EEA1 Antibody

Images

 
Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug HepG2 whole cell lysate/extract B. 30 ug A375 whole cell lysate/extract 5 % SDS-PAGE EEA1 antibody [C3], C-termdilution: 1:5000
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody [NBP1-30914] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 protein stained by EEA1 antibody [C3], C-term ...read more
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma; antibody dilution 1:500.
Western Blot: EEA1 Antibody [NBP1-30914] - A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (was used to detect the primary antibody.
Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa, using EEA1 (NBP1-30914) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse hind brain.
Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis.

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EEA1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.
Localization
Cytoplasm, Early endosome membrane
Marker
Early Endosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-30914 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEA1 Antibody

  • early endosome antigen 1
  • early endosome antigen 1, 162kD
  • early endosome-associated protein
  • EEA1
  • Endosome-associated protein p162
  • MST105
  • MSTP105
  • ZFYVE2
  • ZFYVE2MST105
  • Zinc finger FYVE domain-containing protein 2

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-30914
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Publications for EEA1 Antibody (NBP1-30914)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IM.


Filter By Application
ICC/IF
(3)
IM
(1)
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Filter By Species
Human
(3)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional EEA1 Products

Bioinformatics Tool for EEA1 Antibody (NBP1-30914)

Discover related pathways, diseases and genes to EEA1 Antibody (NBP1-30914). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EEA1 Antibody (NBP1-30914)

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Research Areas for EEA1 Antibody (NBP1-30914)

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Blogs on EEA1.

Understanding EEA1's Role in Membrane Endosome Fusion
EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo...  Read full blog post.

EEA1 Antibodies in Endosomal Transport and Signalling Studies
EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have...  Read full blog post.

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Bioinformatics

Gene Symbol EEA1
Entrez
Uniprot