LAMP-2/CD107b Antibody (H4B4)

Images

 
Genetic Strategies: Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Immunofluorescence: [Alexa Fluor® 647] [NBP2-22217AF647] - T98G glioblastoma cells grown on #1.5 coverglass stained with a ...read more
Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.
Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on Jurkat cells with LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of anti-LAMP-2/CD107b. Staining of 5 ul overlay with isotype on U937 Cells.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Using the Alexa Fluor 647 direct conjugate, an intracellular stain was performed on HeLa cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on SK-MEL-28 cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were ...read more
ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted ...read more

Product Details

Summary
Reactivity Hu, Mu, Ch, Rt(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
Clone
H4B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

LAMP-2/CD107b Antibody (H4B4) Summary

Immunogen
LAMP-2/CD107b Antibody (H4B4) was made using a human adherent spleen cells.
Localization
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Marker
Late Endosome / Lysosome marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAMP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • CyTOF-ready
  • Knockout Validated
Application Notes
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Bladder Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-22217 in the following applications:

Publications
Read Publications using
NBP2-22217 in the following applications:

  • IB
    1 publication
  • 9 publications
  • IF
    2 publications
  • IHC
    2 publications
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27863209). Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LAMP-2/CD107b Antibody (H4B4)

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMPB
  • LGP110
  • LGP-96
  • Lysosomal Associated Membrane Protein 2
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). LAMP-2 protein is encoded by nine exons, with the first 8 exons and a portion of exon 9 encoding the highly glycosylated protein domains within the lysosomal lumen. The transmembrane and cytosolic carboxy-terminal domains of LAMP-2 are encoded by the remaining sequence of exon 9 and conform the receptor for targeting proteins to the lysosome. Splicing of exon 9 in the LAMP-2 pre mRNA leads to various splice forms with distinct cytosolic domains. Three splice variants, LAMP-2A, -2B and -2C, have been identified which shuttle between the plasma membrane, endosomal compartment and lysosomes (1). Tissue specific expression has been described for each LAMP-2 splice variant, with LAMP-2A being more ubiquitously expressed (e.g., placenta, lung, liver, pancreas and prostate), LAMP-2B predominantly expressed in skeletal muscle and LAMP-2C in brain tissue (1). All LAMP-2 splice variants participate in lysosomal degradation processes. LAMP-2A is the only variant that serves as a receptor targeting proteins for lysosomal degradation in chaperone-mediated autophagy (2,3). LAMP-2B is essential for macroautophagy in cardiomyocytes, where it facilitates autophagosome-lysosome fusion. LAMP-2B mutations underscore the myopathy and severe hypertrophic cardiomyopathy in Danon disease which results from deficits in autophagy (1, 4). Vasculopathy of coronary and cerebral arteries is a rare phenotype in Danon patients that is also associated with deficient autophagy processing of proteins and cellular organelles (5). LAMP2C serves as a receptor for DNA and RNA, facilitating their lysosomal degradation through DNA-autophagy and RNA-autophagy, respectively (1).

References

1. Rowland, T. J., Sweet, M. E., Mestroni, L., & Taylor, M. R. G. (2016). Danon disease - dysregulation of autophagy in a multisystem disorder with cardiomyopathy. Journal of Cell Science. https://doi.org/10.1242/jcs.184770

2. Alfaro, I. E., Albornoz, A., Molina, A., Moreno, J., Cordero, K., Criollo, A., & Budini, M. (2019). Chaperone mediated autophagy in the crosstalk of neurodegenerative diseases and metabolic disorders. Frontiers in Endocrinology. https://doi.org/10.3389/fendo.2018.00778

3. Schneider, J. L., & Cuervo, A. M. (2014). Autophagy and human disease: Emerging themes. Current Opinion in Genetics and Development. https://doi.org/10.1016/j.gde.2014.04.003

4. Chi, C., Leonard, A., Knight, W. E., Beussman, K. M., Zhao, Y., Cao, Y., Song, K. (2019). LAMP-2B regulates human cardiomyocyte function by mediating autophagosome lysosome fusion. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1808618116

5. Nguyen, H. T., Noguchi, S., Sugie, K., Matsuo, Y., Nguyen, C. T. H., Koito, H., Tsukaguchi, H. (2018). Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Scientific Reports. https://doi.org/10.1038/s41598-018-21602-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-2/CD107b Antibody (NBP2-22217)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: IB, ICC/IF, IF, IHC, WB.


