LAMP-2/CD107b Antibody (H4B4)

Images

 
Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with LAMP-2/CD107b (H4B4) NBP2-22217AF647 (blue) and a matched ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration ...read more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of NBP2-22217. Staining of 5ul overlay with isotype on U937 Cells.
Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Simple Western lane view shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
H4B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

LAMP-2/CD107b Antibody (H4B4) Summary

Immunogen
Human adherent spleen cells.
Localization
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Marker
Late Endosome / Lysosome marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAMP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • CyTOF-ready
Application Notes
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Bladder Lysate (NB820-60568)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22217 in the following applications:

Publications
Read Publications using
NBP2-22217 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • WB
    3 publications

Reactivity Notes

Does not react with rat. Mouse reactivity reported in scientific literature (PMID: 27863209)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LAMP-2/CD107b Antibody (H4B4)

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMPB
  • LGP110
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

LAMP2 (Lysosome-associate protein 2) is one of a family of membrane glycoproteins whose function is to provide selectins with carbohydrate ligands. LAMP2 has been implicated in tumor metastasis, as well as overall protection, maintenance and adhesion of the lysosome. Specifically, LAMP2 may protect the lysosomal membrane from auto digestion, and also maintain the acidic environment necessary for the lysosome to function correctly. On a subcellular level, LAMP2 is expressed as a single-pass type I membrane protein, shuttling between endosomes, lysosomes, and the plasma membrane. Mutations in LAMP2 lead to a lysosomal glycogen storage disease known as "Danon Disease". Recently, caspase activation has been suggested in the extracellular export of autophagic vesicles, such as LAMP2 (PMID: 22692030).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-2/CD107b Antibody (NBP2-22217)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
WB
(3)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP2-22217 Applications Species
Lee WH, Higuchi H, Ikeda S et al. Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. Elife. Nov 15 2016 [PMID: 27863209] (WB, Mouse) WB Mouse
Nighot PK, Hu CA, Ma TY. Autophagy enhances intestinal epithelial tight junction barrier function by targeting claudin-2 protein degradation. J Biol Chem 2015 Mar 13 [PMID: 25616664] (Human) Human
Choi Yp, Head Mw, Ironside Jw, Priola Sa. Uptake and Degradation of Protease-Sensitive and -Resistant Forms of Abnormal Human Prion Protein Aggregates by Human Astrocytes. Am J Pathol 2014 Sep 29 [PMID: 25280631] (ICC/IF, Human)

Details:
Using the Alexa Fluor (R) 488 conjugated version of NBP2-22217, catalog number NBP2-22217AF488.
ICC/IF Human
Bhandari D, Trejo J, Benovic JL, Marchese A. Arrestin-2 interacts with the ubiquitin-protein isopeptide ligase atrophin-interacting protein 4 and mediates endosomal sorting of the chemokine receptor CXCR4. J Biol Chem. 2007 Dec 21 [PMID: 17947233] (ICC/IF, Human) ICC/IF Human
Iwata A, Riley BE, Johnston JA, Kopito RR. HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem. 2005 Dec 2 [PMID: 16192271] (ICC/IF, Human) ICC/IF Human
Zhang Y, Higashide W, Dai S et al. Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1. J Biol Chem. 2005 Nov 18 [PMID: 16176924] (WB, Human) WB Human
Kunzli BM, Berberat PO, Zhu ZW et al. Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma. Cancer. 2002 Jan 1 [PMID: 11815981] (IHC, Human) IHC Human
Nishino I, Fu J, Tanji et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000 Aug 24 [PMID: 10972294] (WB, IHC, Human) WB, IHC Human
Warren RA, Green FA, Stenberg PE, Enns CA. Distinct saturable pathways for the endocytosis of different tyrosine motifs. J Biol Chem. 1998 Jul 3 [PMID: 9642270]
Garcia-del Portillo F, Stein MA, Finlay BB. Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell. Infect Immun. 1997 Jan [PMID: 8975888] (ICC/IF, Human) ICC/IF Human

Review for LAMP-2/CD107b Antibody (NBP2-22217) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22217:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 06/03/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage whole cell lysate
SpeciesHuman
LotA-3

Blocking

Blocking Details5% Skimmed milk in 0.1% Tween20 1XPBS, 1h, RT

Primary Anitbody

Dilution Ratio1:5000 in 5% Skimmed milk in 0.1% Tween20 1XPBS, overnight at 4 degree celcius

Secondary Antibody

Secondary Manufacturer Cat#WB7104
Secondary ConcentrationReady to use solution for secondary antibody

Details

Detection NotesWesternBreeze Chemiluminiscent Detection kit using automated X Ray developing system exposure time is 30 seconds to 1 min

Comments

CommentsThis antibody is very sensitive so final dilution can be higher than 5000 in cell type dependent manner and exposure time during detection has to be very low.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-22217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-22217AF405
Alexa Fluor 488 Labeled NBP2-22217AF488
Alexa Fluor 647 Labeled NBP2-22217AF647
Alexa Fluor 700 Labeled NBP2-22217AF700
Biotin Labeled NBP2-22217B
DyLight 350 Labeled NBP2-22217UV
DyLight 405 Labeled NBP2-22217V
DyLight 488 Labeled NBP2-22217G
DyLight 550 Labeled NBP2-22217R
DyLight 650 Labeled NBP2-22217C
DyLight 680 Labeled NBP2-22217FR
DyLight 755 Labeled NBP2-22217IR
HRP Labeled NBP2-22217H
PE Labeled NBP2-22217PE
PerCP Labeled NBP2-22217PCP

Additional LAMP-2/CD107b Products

Bioinformatics Tool for LAMP-2/CD107b Antibody (NBP2-22217)

Discover related pathways, diseases and genes to LAMP-2/CD107b Antibody (NBP2-22217). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for LAMP-2/CD107b Antibody (NBP2-22217)

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Pathways for LAMP-2/CD107b Antibody (NBP2-22217)

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PTMs for LAMP-2/CD107b Antibody (NBP2-22217)

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Research Areas for LAMP-2/CD107b Antibody (NBP2-22217)

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Blogs on LAMP-2/CD107b.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

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Recent Reviews

5
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1
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2
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1
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Anonymous
06/03/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol LAMP2
Entrez