LAMP-2/CD107b Antibody (H4B4) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, Ch, Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF, KO
Clone
H4B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

LAMP-2/CD107b Antibody (H4B4) [Janelia Fluor® 646] Summary

Immunogen
LAMP-2/CD107b Antibody (H4B4) was made using a human adherent spleen cells.
Localization
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Marker
Late Endosome / Lysosome marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAMP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
  • Knockout Validated
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27863209). Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for LAMP-2/CD107b Antibody (H4B4) [Janelia Fluor® 646]

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMPB
  • LGP110
  • LGP-96
  • Lysosomal Associated Membrane Protein 2
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). LAMP-2 protein is encoded by nine exons, with the first 8 exons and a portion of exon 9 encoding the highly glycosylated protein domains within the lysosomal lumen. The transmembrane and cytosolic carboxy-terminal domains of LAMP-2 are encoded by the remaining sequence of exon 9 and conform the receptor for targeting proteins to the lysosome. Splicing of exon 9 in the LAMP-2 pre mRNA leads to various splice forms with distinct cytosolic domains. Three splice variants, LAMP-2A, -2B and -2C, have been identified which shuttle between the plasma membrane, endosomal compartment and lysosomes (1). Tissue specific expression has been described for each LAMP-2 splice variant, with LAMP-2A being more ubiquitously expressed (e.g., placenta, lung, liver, pancreas and prostate), LAMP-2B predominantly expressed in skeletal muscle and LAMP-2C in brain tissue (1). All LAMP-2 splice variants participate in lysosomal degradation processes. LAMP-2A is the only variant that serves as a receptor targeting proteins for lysosomal degradation in chaperone-mediated autophagy (2,3). LAMP-2B is essential for macroautophagy in cardiomyocytes, where it facilitates autophagosome-lysosome fusion. LAMP-2B mutations underscore the myopathy and severe hypertrophic cardiomyopathy in Danon disease which results from deficits in autophagy (1, 4). Vasculopathy of coronary and cerebral arteries is a rare phenotype in Danon patients that is also associated with deficient autophagy processing of proteins and cellular organelles (5). LAMP2C serves as a receptor for DNA and RNA, facilitating their lysosomal degradation through DNA-autophagy and RNA-autophagy, respectively (1).

References

1. Rowland, T. J., Sweet, M. E., Mestroni, L., & Taylor, M. R. G. (2016). Danon disease - dysregulation of autophagy in a multisystem disorder with cardiomyopathy. Journal of Cell Science. https://doi.org/10.1242/jcs.184770

2. Alfaro, I. E., Albornoz, A., Molina, A., Moreno, J., Cordero, K., Criollo, A., & Budini, M. (2019). Chaperone mediated autophagy in the crosstalk of neurodegenerative diseases and metabolic disorders. Frontiers in Endocrinology. https://doi.org/10.3389/fendo.2018.00778

3. Schneider, J. L., & Cuervo, A. M. (2014). Autophagy and human disease: Emerging themes. Current Opinion in Genetics and Development. https://doi.org/10.1016/j.gde.2014.04.003

4. Chi, C., Leonard, A., Knight, W. E., Beussman, K. M., Zhao, Y., Cao, Y., Song, K. (2019). LAMP-2B regulates human cardiomyocyte function by mediating autophagosome lysosome fusion. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1808618116

5. Nguyen, H. T., Noguchi, S., Sugie, K., Matsuo, Y., Nguyen, C. T. H., Koito, H., Tsukaguchi, H. (2018). Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Scientific Reports. https://doi.org/10.1038/s41598-018-21602-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-22217JF646) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22217AF350
Alexa Fluor 405 NBP2-22217AF405
Alexa Fluor 488 NBP2-22217AF488
Alexa Fluor 532 NBP2-22217AF532
Alexa Fluor 594 NBP2-22217AF594
Alexa Fluor 647 NBP2-22217AF647
Alexa Fluor 700 NBP2-22217AF700
Alexa Fluor 750 NBP2-22217AF750
Allophycocyanin NBP2-21799
Allophycocyanin/Cy7 NBP2-22217APCCY7
Biotin NBP2-22217B
DyLight 350 NBP2-22217UV
DyLight 405 NBP2-22217V
DyLight 488 NBP2-22217G
DyLight 550 NBP2-22217R
DyLight 594 NBP2-22217DL594
DyLight 650 NBP2-22217C
DyLight 680 NBP2-22217FR
DyLight 755 NBP2-22217IR
FITC NBP1-28553
HRP NBP2-22217H
Janelia Fluor 549 NBP2-22217JF549
Janelia Fluor 646 NBP2-22217JF646
PE NBP2-22217PE
PE/Atto594 NBP2-22217PEATT594
PE/Cy5.5 NBP2-22217PECY55
PE/Cy7 NBP2-22217PECY7
PerCP NBP2-22217PCP

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Chaperone Mediated Autophagy (CMA) does it all!
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Bioinformatics

Gene Symbol LAMP2
Uniprot