LAMP-2/CD107b Antibody (H4B4)


Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP2 expression in HeLa, Jurkat, U937, HepG2 whole cell lysates, human kidney, lung, liver and placenta tissue extracts.
Immunocytochemistry/ Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - IHC staining of LAMP2 in human liver using DAB with hematoxylin counterstain.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis using the FITC conjugate of NBP2-22217. Staining of 5ul overlay with isotype on U937 Cells.
Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using LAMP2 antibody (dark blue). Isotype control shown in orange. An antibody concentration more
Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Simple Western lane view shows a specific band for LAMP2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the more

Product Details

Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

LAMP-2/CD107b Antibody (H4B4) Summary

Human adherent spleen cells.
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Late Endosome / Lysosome marker
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1:1000
  • Simple Western 1:25
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
In Western blot, bands may be seen at ~40 kDa and 45 kDa representing the unglycosylated isoforms of LAMP2 and ~110 kDa representing the glycosylated form.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
NBP2-22217 in the following applications:

Read Publications using
NBP2-22217 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • WB
    2 publications

Reactivity Notes

Does not react with mouse or rat.

Alternate Names for LAMP-2/CD107b Antibody (H4B4)

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LGP110
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2


LAMP2 (Lysosome-associate protein 2) is one of a family of membrane glycoproteins whose function is to provide selectins with carbohydrate ligands. LAMP2 has been implicated in tumor metastasis, as well as overall protection, maintenance and adhesion of the lysosome. Specifically, LAMP2 may protect the lysosomal membrane from auto digestion, and also maintain the acidic environment necessary for the lysosome to function correctly. On a subcellular level, LAMP2 is expressed as a single-pass type I membrane protein, shuttling between endosomes, lysosomes, and the plasma membrane. Mutations in LAMP2 lead to a lysosomal glycogen storage disease known as "Danon Disease". Recently, caspase activation has been suggested in the extracellular export of autophagic vesicles, such as LAMP2 (PMID: 22692030).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-2/CD107b Antibody (NBP2-22217)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Showing Publications 1 - 9 of 9.
Publications using NBP2-22217 Applications Species
Nighot PK, Hu CA, Ma TY. Autophagy enhances intestinal epithelial tight junction barrier function by targeting claudin-2 protein degradation. J Biol Chem 2015 Mar 13 [PMID:25616664] (Human) Human
Choi Yp, Head Mw, Ironside Jw, Priola Sa. Uptake and Degradation of Protease-Sensitive and -Resistant Forms of Abnormal Human Prion Protein Aggregates by Human Astrocytes. Am J Pathol 2014 Sep 29 [PMID:25280631] (ICC/IF, Human) ICC/IF Human
Bhandari D, Trejo J, Benovic JL, Marchese A. Arrestin-2 interacts with the ubiquitin-protein isopeptide ligase atrophin-interacting protein 4 and mediates endosomal sorting of the chemokine receptor CXCR4. J Biol Chem. 2007 Dec 21 [PMID:17947233] (ICC/IF, Human) ICC/IF Human
Iwata A, Riley BE, Johnston JA, Kopito RR. HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. J Biol Chem. 2005 Dec 2 [PMID:16192271] (ICC/IF, Human) ICC/IF Human
Zhang Y, Higashide W, Dai S et al. Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1. J Biol Chem. 2005 Nov 18 [PMID:16176924] (WB, Human) WB Human
Kunzli BM, Berberat PO, Zhu ZW et al. Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma. Cancer. 2002 Jan 1 [PMID:11815981] (IHC, Human) IHC Human
Nishino I, Fu J, Tanji et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000 Aug 24 [PMID:10972294] (WB, IHC, Human) WB, IHC Human
Warren RA, Green FA, Stenberg PE, Enns CA. Distinct saturable pathways for the endocytosis of different tyrosine motifs. J Biol Chem. 1998 Jul 3 [PMID:9642270]
Garcia-del Portillo F, Stein MA, Finlay BB. Release of lipopolysaccharide from intracellular compartments containing Salmonella typhimurium to vesicles of the host epithelial cell. Infect Immun. 1997 Jan [PMID:8975888] (ICC/IF, Human) ICC/IF Human

Reviews for LAMP-2/CD107b Antibody (NBP2-22217) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22217:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 06/03/2016


ApplicationWestern Blot
Sample Testedhuman macrophage whole cell lysate


Blocking Details5% Skimmed milk in 0.1% Tween20 1XPBS, 1h, RT

Primary Anitbody

Dilution Ratio1:5000 in 5% Skimmed milk in 0.1% Tween20 1XPBS, overnight at 4 degree celcius

Secondary Antibody

Secondary Manufacturer Cat#WB7104
Secondary ConcentrationReady to use solution for secondary antibody


Detection NotesWesternBreeze Chemiluminiscent Detection kit using automated X Ray developing system exposure time is 30 seconds to 1 min


CommentsThis antibody is very sensitive so final dilution can be higher than 5000 in cell type dependent manner and exposure time during detection has to be very low.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-22217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-22217AF405
Alexa Fluor (R) 488 Labeled NBP2-22217AF488
Alexa Fluor (R) 647 Labeled NBP2-22217AF647
Alexa Fluor (R) 700 Labeled NBP2-22217AF700
Biotin Labeled NBP2-22217B
DyLight 350 Labeled NBP2-22217UV
DyLight 405 Labeled NBP2-22217V
DyLight 405LS Labeled NBP2-22217V3
DyLight 488 Labeled NBP2-22217G
DyLight 550 Labeled NBP2-22217R
DyLight 650 Labeled NBP2-22217C
DyLight 680 Labeled NBP2-22217FR
DyLight 755 Labeled NBP2-22217IR
HRP Labeled NBP2-22217H
PE Labeled NBP2-22217PE
PerCP Labeled NBP2-22217PCP

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Blogs on LAMP-2/CD107b.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

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Product PDFs


Gene Symbol LAMP2

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