP1-87174 in the following applications:

Publications
Read Publication using
NBP1-87174 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rab7a Antibody

  • CMT2B
  • FLJ20819
  • member RAS oncogene family
  • PRO2706
  • PSN
  • RAB7
  • Rab7a
  • RAB7A, member RAS oncogene family
  • Ras-associated protein RAB7
  • ras-related protein Rab-7a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-87174
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for Rab7a Antibody (NBP1-87174)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Rab7a Antibody (NBP1-87174) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-87174:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Rab7a NBP1-87174
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reviewed by:
Anonymous
IF Human 07/10/2018
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Summary

ApplicationImmunofluorescence
Sample TestedA172 Glioblastoma cells
SpeciesHuman
LotC114852

Comments

CommentsCell were grown on coverslips overnight, media was aspirated and cells fixed with 4% paraformaldehyde for 20 min. Following 3x washes with PBS, cells were permeabilized with ice-cold methanol for 10 min. Following 3x washes, cells were blocked overnight with blocking buffer (0.3% triton-X 100, 5% serum in PBS). The following day, cells were incubated with primary Rab7a antibody (1:200) for 1 h at RT. Following 3x washes in PBS, cells were incubated with anti-rabbit AF488 secondary antibody for 1 h at RT. Following incubation, cells were washes 3x with PBS, mounted on slides using Prolong Gold (with DAPI) and imaged using a Nikon Confocal microscope.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab7a Antibody (NBP1-87174) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
07/10/2018
Application: IF
Species: Human

Bioinformatics

Gene Symbol RAB7A
Entrez
Uniprot