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LAMP-2/CD107b Antibody (H4B4) - BSA Free


Genetic Strategies: Knockout Validated: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP2 knockout (KO) HeLa cell line. PVDF membrane more
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Immunofluorescence: [Alexa Fluor® 647] [NBP2-22217AF647] - T98G glioblastoma cells grown on #1.5 coverglass stained with a more
Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells more
Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more
Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following more
Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in more
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The more
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody more
ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted more
Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. (E) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following more

Product Details

Reactivity Hu, Mu, Ch, Rt(-)Species Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, WB, CyTOF-ready, KO
BSA Free
1.0 mg/ml
Validated by:

Genetic Strategies


Order Details

LAMP-2/CD107b Antibody (H4B4) - BSA Free Summary

Additional Information
Clone H4B4 was used by HLDA to establish CD designation.
LAMP-2/CD107b Antibody (H4B4) was made using a human adherent spleen cells.
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Late Endosome / Lysosome marker
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunoblotting reported in scientific literature (PMID 29643474)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Knockout Validated
  • Simple Western 1:25
  • Western Blot 1:1000
Application Notes
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human Bladder Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 4.5
NBP2-22217 in the following applications:

Read Publications using
NBP2-22217 in the following applications:

  • EM
    2 publications
  • IB
    1 publication
  • 10 publications
  • IF
    2 publications
  • 2 publications
  • 1 publication
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27863209). Chicken reactivity reported in scientific literature (PMID: 30298003).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for LAMP-2/CD107b Antibody (H4B4) - BSA Free

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMP2A
  • LAMP-2A
  • LGP110
  • LGP-96
  • Lysosomal Associated Membrane Protein 2
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2


LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). LAMP-2 protein is encoded by nine exons, with the first 8 exons and a portion of exon 9 encoding the highly glycosylated protein domains within the lysosomal lumen. The transmembrane and cytosolic carboxy-terminal domains of LAMP-2 are encoded by the remaining sequence of exon 9 and conform the receptor for targeting proteins to the lysosome. Splicing of exon 9 in the LAMP-2 pre mRNA leads to various splice forms with distinct cytosolic domains. Three splice variants, LAMP-2A, -2B and -2C, have been identified which shuttle between the plasma membrane, endosomal compartment and lysosomes (1). Tissue specific expression has been described for each LAMP-2 splice variant, with LAMP-2A being more ubiquitously expressed (e.g., placenta, lung, liver, pancreas and prostate), LAMP-2B predominantly expressed in skeletal muscle and LAMP-2C in brain tissue (1). All LAMP-2 splice variants participate in lysosomal degradation processes. LAMP-2A is the only variant that serves as a receptor targeting proteins for lysosomal degradation in chaperone-mediated autophagy (2,3). LAMP-2B is essential for macroautophagy in cardiomyocytes, where it facilitates autophagosome-lysosome fusion. LAMP-2B mutations underscore the myopathy and severe hypertrophic cardiomyopathy in Danon disease which results from deficits in autophagy (1, 4). Vasculopathy of coronary and cerebral arteries is a rare phenotype in Danon patients that is also associated with deficient autophagy processing of proteins and cellular organelles (5). LAMP2C serves as a receptor for DNA and RNA, facilitating their lysosomal degradation through DNA-autophagy and RNA-autophagy, respectively (1).


1. Rowland, T. J., Sweet, M. E., Mestroni, L., & Taylor, M. R. G. (2016). Danon disease - dysregulation of autophagy in a multisystem disorder with cardiomyopathy. Journal of Cell Science.

2. Alfaro, I. E., Albornoz, A., Molina, A., Moreno, J., Cordero, K., Criollo, A., & Budini, M. (2019). Chaperone mediated autophagy in the crosstalk of neurodegenerative diseases and metabolic disorders. Frontiers in Endocrinology.

3. Schneider, J. L., & Cuervo, A. M. (2014). Autophagy and human disease: Emerging themes. Current Opinion in Genetics and Development.

4. Chi, C., Leonard, A., Knight, W. E., Beussman, K. M., Zhao, Y., Cao, Y., Song, K. (2019). LAMP-2B regulates human cardiomyocyte function by mediating autophagosome lysosome fusion. Proceedings of the National Academy of Sciences of the United States of America.

5. Nguyen, H. T., Noguchi, S., Sugie, K., Matsuo, Y., Nguyen, C. T. H., Koito, H., Tsukaguchi, H. (2018). Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Scientific Reports.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB
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Applications: BA
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Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
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Publications for LAMP-2/CD107b Antibody (NBP2-22217)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 8 applications: EM, IB, ICC/IF, IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NBP2-22217 Applications Species
Fang R, Ming T, Ng JPL et al. Ciliatoside A, isolated from Peristrophe japonica, inhibits HBsAg expression and cccDNA transcription by inducing autophagy Antiviral research 2022-12-07 [PMID: 36496141]
Lee H, Nguyen Hoang AT, Lee SJ. Ginsenoside protopanaxadiol protects adult retinal pigment epithelial-19 cells from chloroquine by modulating autophagy and apoptosis PLOS ONE 2022-12-01 [PMID: 36454967]
Pillai S, Mahmud I, Mahar R et al. Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy Cell reports 2022-05-10 [PMID: 35545051] (ICC/IF, Human) ICC/IF Human
Fathi A, Mathivanan S, Kong L et al. Chemically induced senescence in human stem cell-derived neurons promotes phenotypic presentation of neurodegeneration Aging Cell 2022-01-01 [PMID: 34953016]
Nanou A, Bourbouli M, Vetrano S et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis Cell reports 2021-05-25 [PMID: 34038728] (IHC-P, Mouse) IHC-P Mouse
Hirata H, Xu X, Nishioka K, et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-02-01 [PMID: 33484199]
Yuniati L, Lauriola A, Gerritsen M et al. Ubiquitylation of the ER-Shaping Protein Lunapark via the CRL3KLHL12 Ubiquitin Ligase Complex Cell Rep 2020-05-19 [PMID: 32433973]
Price C, Jones S, Mihelcic M et al. Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector Cell Host Microbe 2020-03-30 [PMID: 32220647]
Ahmed M, Baumgartner R, Aldi S et al. Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors Int J Nanomedicine 2019-05-21 [PMID: 31190821] (IF, Human) IF Human
Chang-Graham AL, Danhof HA, Engevik MA et al. Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions Mol. Cell. Biol. 2019-05-06 [PMID: 31061094]
Show All 31 Publications.

Reviews for LAMP-2/CD107b Antibody (NBP2-22217) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-22217:
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Images Ratings Applications Species Date Details
ELISA LAMP-2/CD107b NBP2-22217
reviewed by:
Verified Customer
ELISA Human 06/05/2018


Sample TestedSVG-A cells
reviewed by:
Verified Customer
WB Human 06/03/2016


ApplicationWestern Blot
Sample Testedhuman macrophage whole cell lysate

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-22217) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Verified Customer
Application: ELISA
Species: Human

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Application: WB
Species: Human


Gene Symbol LAMP2