Filter By Application
IB
(1)
ICC/IF
(9)
IF
(2)
IHC
(2)
WB
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(3)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NBP2-22217 Applications Species
Ahmed M, Baumgartner R, Aldi S et al. Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors Int J Nanomedicine 2019 May 21 [PMID: 31190821] (IF, Human) IF Human
Chang-Graham AL, Danhof HA, Engevik MA et al. Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions Mol. Cell. Biol. May 6 2019 [PMID: 31061094]
Wong M, Ganapathy AS, Suchanec E et al. Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1 Am. J. Physiol., Cell Physiol. Mar 20 2019 [PMID: 30892937] (ICC/IF, Human) ICC/IF Human
Masso-Welch P, Girald Berlingeri S, King-Lyons ND et al. LT-IIc, A Bacterial Type II Heat-Labile Enterotoxin, Induces Specific Lethality in Triple Negative Breast Cancer Cells by Modulation of Autophagy and Induction of Apoptosis and Necroptosis. Int J Mol Sci Dec 26 2018 [PMID: 30587795] (ICC/IF, Human) ICC/IF Human
Jones JR, kong L, Hanna MG et al. Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes. Cell Rep. Oct 23 2018 [PMID: 30355500] (ICC/IF, Mouse) ICC/IF Mouse
Furuya N, kakuta S, Sumiyoshi k et al. NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy. EMBO Rep. Oct 11 2018 [PMID: 30309841] (Human) Human
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. Sep 21 2018 [PMID: 30298003] (WB, Chicken) WB Chicken
Swystun LL, Lai JD, Notley C et al. The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity J. Clin. Invest. Aug 31 2018 [PMID: 30124466] (ICC/IF, Human) ICC/IF Human
Pinazza Marica, Ghisi Margherita, Minuzzo Sonia et al. Histone deacetylase 6 controls Notch3 trafficking and degradation in T-cell acute lymphoblastic leukemia cells. Oncogene 2018 Apr 12 [PMID: 29643474] (IB, IF, Mouse, Human) IB, IF Mouse, Human
Nguyen HT, Noguchi S, Sugie K, Matsuo Y. Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Sci Rep. 2018 Feb 20 [PMID: 29463847] (ICC/IF, Human) ICC/IF Human
Show All 20 Publications.

Reviews for LAMP-2/CD107b Antibody (NBP2-22217) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-22217:
Filter by Applications
ELISA
(1)
WB
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
ELISA LAMP-2/CD107b NBP2-22217
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reviewed by:
Anonymous
ELISA Human 06/05/2018
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Summary

ApplicationELISA
Sample TestedSVG-A cells
SpeciesHuman
LotA-6
 
reviewed by:
Anonymous
WB Human 06/03/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage whole cell lysate
SpeciesHuman
LotA-3

Blocking

Blocking Details5% Skimmed milk in 0.1% Tween20 1XPBS, 1h, RT

Primary Anitbody

Dilution Ratio1:5000 in 5% Skimmed milk in 0.1% Tween20 1XPBS, overnight at 4 degree celcius

Secondary Antibody

Secondary Manufacturer Cat#WB7104
Secondary ConcentrationReady to use solution for secondary antibody

Details

Detection NotesWesternBreeze Chemiluminiscent Detection kit using automated X Ray developing system exposure time is 30 seconds to 1 min

Comments

CommentsThis antibody is very sensitive so final dilution can be higher than 5000 in cell type dependent manner and exposure time during detection has to be very low.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-22217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22217AF350
Alexa Fluor 405 NBP2-22217AF405
Alexa Fluor 488 NBP2-22217AF488
Alexa Fluor 532 NBP2-22217AF532
Alexa Fluor 594 NBP2-22217AF594
Alexa Fluor 647 NBP2-22217AF647
Alexa Fluor 700 NBP2-22217AF700
Alexa Fluor 750 NBP2-22217AF750
Allophycocyanin NBP2-21799
Allophycocyanin/Cy7 NBP2-22217APCCY7
Biotin NBP2-22217B
DyLight 350 NBP2-22217UV
DyLight 405 NBP2-22217V
DyLight 488 NBP2-22217G
DyLight 550 NBP2-22217R
DyLight 594 NBP2-22217DL594
DyLight 650 NBP2-22217C
DyLight 680 NBP2-22217FR
DyLight 755 NBP2-22217IR
FITC NBP1-28553
HRP NBP2-22217H
Janelia Fluor 549 NBP2-22217JF549
Janelia Fluor 646 NBP2-22217JF646
PE NBP2-22217PE
PE/Atto594 NBP2-22217PEATT594
PE/Cy5.5 NBP2-22217PECY55
PE/Cy7 NBP2-22217PECY7
PerCP NBP2-22217PCP

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Bioinformatics Tool for LAMP-2/CD107b Antibody (NBP2-22217)

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Blogs on LAMP-2/CD107b.

Chaperone Mediated Autophagy (CMA) does it all!
By Christina Towers, PhD. The degradation of cellular proteins is a critical step of both regulation and quality control and results in the turn over and recycling of critical amino acids. The two main mechanisms o...  Read full blog post.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

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Recent Reviews

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Anonymous
06/05/2018
Application: ELISA
Species: Human

 
Anonymous
06/03/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol LAMP2
Uniprot