Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of NBP2-22217. Staining of 5ul overlay with isotype on U937 Cells.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration ...read more
Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Simple Western lane view shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Late Endosome / Lysosome marker
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Western Blot 1:1000
Simple Western 1:25
Flow Cytometry 1 ug per million cells
Immunohistochemistry 1:100 - 1:200
Immunohistochemistry-Frozen 1:100 - 1:200
Immunohistochemistry-Paraffin 1:100 - 1:200
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read 1 Review rated 5 using NBP2-22217 in the following applications:
LAMP2 (Lysosome-associate protein 2) is one of a family of membrane glycoproteins whose function is to provide selectins with carbohydrate ligands. LAMP2 has been implicated in tumor metastasis, as well as overall protection, maintenance and adhesion of the lysosome. Specifically, LAMP2 may protect the lysosomal membrane from auto digestion, and also maintain the acidic environment necessary for the lysosome to function correctly. On a subcellular level, LAMP2 is expressed as a single-pass type I membrane protein, shuttling between endosomes, lysosomes, and the plasma membrane. Mutations in LAMP2 lead to a lysosomal glycogen storage disease known as "Danon Disease". Recently, caspase activation has been suggested in the extracellular export of autophagic vesicles, such as LAMP2 (PMID: 22692030).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.